Double‐stranded RNAs targeting HvRPS18 and HvRPL13 reveal potential targets for pest management of the 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata

Jing Lu; Wei Guo; Shimin Chen; Mujuan Guo; Baoli Qiu; Chunxiao Yang; Youjun Zhang; Huipeng Pan

doi:10.1002/ps.5809

journal article Mar 12, 2020

Double‐stranded RNAs targeting HvRPS18 and HvRPL13 reveal potential targets for pest management of the 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata

Jing Lu

Wei Guo

Shimin Chen Mujuan Guo Baoli Qiu

Chunxiao Yang

Youjun Zhang

Huipeng Pan

Pest Management Science Vol. 76 No. 8 pp. 2663-2673 · Wiley

View at Publisher Save 10.1002/ps.5809

Abstract

AbstractBACKGROUNDRNA interference (RNAi) is a potential tool for plant protection against insect pests. The great challenge for effective pest control using RNAi in the field is the development of efficient and reliable methods for the production and delivery of double‐stranded RNA (dsRNA).RESULTSIn the present study, we investigated the potential of feeding in vitro synthesized or bacterially expressed dsRNA to populations of the 28‐spotted ladybeetle Henosepilachna vigintioctopunctata as a method of biological pest control. Ingestion of in vitro synthesized dsHvRPS18 or dsHvRPL13 led to significant down‐regulation of the ribosomal protein‐encoding genes HvRPS18 and HvRPL13, respectively, and significantly decreased the survival of H. vigintioctopunctata. Such silencing of HvRPS18 or HvRPL13 expression appeared to be partially dose‐dependent and also inhibited the growth of H. vigintioctopunctata and significantly suppressed the expression of digestive enzyme‐related genes. Finally, ingestion of bacterially expressed dsHvRPS18 or dsHvRPL13 induced significant mortality in the first and third instars, and in adults.CONCLUSIONThe effectiveness of RNAi‐based gene silencing in H. vigintioctopunctata provides a powerful reverse genetic tool for the functional annotation of its genes. This study demonstrates that HvRPS18 and HvRPL13 represent candidate genes for RNAi‐based biological control of H. vigintioctopunctata. © 2020 Society of Chemical Industry

Topics

No keywords indexed for this article. Browse by subject →

References

48

[1]

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Andrew Fire, SiQun Xu, Mary K. Montgomery et al.

Nature 10.1038/35888

[2]

RNAi: Future in insect management

John P. Burand, Wayne B. Hunter

Journal of Invertebrate Pathology 10.1016/j.jip.2012.07.012

[3]

10.1007/s12010-013-0399-4

[4]

10.1016/j.tibtech.2017.04.009

[5]

Control of coleopteran insect pests through RNA interference

James A Baum, Thierry Bogaert, William Clinton et al.

Nature Biotechnology 10.1038/nbt1359

[6]

Ingested double-stranded RNAs can act as species-specific insecticides

Steven Whyard, Aditi D. Singh, Sylvia Wong

Insect Biochemistry and Molecular Biology 10.1016/j.ibmb.2009.09.007

[7]

10.1126/science.1261680

[8]

10.1002/ps.4813

[9]

10.3389/fpls.2019.00064

[10]

10.1002/ps.2048

[11]

10.1016/j.chemosphere.2016.07.014

[12]

10.1146/annurev-ento-011019-025224

[13]

Zhou L "Population dynamic of Henosepilachna vigintioctopunctata in different host plants in Jianghan plain" China J Northern Hort (2015)

[14]

PangXFandMaoJL Economic Insects of China 14 Coleoptera‐Coccinellidae II Science Press Beijing pp. 108–112 (1979).

[15]

10.1093/jisesa/iey067

[16]

Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target

Julia Ulrich, Van Anh Dao, Upalparna Majumdar et al.

BMC Genomics 10.1186/s12864-015-1880-y

[17]

10.1074/jbc.ra117.001553

[18]

10.1016/j.ibmb.2017.05.008

[19]

10.3390/ijms19041079

[20]

10.1007/s10340-019-01097-6

[21]

10.1007/s10340-013-0541-7

[22]

10.1073/pnas.1809381115

[23]

10.1002/ps.5370

[24]

10.3389/fphys.2019.01371

[25]

10.1152/physiolgenomics.00233.2004

[26]

10.3389/fpls.2016.01028

[27]

10.3389/fpls.2017.00242

[28]

10.1016/j.molcel.2008.12.010

[29]

10.1007/s10493-014-9772-4

[30]

10.1016/j.ttbdis.2018.01.015

[31]

10.1007/s004380050590

[32]

10.1111/j.1365-2583.2009.00926.x

[33]

10.1016/j.jinsphys.2016.05.001

[34]

10.3389/fphys.2019.00981

[35]

10.1371/journal.pone.0125868

[36]

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen

Methods 10.1006/meth.2001.1262

[37]

10.1016/0168-9525(87)90295-2

[38]

10.3389/fpls.2015.00958

[39]

10.1016/j.envint.2013.02.010

[40]

10.1038/nbt1180

[41]

10.4161/gmcr.19762

[42]

US EPA (2001)

[43]

10.1016/j.biocontrol.2018.10.001

[44]

10.1073/pnas.1232238100

[45]

10.1016/j.chemosphere.2015.09.068

[46]

Yu JY "Functional response of Campylomma chinensis Schuh preying on eggs of Henosepilachna vigintioctopunctata" China Plant Prot (2012)

[47]

Wang GH "Functional responses of Pediobius foveolatus to Henosepilachna vigintioctopunctata" Chin J Ecol (2005)

[48]

10.1016/j.peptides.2013.12.014

Metrics

27

Citations

48

References

Details

Published: Mar 12, 2020
Vol/Issue: 76(8)
Pages: 2663-2673
License: View

Authors

J

Jing Lu

Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province South China Agricultural University Guangzhou China

W

Wei Guo

Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province South China Agricultural University Guangzhou China

S

Shimin Chen

Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province South China Agricultural University Guangzhou China

M

Mujuan Guo

Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province South China Agricultural University Guangzhou China

B

Baoli Qiu

Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province South China Agricultural University Guangzhou China; Lingnan Guangdong Laboratory of Modern Agriculture Guangzhou China

C

Chunxiao Yang

Lingnan Guangdong Laboratory of Modern Agriculture Guangzhou China

Y

Youjun Zhang

Department of Plant Protection, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing China

H

Huipeng Pan

Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province South China Agricultural University Guangzhou China; Lingnan Guangdong Laboratory of Modern Agriculture Guangzhou China

Funding

National Natural Science Foundation of China Award: 31972269

South China Agricultural University

Cite This Article

Jing Lu, Wei Guo, Shimin Chen, et al. (2020). Double‐stranded RNAs targeting HvRPS18 and HvRPL13 reveal potential targets for pest management of the 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata. Pest Management Science, 76(8), 2663-2673. https://doi.org/10.1002/ps.5809

Double‐stranded RNAs targeting HvRPS18 and HvRPL13 reveal potential targets for pest management of the 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata

You May Also Like