A consensus introduction to serum replacements and serum-free media for cellular therapies

Ohad Karnieli; Oryan Makler Friedner; Julie G. Allickson; Nan Zhang; Sunghoon Jung; David Fiorentini; Eytan Abraham; Shannon S. Eaker; tan Kah Yong; Allan Chan; Sarah Griffiths; Amy K. Wehn; Steve Oh

doi:10.1016/j.jcyt.2016.11.011

journal article Feb 01, 2017

A consensus introduction to serum replacements and serum-free media for cellular therapies

Ohad Karnieli Oryan Makler Friedner Julie G. Allickson Nan Zhang

Sunghoon Jung

David Fiorentini Eytan Abraham Shannon S. Eaker tan Kah Yong Allan Chan Sarah Griffiths

Amy K. Wehn Steve Oh

Cytotherapy Vol. 19 No. 2 pp. 155-169 · Elsevier BV

View at Publisher Save 10.1016/j.jcyt.2016.11.011

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References

55

[1]

Culme-Seymour "A decade of cell therapy clinical trials (2000–2010)" Regen Med (2012) 10.2217/rme.12.45

[2]

Brindley "Peak serum: implications of serum supply for cell therapy manufacturing" Regen Med (2012) 10.2217/rme.11.112

[3]

Davie (2014)

[4]

Mendicino "MSC-based product characterization for clinical trials: an FDA perspective" Cell Stem Cell (2014) 10.1016/j.stem.2014.01.013

[5]

Siegel "Fetal bovine serum: the impact of geography" Bioprocess J (2013) 10.12665/j123.siegel

[6]

Jochems "The use of fetal bovine serum: ethical or scientific problem?" Altern Lab Anim (2002) 10.1177/026119290203000208

[7]

Zuliani "Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag: interests for adoptive immunotherapy" J Transl Med (2011) 10.1186/1479-5876-9-63

[8]

da Silva (2014)

[9]

Gstraunthaler "Alternatives to the use of fetal bovine serum: serum-free cell culture" ALTEX (2003)

[10]

Gottipamula "Serum-free media for the production of human mesenchymal stromal cells: a review" Cell Prolif (2013) 10.1111/cpr.12063

[11]

Gstraunthaler "A plea to reduce or replace fetal bovine serum in cell culture media" Cytotechnology (2013) 10.1007/s10616-013-9633-8

[12]

Boone "Quality control studies on fetal bovine serum used in tissue culture" In Vitro (1971) 10.1007/bf02617963

[13]

Alden "Porcine platelet lysate as a supplement for animal cell culture" Cytotechnology (2007) 10.1007/s10616-007-9097-9

[14]

Holmovist (1989)

[15]

World Health Organization (2011)

[16]

Munirah "Autologous versus pooled human serum for articular chondrocyte growth" J Orthop Surg (Hong Kong) (2008) 10.1177/230949900801600219

[17]

Hankey "Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum" Acta Orthop Scand (2001) 10.1080/000164701753542069

[18]

Thuresson "Apparent half-lives of hepta- to decabrominated diphenyl ethers in human serum as determined in occupationally exposed workers" Environ Health Perspect (2006) 10.1289/ehp.8350

[19]

Yamamoto "Effects of autologous serum on osteoblastic differentiation in human bone marrow cells" J Med Dent Sci (2003)

[20]

Clavreul "Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy" In Vitro Cell Dev Biol Anim (2009) 10.1007/s11626-009-9215-4

[21]

Pandolfino "Comparison of three culture media for the establishment of melanoma cell lines" Cytotechnology (2010) 10.1007/s10616-010-9286-9

[22]

Jung "Identification of growth and attachment factors for the serum-free isolation and expansion of human mesenchymal stromal cells" Cytotherapy (2010) 10.3109/14653249.2010.495113

[23]

Jung "Ex vivo expansion of human mesenchymal stem cells in defined serum-free media" Stem Cells Int (2012) 10.1155/2012/123030

[24]

Smith "Ex vivo expansion of human T cells for adoptive immunotherapy using the novel Xeno-free CTS Immune Cell Serum Replacement" Clin Transl Immunology (2015) 10.1038/cti.2014.31

[25]

Wang "Efficient and scalable expansion of human pluripotent stem cells under clinically compliant settings: a view in 2013" Ann Biomed Eng (2014) 10.1007/s10439-013-0921-4

[26]

Chase "A novel serum-free medium for the expansion of human mesenchymal stem cells" Stem Cell Res Ther (2010) 10.1186/scrt8

[27]

Fischer "Pulmonary passage is a major obstacle for intravenous stem cell delivery: the pulmonary first-pass effect" Stem Cells Dev (2009) 10.1089/scd.2008.0253

[28]

Lee "Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6" Cell Stem Cell (2009) 10.1016/j.stem.2009.05.003

[29]

Schrepfer "Stem cell transplantation: the lung barrier" Transplant Proc (2007) 10.1016/j.transproceed.2006.12.019

[30]

Bartosh "Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties" Proc Natl Acad Sci USA (2010) 10.1073/pnas.1008117107

[31]

Wu "Serum-free media and the immunoregulatory properties of mesenchymal stem cells in vivo and in vitro" Cell Physiol Biochem (2014) 10.1159/000358635

[32]

Doucet "Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications" J Cell Physiol (2005) 10.1002/jcp.20391

[33]

King "Characterization and partial purification of an endothelial cell growth factor from human platelets" J Clin Invest (1984) 10.1172/jci111224

[34]

Chen "The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars" Biomaterials (2012) 10.1016/j.biomaterials.2012.03.057

[35]

Atashi "Autologous platelet-rich plasma: a biological supplement to enhance adipose-derived mesenchymal stem cell expansion" Tissue Eng Part C Methods (2015) 10.1089/ten.tec.2014.0206

[36]

Kocaoemer "Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue" Stem Cells (2007) 10.1634/stemcells.2006-0627

[37]

Cantisan Bohorquez "Immunological monitoring strategies for cytomegalovirus infection. Immune-based therapies" Enferm Infecc Microbiol Clin (2011)

[38]

Human mesenchymal stem cell culture: rapid and efficient isolation and expansion in a defined serum-free medium

Sunghoon Jung, Arindom Sen, Lawrence Rosenberg et al.

Journal of Tissue Engineering and Regenerative Med... 2012 10.1002/term.441

[39]

Dolley-Sonneville "Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions" PLoS ONE (2013) 10.1371/journal.pone.0070263

[40]

Hervy "Long term expansion of bone marrow-derived hMSCs on novel synthetic microcarriers in xeno-free, defined conditions" PLoS ONE (2014) 10.1371/journal.pone.0092120

[41]

Ishikawa "Adoptive cell transfer therapy for malignant gliomas" Adv Exp Med Biol (2012) 10.1007/978-1-4614-3146-6_9

[42]

Guillot "Human first-trimester fetal MSC express pluripotency markers and grow faster and have longer telomeres than adult MSC" Stem Cells (2007) 10.1634/stemcells.2006-0208

[43]

Wegmeyer "Mesenchymal stromal cell characteristics vary depending on their origin" Stem Cells Dev (2013) 10.1089/scd.2013.0016

[44]

Laport "Adoptive immunotherapy with cytokine-induced killer cells for patients with relapsed hematologic malignancies after allogeneic hematopoietic cell transplantation" Biol Blood Marrow Transplant (2011) 10.1016/j.bbmt.2011.05.012

[45]

Papa "Clinical evaluation of ErbB-targeted CAR T-cells, following intracavity delivery in patients with ErbB-expressing solid tumors" Methods Mol Biol (2015) 10.1007/978-1-4939-2727-2_21

[46]

Park "Treating cancer with genetically engineered T cells" Trends Biotechnol (2011) 10.1016/j.tibtech.2011.04.009

[47]

Ozturk "Examination of serum and bovine serum albumin as shear protective agents in agitated cultures of hybridoma cells" J Biotechnol (1991) 10.1016/0168-1656(91)90232-k

[48]

Murhammer "Sparged animal cell bioreactors: mechanism of cell damage and Pluronic F-68 protection" Biotechnol Prog (1990) 10.1021/bp00005a012

[49]

Thompson "Umbilical cord blood graft engineering: challenges and opportunities" Bone Marrow Transplant (2015) 10.1038/bmt.2015.97

[50]

Pagliara "Cytotoxic T lymphocytes for the treatment of viral infections and posttransplant lymphoproliferative disorders in transplant recipients" Curr Opin Infect Dis (2012) 10.1097/qco.0b013e3283551dd3

Showing 50 of 55 references

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Metrics

184

Citations

55

References

Details

Published: Feb 01, 2017
Vol/Issue: 19(2)
Pages: 155-169
License: View

Authors

O

Ohad Karnieli

O

Oryan Makler Friedner

Department of Life Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK

Cite This Article

Ohad Karnieli, Oryan Makler Friedner, Julie G. Allickson, et al. (2017). A consensus introduction to serum replacements and serum-free media for cellular therapies. Cytotherapy, 19(2), 155-169. https://doi.org/10.1016/j.jcyt.2016.11.011

A consensus introduction to serum replacements and serum-free media for cellular therapies

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