Emergence of Anisotropic Subcompartments via Coassembly of Hierarchically Ordered G-Quadruplexes and Fluid Polylysine in Droplet-Based Compartments

Hiroka Sugai; Shunsuke Tomita; Mariko Toyoda; Kota Watanuki; Mao Fukuyama; Takashi Kajitani; Kazushi Kinbara

doi:10.1021/acsnano.5c21437

journal article Open Access Mar 26, 2026

Emergence of Anisotropic Subcompartments via Coassembly of Hierarchically Ordered G-Quadruplexes and Fluid Polylysine in Droplet-Based Compartments

Hiroka Sugai

Shunsuke Tomita

Mariko Toyoda Kota Watanuki Mao Fukuyama

Takashi Kajitani Kazushi Kinbara

ACS Nano Vol. 20 No. 13 pp. 10520-10531 · American Chemical Society (ACS)

View at Publisher Save 10.1021/acsnano.5c21437

Topics

No keywords indexed for this article. Browse by subject →

References

78

[1]

Liquid-Liquid Phase Separation in Biology

Anthony A. Hyman, Christoph A. Weber, Frank Jülicher

Annual Review of Cell and Developmental Biology 10.1146/annurev-cellbio-100913-013325

[2]

Liquid phase condensation in cell physiology and disease

Yongdae Shin, Clifford P. Brangwynne

Science 10.1126/science.aaf4382

[3]

A guide to membraneless organelles and their various roles in gene regulation

Tetsuro Hirose, Kensuke Ninomiya, Shinichi Nakagawa et al.

Nature Reviews Molecular Cell Biology 10.1038/s41580-022-00558-8

[4]

10.1126/science.1172046

[5]

The nucleolus as a multiphase liquid condensate

Denis L. J. Lafontaine, Joshua A. Riback, Rümeyza Bascetin et al.

Nature Reviews Molecular Cell Biology 10.1038/s41580-020-0272-6

[6]

10.1021/acs.chemrev.2c00608

[7]

10.1038/s41580-022-00508-4

[8]

10.1038/s41580-021-00399-x

[9]

Amyloid-type Protein Aggregation and Prion-like Properties of Amyloids

Dieter Willbold, Birgit Strodel, Gunnar F. Schröder et al.

Chemical Reviews 10.1021/acs.chemrev.1c00196

[10]

Membrane lipids: where they are and how they behave

Gerrit van Meer, Dennis R. Voelker, Gerald W. Feigenson

Nature Reviews Molecular Cell Biology 10.1038/nrm2330

[11]

10.1038/s41580-022-00524-4

[12]

10.1088/1361-6633/adb441

[13]

10.1016/j.cell.2025.04.039

[14]

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

Avinash Patel, Hyun O. Lee, Louise Jawerth et al.

Cell 10.1016/j.cell.2015.07.047

[15]

10.1038/s41557-020-0465-9

[16]

Tau protein liquid–liquid phase separation can initiate tau aggregation

Susanne Wegmann, Bahareh Eftekharzadeh, Katharina Tepper et al.

The EMBO Journal 10.15252/embj.201798049

[17]

10.1016/j.cell.2017.05.028

[18]

10.1038/s41467-019-14087-0

[19]

Liquid–liquid crystalline phase separation in biomolecular solutions

Tommaso P. Fraccia, Giuliano Zanchetta

Current Opinion in Colloid & Interface Science 10.1016/j.cocis.2021.101500

[20]

Liquid–Liquid Phase Separation in Crowded Environments

Alain A. M. André, Evan Spruijt

International Journal of Molecular Sciences 10.3390/ijms21165908

[21]

10.1016/j.bpj.2018.09.022

[22]

10.1021/acsnano.0c05083

[23]

10.1126/science.1143826

[24]

10.1021/acsnano.6b03622

[25]

10.1021/acs.bioconjchem.1c00302

[26]

10.1038/s41467-023-38163-8

[27]

10.1016/j.tibs.2023.06.007

[28]

10.1016/j.tig.2022.10.006

[29]

The regulation and functions of DNA and RNA G-quadruplexes

Dhaval Varshney, Jochen Spiegel, Katherine Zyner et al.

Nature Reviews Molecular Cell Biology 10.1038/s41580-020-0236-x

[30]

10.1002/1873-3468.13547

[31]

10.1021/ja00198a057

[32]

10.1080/15421400390221178

[33]

10.1021/acsnano.4c09691

[34]

10.1021/ja061267m

[35]

10.1021/jacs.1c03447

[36]

10.1021/jacs.1c03627

[37]

10.1016/j.jbc.2021.101284

[38]

10.1039/d1cc01479j

[39]

10.1039/d2cc04366a

[40]

10.1016/j.cplett.2023.140634

[41]

10.1021/jacs.2c11362

[42]

10.1021/jacsau.3c00106

[43]

10.1038/s41467-024-45353-5

[44]

10.1021/jacsau.4c00690

[45]

10.1016/j.jbc.2024.107971

[46]

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

Marina Feric, Nilesh Vaidya, Tyler S. Harmon et al.

Cell 10.1016/j.cell.2016.04.047

[47]

10.1002/wrna.1514

[48]

10.1021/jacs.9b03689

[49]

10.1021/acs.biomac.2c01148

[50]

10.1016/s0006-3495(98)77824-5

Showing 50 of 78 references

Metrics

0

Citations

78

References

Details

Published: Mar 26, 2026
Vol/Issue: 20(13)
Pages: 10520-10531
License: View

Authors

H

Hiroka Sugai

Research Center for Autonomous Systems Materialogy (ASMat), Institute of Integrated Research, Institute of Science Tokyo, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan; Japan Science and Technology Agency (JST); Precursory Research for Embryonic Science and Technology (PRESTO)

S

Shunsuke Tomita

Health and Medical Research Institute; National Institute of Advanced Industrial Science and Technology (AIST); School of Integrative and Global Majors; University of Tsukuba

M

Mariko Toyoda

Research Center for Autonomous Systems Materialogy (ASMat), Institute of Integrated Research, Institute of Science Tokyo, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

K

Kota Watanuki

School of Life Science and Technology, Institute of Science Tokyo, 4259, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

M

Mao Fukuyama

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira, Sendai, Miyagi 980-8577, Japan

T

Takashi Kajitani

Core Facility Center, Research Infrastructure Management Center, Institute of Science Tokyo, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

K

Kazushi Kinbara

Research Center for Autonomous Systems Materialogy (ASMat), Institute of Integrated Research, Institute of Science Tokyo, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan; School of Life Science and Technology, Institute of Science Tokyo, 4259, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

Funding

Japan Society for the Promotion of Science Award: JP22K14777

Precursory Research for Embryonic Science and Technology Award: JPMJPR22E5

Center for Fundamental Research

Yoshinori Ohsumi Award for Fundamental Research

Cite This Article

Hiroka Sugai, Shunsuke Tomita, Mariko Toyoda, et al. (2026). Emergence of Anisotropic Subcompartments via Coassembly of Hierarchically Ordered G-Quadruplexes and Fluid Polylysine in Droplet-Based Compartments. ACS Nano, 20(13), 10520-10531. https://doi.org/10.1021/acsnano.5c21437

Emergence of Anisotropic Subcompartments via Coassembly of Hierarchically Ordered G-Quadruplexes and Fluid Polylysine in Droplet-Based Compartments

You May Also Like