Investigating the growth and yield responses of Abelmoschus esculentus L. cv. Kashi Kranti (VRO-22) grown under the co-application of sewage-sludge and fly ash in soil

Rajeev Pratap Singh; Meenu Gautam; Bhavisha Sharma; Richa Kothari; Anita Singh

doi:10.1007/s42398-025-00391-7

journal article Nov 17, 2025

Investigating the growth and yield responses of Abelmoschus esculentus L. cv. Kashi Kranti (VRO-22) grown under the co-application of sewage-sludge and fly ash in soil

Rajeev Pratap Singh

Meenu Gautam Bhavisha Sharma Richa Kothari

Anita Singh

Environmental Sustainability Vol. 9 No. 1 pp. 183-201 · Springer Science and Business Media LLC

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References

91

[1]

Aboudrar W, Schwartz C, Benizri E, Morel JL, Boularbah A (2007) Soil microbial diversity as affected by the rhizosphere of the hyperaccumulator Thlaspi caerulescens under natural conditions. Int J Phytoremediation 9(1):41–52 10.1080/15226510601139417

[2]

Ahirwar CS, Singh AP, Nath R, Verty P (2021) Assessments effect of nitrogen and phosphorus on the phenological and fruit characters of Okra (Abelmoschus esculentus L). International J Curr Microbiol Appl Science 10(02):1918–1925 10.20546/ijcmas.2021.1002.229

[3]

Allen SE, Grimshaw HM, Rowland AP (1986) Chemical analysis. In: Moore PD, Chapman SB (eds) Methods in plant ecology. Blackwill Scientific Publication, Oxford, pp 285–344

[4]

Allison SD, Jastrow JD (2006) Activities of extracellular enzymes in physically isolated fractions of restored grassland soils. Soil Biol Biochem 38(11):3245–3256 10.1016/j.soilbio.2006.04.011

[5]

Alloway B, Ayres DC (1997) Chemical principles of environmental pollution. CRC

[6]

Antonkiewicz J, Popławska A, Kołodziej B, Ciarkowska K, Gambuś F, Bryk M, Babula J (2020) Application of Ash and municipal sewage sludge as macronutrient sources in sustainable plant biomass production. J Environ Manage 264:110450 10.1016/j.jenvman.2020.110450

[7]

Ashraf MA, Rasheed R, Zafar S, Iqbal M, Saqib ZA (2021) Menadione sodium bisulfite neutralizes chromium phytotoxic effects in Okra by regulating cytosolutes, lipid peroxidation, antioxidant system and metal uptake. Int J Phytoremediation 23(7):736–746

[8]

Awashthi SK (2000) Central and state rules as amended for 1999: prevention of food adulteration act no 37 of 1954. Ashoka Law House, New Delhi

[9]

Balkrishna A, Gautam AK, Sharma N, Arya V, Khelwade V (2024) Growth and yield potential of sludge-based organic fertilizers on bell pepper Capsicum annum. Asian J Agric, 8(1) 10.13057/asianjagric/g080103

[10]

Rapid determination of free proline for water-stress studies

L. S. Bates, R. P. Waldren, I. D. Teare

Plant and Soil 1973 10.1007/bf00018060

[11]

Bhattacharya T, Pandey SK, Pandey VC, Kumar A (2021) Potential and safe utilization of fly Ash as fertilizer for Pisum sativum L. Grown in phytoremediated and non-phytoremediated amendments. Environ Sci Pollut Res 28(36):50153–50166 10.1007/s11356-021-14179-9

[12]

Biswal T, Shadangi KP, Sarangi PK (2023) Fly ash derived catalyst for biodiesel production. Biorefinery Prod Fuels Platf Chemicals, 203–232 10.1002/9781119724872.ch9

[13]

Bray HG, Thorpe W (1954) Analysis of phenolic compounds of interest in metabolism. Methods Biochem Anal 1:27–52 10.1002/9780470110171.ch2

[14]

Britton C, Mehley AC (1955) Assay of catalase and peroxidase. Methods Enzymol 2:764–775 10.1016/s0076-6879(55)02300-8

[15]

Buchmann MF (2008) NOAA screening quick reference Tables, NOAA OR & R report 08–1. Office of Response and Restoration Division, National Oceanic and Atmospheric Administration, Seattle (WA), p 34

[16]

Celi GEA, Gratão PL, Lanza MGDB, Reis DAR (2023) Physiological and biochemical roles of ascorbic acid on mitigation of abiotic stresses in plants. Plant Physiol Biochem 107970 10.1016/j.plaphy.2023.107970

[17]

Central Pollution Control Board, Government of India (2024) https://cpcb.nic.in/uploads/flyash/MOM_6_Ash_Utilisation_Mission.pdf#:~:text=iv.%20Out%20of%20121.1%20LMT%20ash%20generation,10.2%20LMT%20supplied%20to%20brick%20manufacturers.%20v. Accessed on 14.07.2025

[18]

Chowdhury NS, Jamaly S, Farjana F, Begum N, Zenat EA (2019) A review on ethnomedicinal, pharmacological, phytochemical and pharmaceutical profile of lady’s finger (Abelmoschus esculentus L.) plant. Pharmacol Pharm 10(02):94–108 10.4236/pp.2019.102008

[19]

Cox MS, Gerard PD, Wardlaw MC, Abshire MJ (2003) Variability of selected soil properties and their relationships with soybean yield. Soil Sci Soc Am J 67(4):1296–1302 10.2136/sssaj2003.1296

[20]

Criado M, Vicent M, García-Ten FJ (2022) Reactivation of alkali-activated materials made up of fly ashes from a coal power plant. Clean Mater 3:100043 10.1016/j.clema.2022.100043

[21]

Dhanker R, Chaudhary S, Goyal S, Garg VK (2021) Influence of urban sewage sludge amendment on agricultural soil parameters. Environ Technol Innov 23:101642 10.1016/j.eti.2021.101642

[22]

Duxbury AC, Yentsch CS (1956) Plankton pigment nomographs. Canadian Science Publishing, Ottawa, ON, Canada

[23]

Egea I, Estrada Y, Flores FB, Bolarín MC (2022) Improving production and fruit quality of tomato under abiotic stress: genes for the future of tomato breeding for a sustainable agriculture. Environ Exp Bot 204:105086 10.1016/j.envexpbot.2022.105086

[24]

Eid EM, Shaltout KH, Alamri SA, Alrumman SA, Taher MA, El-Bebany AF, Nessem AA (2022) Planned application of sewage sludge recirculates nutrients to agricultural soil and improves growth of Okra (Abelmoschus esculentus (L.) Moench) plants. Sustainability 14(2):740 10.3390/su14020740

[25]

Emamverdian A, Ding Y, Mokhberdoran F, Xie Y (2015) Heavy metal stress and some mechanisms of plant defense response. Sci World J 20151:756120 10.1155/2015/756120

[26]

Fahey RC, Brown WC, Adams WB, Worsham MB (1978) Occurrence of glutathione in bacteria. J Bacteriol 133:1126–1129 10.1128/jb.133.3.1126-1129.1978

[27]

FAO/WHO (Codex Alimentarius Commission) (2001) Food additives and contaminants. Joint FAO/WHO Food Stand Program ALINORM 01/12A:1–289

[28]

Fernández-Jiménez A, Palomo A (2003) Characterisation of fly ashes. Potential reactivity as alkaline cements. Fuel 82(18):2259–2265 10.1016/s0016-2361(03)00194-7

[29]

Ferrentino R, Langone M, Fiori L, Andreottola G (2023) Full-scale sewage sludge reduction technologies: a review with a focus on energy consumption. Water 15(4):615 10.3390/w15040615

[30]

Gautam M, Agrawal M (2019) Effects of red mud addition in soil fertilized with cowdung manure on growth performance and metal accumulations in Brassica juncea cultivars kranti and Pusa bold. Commun Soil Sci Plant Anal 50(10):1214–1231 10.1080/00103624.2019.1614599

[31]

Gautam M, Pandey D, Agrawal SB, Agrawal M (2016) Metals from mining and metallurgical industries and their toxicological impacts on plants. Plant Responses Xenobiotics 231–272 10.1007/978-981-10-2860-1_10

[32]

Gautam M, Pandey D, Agrawal M (2017) Phytoremediation of metals using Lemongrass (Cymbopogon citratus (DC) Stapf.) grown under different levels of red mud in soil amended with biowastes. Int J Phytoremediation 19(6):555–562 10.1080/15226514.2016.1267701

[33]

Gautam M, Pandey B, Agrawal SB, Agrawal M (2022) Stabilization of metals in sludge-amended soil using red mud and its effects on yield and oil quality of Brassica juncea cultivar kranti. Environ Geochem Health 44(5):1431–1450 10.1007/s10653-021-01150-3

[34]

Ghaffarzadeh Z, Iranbakhsh A, Ebadi M (2021) Evaluation of potential physiological and molecular responses of Basil (Ocimum basilicum) to the application of Fe2O3 nanoparticles. J Nanostructure Chem 1–9 10.1007/s40097-021-00438-1

[35]

Gupta AK, Mishra RK, Sinha S, Lee BK (2010) Growth, metal accumulation and yield performance of Brassica campestris L. (cv. Pusa Jaikisan) grown on soil amended with tannery sludge/fly Ash mixture. Ecol Eng 36(8):981–991 10.1016/j.ecoleng.2010.04.003

[36]

Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198 10.1016/0003-9861(68)90654-1

[37]

Hegedüs A, Erdei S, Horváth G (2001) Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sci 160(6):1085–1093 10.1016/s0168-9452(01)00330-2

[38]

Hosen L (2021) Studies on bioaccumulation and mobilization of heavy metals in crops and vegetables grown around industrial environments. http://lib.buet.ac.bd:8080/xmlui/handle/123456789/5975. Accessed on 10.08.2024

[39]

Hunt R (1982) Plant growth curves. The functional approach to plant growth analysis. Edward Arnold, London, pp 248–pp

[40]

Huseynova IM, Rustamova SM, Aliyeva DR, Babayev HG, Aliyev JA (2016) Photosynthesis, antioxidant protection, and drought tolerance in plants. Drought stress tolerance in plants, Cell Physiol Biochem 1:349–378 10.1007/978-3-319-28899-4_15

[41]

Hussein MM, Alva AK (2014) Effects of zinc and ascorbic acid application on the growth and photosynthetic pigments of millet plants grown under different salinity. Agricultural Sci 5(13):1253

[42]

Jia Y, Tang S, Wang R, Ju X, Ding Y, Tu S, Smith DL (2010) Effects of elevated CO2 on growth, photosynthesis, elemental composition, antioxidant level, and phytochelatin concentration in Lolium multiflorum and Lolium perenne under Cd stress. J Hazard Mater 180(1–3):384–394 10.1016/j.jhazmat.2010.04.043

[43]

Keller T, Schwager H (1977) Air pollution and ascorbic acid. Eur J Plant Pathol 7:338–350 10.1111/j.1439-0329.1977.tb00603.x

[44]

Kerven GL, Menzies NW, Geyer MD (2000) Soil carbon determination by high temperature combustion: A comparison with dichromate oxidation procedures and the influence of charcoal and carbonate on the measured value. Commun Soil Sci Plant Anal 31:1935–1939 10.1080/00103620009370551

[45]

Khatana RNS, Thomas T, Swaroop N, Barthwal A (2023) Effect of incorporation of sewage sludge and fly Ash on soil Physico-Chemical properties and Okra in inceptisols of Prayagraj UP. Int J Environ Clim Change 13(4):111–117 10.9734/ijecc/2023/v13i41717

[46]

Kim IS, Kang KH, Johnson-Green P, Lee EJ (2003) Investigation of heavy metal accumulation in Polygonum thunbergii for phytoextraction. Environ Pollut 126(2):235–243 10.1016/s0269-7491(03)00190-8

[47]

Kramer KK, Liu J, Choudhuri S, Klaassen CD (1996) Induction of Metallothionein mRNA and protein in murine astrocyte cultures. Toxicol Appl Pharmcol 136(1):94–100 10.1006/taap.1996.0011

[48]

Li J, Zheng Q, Liu J, Pei S, Yang Z, Chen R, Tian T (2024) Bacterial–fungal interactions and response to heavy metal contamination of soil in agricultural areas. Front Microbiol 15:1395154 10.3389/fmicb.2024.1395154

[49]

Lopareva-Pohu A, Verdin A, Garçon G, Sahraoui ALH, Pourrut B, Debiane D, Shirali P (2011) Influence of fly Ash aided phytostabilisation of Pb, cd and Zn highly contaminated soils on Lolium perenne and Trifolium repens metal transfer and physiological stress. Environ Pollut 159(6):1721–1729 10.1016/j.envpol.2011.02.030

[50]

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

OliverH. Lowry, NiraJ. Rosebrough, A. Lewis Farr et al.

Journal of Biological Chemistry 1951 10.1016/s0021-9258(19)52451-6

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Details

Published: Nov 17, 2025
Vol/Issue: 9(1)
Pages: 183-201
License: View

Authors

Department of Pharmacy Kumaon University Bhimtal Campus Bhimtal 263136 Uttarakhand India

Funding

Raja Jwala Prasad Fellowship, IoE, BHU Award: R/SCRICC/RJP-PDF/23-24/7387

Cite This Article

Rajeev Pratap Singh, Meenu Gautam, Bhavisha Sharma, et al. (2025). Investigating the growth and yield responses of Abelmoschus esculentus L. cv. Kashi Kranti (VRO-22) grown under the co-application of sewage-sludge and fly ash in soil. Environmental Sustainability, 9(1), 183-201. https://doi.org/10.1007/s42398-025-00391-7

Investigating the growth and yield responses of Abelmoschus esculentus L. cv. Kashi Kranti (VRO-22) grown under the co-application of sewage-sludge and fly ash in soil

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