An expanded tool kit for the auxin‐inducible degron system in budding yeast

Magdalena Morawska; Helle D. Ulrich

doi:10.1002/yea.2967

journal article Open Access Jul 23, 2013

An expanded tool kit for the auxin‐inducible degron system in budding yeast

Magdalena Morawska Helle D. Ulrich

Yeast Vol. 30 No. 9 pp. 341-351 · Wiley

View at Publisher Save 10.1002/yea.2967

Abstract

AbstractFusion of inducible degradation signals, so‐called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin‐inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR‐based genomic tagging strategies, allow for C‐ or N‐terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. © 2013 The Authors. Yeast published by John Wiley & Sons, Ltd.

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Details

Published: Jul 23, 2013
Vol/Issue: 30(9)
Pages: 341-351
License: View

Authors

M

Magdalena Morawska

Cancer Research UK London Research Institute Clare Hall Laboratories South Mimms UK

H

Helle D. Ulrich

Cancer Research UK London Research Institute Clare Hall Laboratories South Mimms UK; Institute of Molecular Biology Ackermannweg 4 55128 Mainz Germany

Cite This Article

Magdalena Morawska, Helle D. Ulrich (2013). An expanded tool kit for the auxin‐inducible degron system in budding yeast. Yeast, 30(9), 341-351. https://doi.org/10.1002/yea.2967

An expanded tool kit for the auxin‐inducible degron system in budding yeast

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