A Scalable 3D High-Content Imaging Protocol for Measuring a Drug Induced DNA Damage Response Using Immunofluorescent Subnuclear γH2AX Spots in Patient Derived Ovarian Cancer Organoids

Hakan KELEŞ; Christopher A. Schofield; Helena Rannikmae; Erin Elizabeth Edwards; Lisa Mohamet

doi:10.1021/acsptsci.2c00200

journal article Dec 12, 2022

A Scalable 3D High-Content Imaging Protocol for Measuring a Drug Induced DNA Damage Response Using Immunofluorescent Subnuclear γH2AX Spots in Patient Derived Ovarian Cancer Organoids

Hakan KELEŞ

Christopher A. Schofield

Helena Rannikmae Erin Elizabeth Edwards Lisa Mohamet

ACS Pharmacology & Translational Science Vol. 6 No. 1 pp. 12-21 · American Chemical Society (ACS)

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References

25

[1]

10.3390/ijms20040952

[2]

10.15252/embj.2019104013

[3]

10.1038/s41591-019-0422-6

[4]

10.1016/j.celrep.2020.107762

[5]

10.1186/s13048-018-0424-x

[6]

10.3390/cancers12051315

[7]

PARP inhibitors: Synthetic lethality in the clinic

Christopher J. Lord, Alan Ashworth

Science 10.1126/science.aam7344

[8]

10.1016/j.ajpath.2015.11.011

[9]

10.3389/fonc.2021.675972

[10]

10.1158/0008-5472.can-09-3788

[11]

10.1038/ncomms3126

[12]

10.18632/oncotarget.9929

[13]

10.3390/cancers13246288

[14]

10.18632/oncotarget.28220

[15]

10.1111/cas.13843

[16]

10.1126/science.aao2774

[17]

10.1038/s41598-022-09508-y

[18]

10.1177/1087057110392993

[19]

10.1038/s12276-021-00641-8

[20]

10.1016/j.chembiol.2014.07.015

[21]

10.1016/j.stem.2018.04.022

[22]

10.1371/journal.pone.0235319

[23]

10.1038/s41467-022-30722-9

[24]

10.1038/s41596-019-0160-8

[25]

10.1177/24725552211020668

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Patient-derived organoids in translational oncology and drug screening

Ruixin Yang, Yingyan Yu · 2023

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25

References

Details

Published: Dec 12, 2022
Vol/Issue: 6(1)
Pages: 12-21
License: View

Authors

H

Hakan KELEŞ

Genome Biology, Genomic Sciences, R&D, GSK, Gunnels Wood Road, Stevenage, SG1 2NY, United Kingdom

C

Christopher A. Schofield

Genome Biology, Genomic Sciences, R&D, GSK, Gunnels Wood Road, Stevenage, SG1 2NY, United Kingdom

H

Helena Rannikmae

Complex In Vitro Models, In Vitro In Vivo Translation, R&D, GSK, Gunnels Wood Road, Stevenage, SG1 2NY, United Kingdom

E

Erin Elizabeth Edwards

Genome Biology, Genomic Sciences, R&D, GSK, 1250 S. Collegeville Road, Collegeville, Pennsylvania 19426, United States

L

Lisa Mohamet

Genome Biology, Genomic Sciences, R&D, GSK, Gunnels Wood Road, Stevenage, SG1 2NY, United Kingdom

Cite This Article

Hakan KELEŞ, Christopher A. Schofield, Helena Rannikmae, et al. (2022). A Scalable 3D High-Content Imaging Protocol for Measuring a Drug Induced DNA Damage Response Using Immunofluorescent Subnuclear γH2AX Spots in Patient Derived Ovarian Cancer Organoids. ACS Pharmacology & Translational Science, 6(1), 12-21. https://doi.org/10.1021/acsptsci.2c00200

A Scalable 3D High-Content Imaging Protocol for Measuring a Drug Induced DNA Damage Response Using Immunofluorescent Subnuclear γH2AX Spots in Patient Derived Ovarian Cancer Organoids

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