Gastrodin Ameliorates Oxidative Stress and Proinflammatory Response in Nonalcoholic Fatty Liver Disease through the AMPK/Nrf2 Pathway

Li‐Li Qu; Bin Yu; Zheng Li; Wen‐Xiao Jiang; Jian‐Dong Jiang; Wei‐Jia Kong

doi:10.1002/ptr.5541

journal article Dec 04, 2015

Gastrodin Ameliorates Oxidative Stress and Proinflammatory Response in Nonalcoholic Fatty Liver Disease through the AMPK/Nrf2 Pathway

Li‐Li Qu Bin Yu

Zheng Li

Wen‐Xiao Jiang Jian‐Dong Jiang Wei‐Jia Kong

Phytotherapy Research Vol. 30 No. 3 pp. 402-411 · Wiley

View at Publisher Save 10.1002/ptr.5541

Abstract

This study was designed to investigate the antioxidative, antiinflammatory and metabolism‐regulating effects of gastrodin (GSTD) in the treatment of nonalcoholic fatty liver disease (NAFLD). Oleic acid (OA) was used to induce steatosis in HL‐7702 cells; a high‐fat or high‐fat and high‐cholesterol diet was used to induce NAFLD in mice and rats. Our results showed that GSTD significantly increased hepatic superoxide dismutase (SOD) but decreased reactive oxygen species (ROS)/malondialdehyde (MDA) and reduced the mRNA levels of proinflammatory cytokines both in vitro and in vivo. GSTD promoted the phosphorylation of nuclear factor erythroid‐2‐related factor‐2 (Nrf2) at serine (Ser) 40, stimulated its nuclear translocation and increased hepatic expression of heme oxygenase‐1 (HO‐1). GSTD activated AMP‐activated protein kinase (AMPK), suppressed hepatic steatosis, lowered serum triglyceride (TG)/glucose and decreased body weight gain in animals with NAFLD. The stimulating effects of GSTD on the Nrf2 pathway as well as its antioxidative/antiinflammatory activities were abolished by compound C in OA‐treated HL‐7702 cells. In summary, our results demonstrate that GSTD activates the AMPK/Nrf2 pathway, ameliorates oxidative stress/proinflammatory response and improves lipid metabolism in NAFLD. Our findings may support the future clinical application of GSTD for the treatment of NAFLD to reduce hepatic steatosis, oxidative stress and proinflammatory response. Copyright © 2015 John Wiley & Sons, Ltd.

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Details

Published: Dec 04, 2015
Vol/Issue: 30(3)
Pages: 402-411
License: View

Authors

L

Li‐Li Qu

State Key Laboratory of Bioactive Natural Products and Function, Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

B

Bin Yu

Department of Pharmacology, Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

Z

Zheng Li

Department of Pharmacology, Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

W

Wen‐Xiao Jiang

Department of Microbiology and Molecular Genetics Emory University Atlanta GA USA

J

Jian‐Dong Jiang

State Key Laboratory of Bioactive Natural Products and Function, Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

W

Wei‐Jia Kong

Department of Pharmacology, Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

Funding

National Natural Science Foundation of China Award: 81321004

Cite This Article

Li‐Li Qu, Bin Yu, Zheng Li, et al. (2015). Gastrodin Ameliorates Oxidative Stress and Proinflammatory Response in Nonalcoholic Fatty Liver Disease through the AMPK/Nrf2 Pathway. Phytotherapy Research, 30(3), 402-411. https://doi.org/10.1002/ptr.5541

Gastrodin Ameliorates Oxidative Stress and Proinflammatory Response in Nonalcoholic Fatty Liver Disease through the AMPK/Nrf2 Pathway

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