Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity

Renchao Chen; Xiaoji Wu; Lan Jiang

doi:10.1016/j.celrep.2017.03.004

journal article Open Access Mar 01, 2017

Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity

Renchao Chen Xiaoji Wu Lan Jiang

Cell Reports Vol. 18 No. 13 pp. 3227-3241 · Elsevier BV

View at Publisher Save 10.1016/j.celrep.2017.03.004

Topics

No keywords indexed for this article. Browse by subject →

References

46

[1]

Bolborea "Hypothalamic tanycytes: potential roles in the control of feeding and energy balance" Trends Neurosci. (2013) 10.1016/j.tins.2012.12.008

[2]

Isolation and culture of adult neurons and neurospheres

Gregory J Brewer, John R Torricelli

Nature Protocols 2007 10.1038/nprot.2007.207

[3]

Brown "Physiological regulation of magnocellular neurosecretory cell activity: integration of intrinsic, local and afferent mechanisms" J. Neuroendocrinol. (2013) 10.1111/jne.12051

[4]

Chen "The oligodendrocyte-specific G protein-coupled receptor GPR17 is a cell-intrinsic timer of myelination" Nat. Neurosci. (2009) 10.1038/nn.2410

[5]

Denton "Hypothalamic integration of body fluid regulation" Proc. Natl. Acad. Sci. USA (1996) 10.1073/pnas.93.14.7397

[6]

Dibner "The mammalian circadian timing system: organization and coordination of central and peripheral clocks" Annu. Rev. Physiol. (2010) 10.1146/annurev-physiol-021909-135821

[7]

Elmquist "From lesions to leptin: hypothalamic control of food intake and body weight" Neuron (1999) 10.1016/s0896-6273(00)81084-3

[8]

Emery "Transcriptional and epigenetic regulation of oligodendrocyte development and myelination in the central nervous system" Cold Spring Harb. Perspect. Biol. (2015) 10.1101/cshperspect.a020461

[9]

Gokce "Cellular taxonomy of the mouse striatum as revealed by single-cell RNA-seq" Cell Rep. (2016) 10.1016/j.celrep.2016.06.059

[10]

A gene expression atlas of the central nervous system based on bacterial artificial chromosomes

Shiaoching Gong, Chen Zheng, Martin L. Doughty et al.

Nature 2003 10.1038/nature02033

[11]

Goodman "Hypothalamic tanycytes-masters and servants of metabolic, neuroendocrine, and neurogenic functions" Front. Neurosci. (2015) 10.3389/fnins.2015.00387

[12]

Greig "Molecular logic of neocortical projection neuron specification, development and diversity" Nat. Rev. Neurosci. (2013) 10.1038/nrn3586

[13]

Harmar "The VPAC(2) receptor is essential for circadian function in the mouse suprachiasmatic nuclei" Cell (2002) 10.1016/s0092-8674(02)00736-5

[14]

Henry "Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss" eLife (2015) 10.7554/elife.09800

[15]

Jiang "Principles of connectivity among morphologically defined cell types in adult neocortex" Science (2015) 10.1126/science.aac9462

[16]

Kiselev "SC3 - consensus clustering of single-cell RNA-seq data" bioRxiv (2016)

[17]

Klein "Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells" Cell (2015) 10.1016/j.cell.2015.04.044

[18]

Molecular Profiling of Activated Neurons by Phosphorylated Ribosome Capture

Zachary A. Knight, Keith Tan, Kivanc Birsoy et al.

Cell 2012 10.1016/j.cell.2012.10.039

[19]

Krashes "An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger" Nature (2014) 10.1038/nature12956

[20]

Lake "Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain" Science (2016) 10.1126/science.aaf1204

[21]

Lee "Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche" Nat. Neurosci. (2012) 10.1038/nn.3079

[22]

Lee "Neuromedin s-producing neurons act as essential pacemakers in the suprachiasmatic nucleus to couple clock neurons and dictate circadian rhythms" Neuron (2015) 10.1016/j.neuron.2015.02.006

[23]

Lein "Genome-wide atlas of gene expression in the adult mouse brain" Nature (2007) 10.1038/nature05453

[24]

Ludwig "Dendritic peptide release and peptide-dependent behaviours" Nat. Rev. Neurosci. (2006) 10.1038/nrn1845

[25]

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

Evan Z. Macosko, Anindita Basu, Rahul Satija et al.

Cell 2015 10.1016/j.cell.2015.05.002

[26]

Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system

Sueli Marques, Amit Zeisel, Simone Codeluppi et al.

Science 2016 10.1126/science.aaf6463

[27]

Mathew "Regional analysis of the ependyma of the third ventricle of rat by light and electron microscopy" Anat. Histol. Embryol. (2008) 10.1111/j.1439-0264.2007.00786.x

[28]

Mayerl "Transporters MCT8 and OATP1C1 maintain murine brain thyroid hormone homeostasis" J. Clin. Invest. (2014) 10.1172/jci70324

[29]

Miranda-Angulo "Rax regulates hypothalamic tanycyte differentiation and barrier function in mice" J. Comp. Neurol. (2014) 10.1002/cne.23451

[30]

Murray "60 YEARS OF NEUROENDOCRINOLOGY: The hypothalamo-GH axis: the past 60 years" J. Endocrinol. (2015) 10.1530/joe-15-0120

[31]

Navarro "Neuroendocrine control by kisspeptins: role in metabolic regulation of fertility" Nat. Rev. Endocrinol. (2011) 10.1038/nrendo.2011.147

[32]

Puelles "A new scenario of hypothalamic organization: rationale of new hypotheses introduced in the updated prosomeric model" Front. Neuroanat. (2015) 10.3389/fnana.2015.00027

[33]

Robins "α-Tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors" Nat. Commun. (2013) 10.1038/ncomms3049

[34]

Salvatierra "The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation" J. Neurosci. (2014) 10.1523/jneurosci.1711-14.2014

[35]

Samanta "Interactions between ID and OLIG proteins mediate the inhibitory effects of BMP4 on oligodendroglial differentiation" Development (2004) 10.1242/dev.01273

[36]

Schwartz "Central nervous system control of food intake" Nature (2000) 10.1038/35007534

[37]

Shimogori "A genomic atlas of mouse hypothalamic development" Nat. Neurosci. (2010) 10.1038/nn.2545

[38]

Adult mouse cortical cell taxonomy revealed by single cell transcriptomics

Bosiljka Tasic, Vilas Menon, Thuc Nghi Nguyen et al.

Nature Neuroscience 2016 10.1038/nn.4216

[39]

Toledo-Rodriguez "Correlation maps allow neuronal electrical properties to be predicted from single-cell gene expression profiles in rat neocortex" Cereb. Cortex (2004) 10.1093/cercor/bhh092

[40]

Tong "Synaptic release of GABA by AgRP neurons is required for normal regulation of energy balance" Nat. Neurosci. (2008) 10.1038/nn.2167

[41]

The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells

Cole Trapnell, Davide Cacchiarelli, Jonna Grimsby et al.

Nature Biotechnology 2014 10.1038/nbt.2859

[42]

VanDunk "Development, maturation, and necessity of transcription factors in the mouse suprachiasmatic nucleus" J. Neurosci. (2011) 10.1523/jneurosci.5385-10.2011

[43]

Wahab "The involvement of gonadotropin inhibitory hormone and kisspeptin in the metabolic regulation of reproduction" J. Endocrinol. (2015) 10.1530/joe-14-0688

[44]

Wu "Galanin neurons in the medial preoptic area govern parental behaviour" Nature (2014) 10.1038/nature13307

[45]

Ypsilanti "Transcriptional and epigenetic mechanisms of early cortical development: An examination of how Pax6 coordinates cortical development" J. Comp. Neurol. (2016) 10.1002/cne.23866

[46]

Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

Amit Zeisel, Ana B. Muñoz-Manchado, Simone Codeluppi et al.

Science 2015 10.1126/science.aaa1934

Cited By

572

Modeling hypothalamic pathophysiology in vitro for metabolic, circadian, and sleep disorders

Helena Leal, Catarina Carvalhas-Almeida · 2024

Trends in Endocrinology & Metab...

Region-specific transcriptomic responses to obesity and diabetes in macaque hypothalamus

Ying Lei, Xian Liang · 2024

Cell Metabolism

A subcortical feeding circuit linking an interoceptive node to jaw movement

Christin Kosse, Jessica Ivanov · 2024

Nature

Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus

Brian R. Herb, Hannah J. Glover · 2023

Science Advances

Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis

Qiang Yu, Igor Gamayun · 2023

Nature Communications

Transcriptional profiling of Kiss1 neurons from arcuate and rostral periventricular hypothalamic regions in female mice

Stephen Manchishi, Malwina Prater · 2023

Reproduction

Developmental single-cell transcriptomics of hypothalamic POMC neurons reveal the genetic trajectories of multiple neuropeptidergic phenotypes

Hui Yu, Marcelo Rubinstein · 2022

eLife

Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypes

Behzad Yaghmaeian Salmani, Brad Balderson · 2022

Development

Deciphering the spatial-temporal transcriptional landscape of human hypothalamus development

Xin Zhou, Yufeng Lu · 2022

Cell Stem Cell

Ontogenetic rules for the molecular diversification of hypothalamic neurons

Marco Benevento, Tomas Hökfelt · 2022

Nature Reviews Neuroscience

Transcriptome profiling of kisspeptin neurons from the mouse arcuate nucleus reveals new mechanisms in estrogenic control of fertility

Balázs Göcz, Éva Rumpler · 2022

Proceedings of the National Academy...

Tany-Seq: Integrated Analysis of the Mouse Tanycyte Transcriptome

Andrew I. Sullivan, Matthew J. Potthoff · 2022

Cells

Cascade diversification directs generation of neuronal diversity in the hypothalamus

Yu-hong Zhang, Mingrui Xu · 2021

Cell Stem Cell

Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations

Alison H Affinati, Paul V Sabatini · 2021

eLife

Decoding molecular and cellular heterogeneity of mouse nucleus accumbens

Renchao Chen, Timothy R. Blosser · 2021

Nature Neuroscience

Identification of novel GPCR partners of the central melanocortin signaling

Yunpeng Li, Xiaozhu Wang · 2021

Molecular Metabolism

Development of the GABAergic and glutamatergic neurons of the lateral hypothalamus

Xunlei Zhou, Pierre-Yves Risold · 2021

Journal of Chemical Neuroanatomy

Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators

Christian Huisman, Hyeyoung Cho · 2019

Nature Communications

Single-cell transcriptomic analysis of the lateral hypothalamic area reveals molecularly distinct populations of inhibitory and excitatory neurons

Laura E. Mickelsen, Mohan Bolisetty · 2019

Nature Neuroscience

Multimodal Analysis of Cell Types in a Hypothalamic Node Controlling Social Behavior

Dong-Wook Kim, Zizhen Yao · 2019

Cell

Metrics

572

Citations

46

References

Details

Published: Mar 01, 2017
Vol/Issue: 18(13)
Pages: 3227-3241
License: View

Authors

R

Renchao Chen

Vizgen, Cambridge, MA.

Laser Micro/Nano Fabrication Laboratory School of Mechanical Engineering Beijing Institute of Technology Beijing 100081 China

Funding

NIH Award: U01 MH105960

Cite This Article

Renchao Chen, Xiaoji Wu, Lan Jiang (2017). Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity. Cell Reports, 18(13), 3227-3241. https://doi.org/10.1016/j.celrep.2017.03.004

Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity

You May Also Like