Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity

Yelena Lyubarskaya; Damian Houde; James Woodard; David Murphy; Rohin Mhatre

doi:10.1016/j.ab.2005.10.003

journal article Jan 01, 2006

Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity

Yelena Lyubarskaya Damian Houde James Woodard David Murphy

Rohin Mhatre

Analytical Biochemistry Vol. 348 No. 1 pp. 24-39 · Elsevier BV

View at Publisher Save 10.1016/j.ab.2005.10.003

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References

25

[1]

Zhang "Separation and characterization of a monoclonal IgG2 antibody by cation exchange chromatography" Bio Process J. (2003)

[2]

Identification of multiple sources of charge heterogeneity in a recombinant antibody

Reed J. Harris, Bruce Kabakoff, Frank D. Macchi et al.

Journal of Chromatography B: Biomedical Sciences a... 2001 10.1016/s0378-4347(00)00548-x

[3]

Determination of the Origin of Charge Heterogeneity in a Murine Monoclonal Antibody

Melissa Perkins, Rolf THEILER, Susan Lunte et al.

Pharmaceutical Research 2000 10.1023/a:1026461830617

[4]

Tsai "Origin of the isoelectric heterogeneity of monoclonal immunoglobulin h1B4" Pharm. Res. (1993) 10.1023/a:1018912417607

[5]

Identification of Sites of Degradation in a Therapeutic Monoclonal Antibody by Peptide Mapping

Daniel J. Kroon, Alysia Baldwin-Ferro, Praful Lalan

Pharmaceutical Research 1992 10.1023/a:1015894409623

[6]

Harris "Assessing genetic heterogeneity in production cell lines: detection by peptide mapping of a low level Tyr to Gln sequence variant in a recombinant antibody" Biotechnology (1993)

[7]

Papac "A high-throughput microscale method to release N-linked oligosaccharides from glycoproteins for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis" Glycobiology (1998) 10.1093/glycob/8.5.445

[8]

Lapolla "A study on in vitro glycation processes by matrix-assisted laser desorption ionization mass spectrometry" Biochim. Biophys. Acta (1993) 10.1016/0925-4439(93)90118-k

[9]

Alexander "Monitoring of IgG antibody thermal stability by micellar electrokinetic capillary chromatography and matrix-assisted laser desorption/ionization mass spectrometry" Anal. Chem. (1995) 10.1021/ac00116a002

[10]

King "Expression, purification, and characterization of a mouse–human chimeric antibody and chimeric Fab′ fragment" Biochem. J. (1992) 10.1042/bj2810317

[11]

Kannan "Rapid analytical tryptic mapping of a recombinant chimeric monoclonal antibody and method validation challenges" J. Pharm. Biomed. Anal. (1997) 10.1016/s0731-7085(97)00179-9

[12]

Hunt "Capillary electrophoresis sodium dodecyl sulfate nongel sieving analysis of a therapeutic recombinant monoclonal antibody: a biotechnology perspective" Anal. Chem. (1999) 10.1021/ac981209m

[13]

Cacia "Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to human IgE: identification and effect on binding affinity" Biochemistry (1996) 10.1021/bi951526c

[14]

Lewis "Characterization of humanized anti-TAC, an antibody directed against the interleukin 2 receptor, using electrospray ionization mass spectrometry by direct infusion, LC/MS, and MS/MS" Anal. Chem. (1994) 10.1021/ac00077a003

[15]

Roberts "An integrated strategy for structural characterization of the protein and carbohydrate components of monoclonal antibodies: application to anti-respiratory syncytial virus mAb" Anal. Chem. (1995) 10.1021/ac00116a001

[16]

Ashton "Mass spectrometry of the humanized monoclonal antibody campath 1H" Anal. Chem. (1995) 10.1021/ac00101a008

[17]

Sunday "Structure characterization of a recombinant monoclonal antibody with one- and two-dimensional high performance liquid chromatography with on-line electrospray ionization mass spectrometry" Bioprocess J. (2003) 10.12665/j23.sunday

[18]

Santora "Determination of monoclonal antibodies and noncovalent antigen TNFα trimer using Q-TOF mass spectrometry" Spectroscopy (2002)

[19]

Dillon "Development of an analytical reversed-phase high-performance liquid chromatography–electrospray ionization mass spectrometry method for characterization of recombinant antibodies" J. Chromatogr. A (2004) 10.1016/s0021-9673(04)01410-4

[20]

Dore-Duffy "Expression of endothelial cell activation antigens in microvessels from patients with multiple sclerosis" Adv. Exp. Med. Biol. (1993) 10.1007/978-1-4615-2920-0_38

[21]

Briskin "Human mucosal addressin cell adhesion molecule-1 is preferentially expressed in intestinal tract and associated lymphoid tissue" Am. J. Pathol. (1997)

[22]

Bigge "Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid" Anal. Biochem. (1995) 10.1006/abio.1995.1468

[23]

Raju "Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics" Glycobiology (2000) 10.1093/glycob/10.5.477

[24]

Awade "Pyrrolidone carboxyl peptidase (PCP): an enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptides and pGlu-proteins" Proteins (1994) 10.1002/prot.340200106

[25]

Krishna "Posttranslational modifications" (1998)

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Published: Jan 01, 2006
Vol/Issue: 348(1)
Pages: 24-39
License: View

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Cite This Article

Yelena Lyubarskaya, Damian Houde, James Woodard, et al. (2006). Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity. Analytical Biochemistry, 348(1), 24-39. https://doi.org/10.1016/j.ab.2005.10.003

Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity

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