Efficiency of chitosan to induce the immune defense system of soybean plant to drought stress

Mervat Shamoon Sadak; Mohamed El-sayed El-Awadi; Mona Gergis Dawood; Yasser Refaai Abdel-Baky

doi:10.1007/s42535-026-01643-5

journal article Apr 08, 2026

Efficiency of chitosan to induce the immune defense system of soybean plant to drought stress

Mervat Shamoon Sadak Mohamed El-sayed El-Awadi Mona Gergis Dawood Yasser Refaai Abdel-Baky

Vegetos · Springer Science and Business Media LLC

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References

130

[1]

Afzal S, Chaudhary N, Singh NK (2021) Role of soluble sugars in metabolism and sensing under abiotic stress. Plant growth regulators: signaling under stress conditions. Springer International Publishing, Switzerland, pp 305–334. https://doi.org/10.1007/978-3-030-61153-8_14 10.1007/978-3-030-61153-8_14

[2]

Ahluwalia O, Singh PC, Bhatia R (2021) A review on drought stress in plants: implications, mitigation and the role of plant growth promoting rhizobacteria. Resour Environ Sustain 5:100032. https://doi.org/10.1016/j.resenv.2021.100032 10.1016/j.resenv.2021.100032

[3]

Albalasmeh AA, Berhe AA, Ghezzehei TA (2013) A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydr Polym 97(2):253–261. https://doi.org/10.1016/j.carbpol.2013.04.072 10.1016/j.carbpol.2013.04.072

[4]

Alenazi MM, El-Ebidy AM, El-shehaby OA, Seleiman MF, Aldhuwaib KJ, Abdel-Aziz HMM (2024) Chitosan and chitosan nanoparticles differentially alleviate salinity stress in Phaseolus vulgaris L. plants. Plants 13:398. https://doi.org/10.3390/plants13030398 10.3390/plants13030398

[5]

Ali A, Alqurainy F (2006) Activities of antioxidants in plants under environmental stress. In: Motohashi N (ed) The lutein prevention and treatment for diseases. Trans-world Research Network, India, pp 187–256

[6]

Ali EF, El-Shehawi AM, Ibrahim OHM, Abdul-Hafeez EY, Moussa MM, Hassan FAS (2021) A vital role of chitosan nanoparticles in improvisation the drought stress tolerance in Catharanthus roseus (L.) through biochemical and gene expression modulation. Plant Physiol Biochem 161:166–175 10.1016/j.plaphy.2021.02.008

[7]

Ali S, Shakoor A, Ali Q, Chattha MS, El-Sheikh MA, Ali S (2022) Oxidative stress alleviation through enzymatic and non-enzymatic antioxidants and osmoregulators generation in barley (Hordeum vulgare L.) under salt (Nacl) stress by ascorbic acid (ASA). Pak J Bot 54:7–15. https://doi.org/10.30848/PJB2022-1 10.30848/pjb2022-1

[8]

AOAC (Association of official agriculture chemists) (2000) Official methods of analysis, 17th eds. Association of official agriculture chemists, Gaithersburg

[9]

Ashraf M, Iram A (2005) Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora 200:535546 10.1016/j.flora.2005.06.005

[10]

Ashraf M, Shahbaz M, Ali Q (2013) Drought induced modulation in growth and mineral nutrients in canola (Brassica napus L.). Pak J Bot 45:93–98

[11]

Avila RG, Magalhaes PC, Silva EM, Lana UGP, Alvarenga AA, Souza TC (2020) Silicon supplementation improves tolerance to water deficiency in sorghum plants by increasing root system growth and improving photosynthesis. Silicon. https://doi.org/10.1007/s12633-019-00349-5 10.1007/s12633-019-00349-5

[12]

Avila RG, Magalhaes PC, Silva EM, Souza KRD, Campos CN, Alvarenga AA, Souza TC (2021) Application of silicon to irrigated and water deficit sorghum plants increases yield via the regulation of primary, antioxidant, and osmoregulatory metabolism. Agric Water Manag 1016:1016–1070. https://doi.org/10.1016/j.agwat.2021.107004 10.1016/j.agwat.2021.107004

[13]

Avila RG, MagalhAes PC, Vitorino LC, Bessa LA, Souza KR, Queiroz RP, Jakelaitis A, Teixeira MB (2023) Chitosan induces sorghum tolerance to water deficits by positively regulating photosynthesis and the production of primary metabolites, osmoregulators, and antioxidants. J Soil Sci Plant Nutr 23:1156–1172. https://doi.org/10.1007/s42729-022-01111-4 10.1007/s42729-022-01111-4

[14]

Banon SJ, Ochoa J, Franco JA, Alarcon JJ, Sanchez-Blanco MJ (2006) Hardening of oleander seedlings by deficit irrigation and low air humidity. Environ Exp Bot 56:36–43. https://doi.org/10.1016/j.envexpbot.2004.12.004 10.1016/j.envexpbot.2004.12.004

[15]

Basal O, Szabó A (2020) Physiomorphology of soybean as affected by drought stress and nitrogen application. Scientifica. https://doi.org/10.1155/2020/6093836 10.1155/2020/6093836

[16]

Behboudi F, Tahmasebi Sarvestani Z, Kassaee MZ, Modares Sanavi SAM, Sorooshzadeh A, Ahmadi SB (2018) Evaluation of chitosan nanoparticles effects on yield and yield components of barley (Hordeum vulgare L.) under late season drought stress. J Water Environ Nanotechnol 3(1):22–39

[17]

Behboudi F, Tahmasebi-Sarvestani Z, Kassaee MZ, Modarres-Sanavy SAM, Sorooshzadeh A, Mokhtassi-Bidgoli A (2019) Evaluation of chitosan nanoparticles effects with two application methods on wheat under drought stress. J Plant Nutr 42(13):1439–1451 10.1080/01904167.2019.1617308

[18]

Buezo J, Sanz-Saez Á, Moran JF, Soba D, Aranjuelo I, Esteban R (2019) Drought tolerance response of high-yielding soybean varieties to mild drought: physiological and photochemical adjustments. Physiol Plant 166(1):88–104 10.1111/ppl.12864

[19]

Chakraborty M, Hasanuzzaman M, Rahman M, Khan MAR, Bhowmik P, Mahmud NU, Tanveer M, Islam T (2020) Mechanism of plant growth promotion and disease suppression by chitosan biopolymer. Agriculture 10:624 10.3390/agriculture10120624

[20]

Chapman HD, Pratt PF 1978 Method of Analysis for Soil, Plant and Water, California University, Division Agric Sci, Priced Publication, pp 50–169

[21]

Chen JX, Wang XF 2006 Plant physiology experimental guide. High Educ Press Beijing, China 24–25:55–56

[22]

Chibu H, Shibayama H (2001) Effects of chitosan applications on the growth of several crops. In: Uragami T, Kurita K, Fukamizo T (eds) Chitin and chitosan in life science. Yamaguchi, Japan, pp 235–239

[23]

A method for routine measurements of total sugar and starch content in woody plant tissues

P. S. Chow, S. M. Landhausser

Tree Physiology 2004 10.1093/treephys/24.10.1129

[24]

Creelman RA, Mason HG, Bensen RJ, Boyer JS, Mullet JE (1990) Water deficit and abscisic acid causes inhibition of shoots versus root growth in soybean seedlings: analysis of growth, sugar accumulation and gene expression. Plant Physiol 92:205–214 10.1104/pp.92.1.205

[25]

Czekus Z, Iqbal N, Pollak B, Martics A, Ordog A, Poor P (2021) Role of ethylene and light in chitosan-induced local and systemic defence responses of tomato plants. J Plant Physiol 263:153461. https://doi.org/10.1016/j.jplph.2021.153461 10.1016/j.jplph.2021.153461

[26]

Global synthesis of drought effects on cereal, legume, tuber and root crops production: A review

Stefani Daryanto, Lixin Wang, Pierre-Andre Jacinthe

Agricultural Water Management 2020 10.1016/j.agwat.2016.04.022

[27]

Das M, Das SK, Suthar SH (2002) Composition of seed and characteristics of oil from Karingda. Int J Food Sci Technol 37:893–896. https://doi.org/10.1046/j.1365-2621.2002.00638.x 10.1046/j.1365-2621.2002.00638.x

[28]

Dawood MG, Sadak MSh. (2014) Physiological role of glycinebetaine in alleviating the deleterious effects of drought stress on canola plants (Brassica napus L.). Middle East J Agric Res 3(4):943–954

[29]

Dawood MG, Khater MA, El-Awadi ME, Badr NM, Shalaby MAF, Gamal El-Din K, Ahmed MA (2023) Alleviation the deleterious impact of water stress on Sorghum bicolor (L.) plants using biostimulant humic acid. Middle East J Agric Res 12(3):520–535. https://doi.org/10.36632/mejar/2023.12.3.34 10.36632/mejar/2023.12.3.34

[30]

Diatta AA, Fike JH, Battaglia ML, Galbraith J, Baig MB (2020) Effects of biochar on soil fertility and crop productivity in arid regions: a review. Arab J Geosci 13:595 10.1007/s12517-020-05586-2

[31]

Din J, Kans SU, Ali J, Gurmani AR (2011) Physiological and agronomic response of canola varieties to drought stress. J of Anim Plant Sci 21:78–82

[32]

Dos Reis CO, Magalhaes PC, Avila RG, Almeida LG, Rabelo VM, Carvalho DT, Cabral DF, Karam D, de Souza TC (2019) Action of N-Succinyl and N, O-Dicarboxymethyl chitosan derivatives on chlorophyll photosynthesis and fluorescence in drought-sensitive maize. J Plant Growth Regul 38:619–630 10.1007/s00344-018-9877-9

[33]

Du X, Zhang X, Chen X, Jin W, Huang Z, Kong L (2024) Drought stress reduces the photosynthetic source of subtending leaves and the transit sink function of podshells, leading to reduced seed weight in soybean plants. Front Plant Sci 15:1337544. https://doi.org/10.3389/fpls.2024.1337544 10.3389/fpls.2024.1337544

[34]

Multiple Range and Multiple F Tests

David B. Duncan

Biometrics 1955 10.2307/3001478

[35]

El F, AmeranyRhazi M, Balcke G, Wahbi S, Meddich A, Taourirte M, Hause B (2022) The effect of chitosan on plant physiology, wound response, and fruit quality of tomato. Polymers 14:5006. https://doi.org/10.3390/polym14225006 10.3390/polym14225006

[36]

El-Awadi ME, Sadak MSh., Dawood MG (2021) Comparative effect of potassium and banana peel in alleviating the deleterious effect of water deficit on soybean plants. J Mater Environ Sci 12(7):929–943

[37]

Elewa TA, Sadak MSh., Dawood MG (2017) Improving drought tolerance of quinoa plant by foliar treatment of trehalose. Agric Eng Int CIGR J 19(5):245–254

[38]

El-Zamily EAM, EL-Shafey AS, Dawood MG, El-Awadi ME (2024) Investigating the physiological roles of bulk chitosan and nano-chitosan in growth, flowering, yield attributes of Brassica napus L. and in nutrient delivery in sandy soils. Middle East J Appl Sci 14(1):161–179. https://doi.org/10.36632/mejas/2024.14.1.11 10.36632/mejas/2024.14.1.11

[39]

Gene expression for secondary metabolite biosynthesis in hop (Humulus lupulus L.) leaf lupulin glands exposed to heat and low-water stress

Renée L. Eriksen, Lillian K. Padgitt-Cobb, M. Shaun Townsend et al.

Scientific Reports 2021 10.1038/s41598-021-84691-y

[40]

Esmaeeli M, Roozbahani A, Daneshian J (2024) Combined effects chitosan and genotype on agronomic, physiologic, and biochemical characteristics of soybean under drought stress conditions. S Afr J Bot 174:678–685. https://doi.org/10.1016/j.sajb.2024.09.036 10.1016/j.sajb.2024.09.036

[41]

Faqir Y, Ma J, Chai Y (2021) Chitosan in modern agriculture production. Plant Soil Environ 67(12):679–699. https://doi.org/10.17221/332/2021-PSE 10.17221/332/2021-pse

[42]

Fazeli F, Ghorbanli M, Niknam V (2007) Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars. Biol Plant 51(1):98–103 10.1007/s10535-007-0020-1

[43]

García-García AL, García-Machado FJ, Borges AA, Morales-Sierra S, Boto A, Jiménez-Arias D (2020) Pure organic active compounds against abiotic stress: a biostimulant overview. Front Plant Sci 11:575829. https://doi.org/10.3389/fpls.2020.575829 10.3389/fpls.2020.575829

[44]

Garcı́a-Garcı́a AL, Matos AR, Feijão E, Cruz de Carvalho R, Boto A, Marques da Silva J, Jime´ nez-Arias D (2023) The use of chitosan oligosaccharide to improve artemisinin yield in well watered and drought-stressed plants. Front Plant Sci 14:1200898. https://doi.org/10.3389/fpls.2023.1200898 10.3389/fpls.2023.1200898

[45]

Ge TD, Sui FG, Bai LP, Yin-yan LU, Guang-sheng Z (2006) Effects of water stress on the protective enzyme activities and lipid per- oxidation in roots and leaves of summer maize. Agric Sci China 5(4):291–298. https://doi.org/10.1016/S1671-2927(06)60052-7 10.1016/s1671-2927(06)60052-7

[46]

Geng W, Zhou L, Muhammad JH, Yan P (2020) Chitosan regulates metabolic balance, polyamine accumulation, and Na+ transport contributing to salt tolerance in creeping bentgrass Geng. BMC Plant Biol 20:506 10.1186/s12870-020-02720-w

[47]

Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants

Sarvajeet Singh Gill, Narendra Tuteja

Plant Physiology and Biochemistry 2010 10.1016/j.plaphy.2010.08.016

[48]

Gonzalez MB, Guzman R, Rudkyk E, Romano M, Molina AA (2003) Spectrophotometric determination of phenolic compounds in propolis. Lat Am J Pharm 22(3):243–248

[49]

Guan Y, Hu J, Wang X, Shao C (2009) Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. J Zhejiang Univ Sci B 10:427–433. https://doi.org/10.1631/jzus.b0820373 10.1631/jzus.b0820373

[50]

Gusmiaty M, Restu A, Payangan RY (2019) Production of IAA (Indole Acetic Acid) of the rhizosphere fungus in the suren community forest stand. IOP Conf Ser Earth Environ Sci 343:012058 10.1088/1755-1315/343/1/012058

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Published: Apr 08, 2026
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Authors

M

Mervat Shamoon Sadak

M

Mohamed El-sayed El-Awadi

M

Mona Gergis Dawood

Y

Yasser Refaai Abdel-Baky

Cite This Article

Mervat Shamoon Sadak, Mohamed El-sayed El-Awadi, Mona Gergis Dawood, et al. (2026). Efficiency of chitosan to induce the immune defense system of soybean plant to drought stress. Vegetos. https://doi.org/10.1007/s42535-026-01643-5

Efficiency of chitosan to induce the immune defense system of soybean plant to drought stress

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