Mesenchymal TGF-βSignaling Orchestrates Dental Epithelial Stem Cell Homeostasis Through Wnt Signaling

Guan Yang; Jian Zhou; Yan Teng; Jing Xie; Jun Lin; Xizhi Guo; Yuanrong Gao; Miao He; Xiao Yang; Songlin Wang

doi:10.1002/stem.1772

journal article Oct 14, 2014

Mesenchymal TGF-βSignaling Orchestrates Dental Epithelial Stem Cell Homeostasis Through Wnt Signaling

Guan Yang Jian Zhou

Yan Teng

Jing Xie

Jun Lin

Xizhi Guo Yuanrong Gao Miao He

Xiao Yang

Songlin Wang

Stem Cells Vol. 32 No. 11 pp. 2939-2948 · Oxford University Press (OUP)

View at Publisher Save 10.1002/stem.1772

Abstract

AbstractIn mouse, continuous growth of the postnatal incisor is coordinated by two populations of multipotent progenitor cells, the dental papilla mesenchymal cells and dental epithelial stem cells, residing at the proximal end of the incisor, yet the molecular mechanism underlying the cooperation between mesenchymal and epithelial cells is largely unknown. Here, transforming growth factor-β (TGF-β) type II receptor (Tgfbr2) was specifically deleted within the postnatal dental papilla mesenchyme. The Tgfbr2-deficient mice displayed malformed incisors with wavy mineralized structures at the labial side as a result of increased differentiation of dental epithelial stem cells. We found that mesenchymal Tgfbr2 disruption led to upregulated expression of Wnt5a and downregulated expression of Fgf3/10 in the mesenchyme, both of which synergistically enhanced Lrp5/6-β-catenin signaling in the cervical loop epithelium. In accord with these findings, mesenchyme-specific depletion of the Wnt transporter gene Wls abolished the aberrant mineralized structures caused by Tgfbr2 deletion. Thus, mesenchymal TGF-β signaling provides a unifying mechanism for the homeostasis of dental epithelial stem cells via a Wnt signaling-mediated mesenchymal-epithelial cell interaction. Stem Cells 2014;32:2939–2948

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References

Details

Published: Oct 14, 2014
Vol/Issue: 32(11)
Pages: 2939-2948
License: View

Authors

G

Guan Yang

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology, Beijing, People’s Republic of China; State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases Institute of Biotechnology, Beijing, People’s Republic of China

J

Jian Zhou

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology, Beijing, People’s Republic of China

Y

Yan Teng

State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases Institute of Biotechnology, Beijing, People’s Republic of China

J

Jing Xie

State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases Institute of Biotechnology, Beijing, People’s Republic of China

J

Jun Lin

State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases Institute of Biotechnology, Beijing, People’s Republic of China

X

Xizhi Guo

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders Shanghai Jiao Tong University, Shanghai, People’s Republic of China

Y

Yuanrong Gao

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology, Beijing, People’s Republic of China

M

Miao He

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology, Beijing, People’s Republic of China

X

Xiao Yang

State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases Institute of Biotechnology, Beijing, People’s Republic of China

S

Songlin Wang

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction Capital Medical University School of Stomatology, Beijing, People’s Republic of China

Cite This Article

Guan Yang, Jian Zhou, Yan Teng, et al. (2014). Mesenchymal TGF-βSignaling Orchestrates Dental Epithelial Stem Cell Homeostasis Through Wnt Signaling. Stem Cells, 32(11), 2939-2948. https://doi.org/10.1002/stem.1772

Mesenchymal TGF-βSignaling Orchestrates Dental Epithelial Stem Cell Homeostasis Through Wnt Signaling

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