PhosphoShield: Improving Trypsin Digestion of Phosphoproteins by Shielding the Negatively Charged Phosphate Moiety

Julia A. Bubis; Vladimir Gorshkov; Mikhail V. Gorshkov; Frank Kjeldsen

doi:10.1021/jasms.0c00171

journal article Aug 25, 2020

PhosphoShield: Improving Trypsin Digestion of Phosphoproteins by Shielding the Negatively Charged Phosphate Moiety

Julia A. Bubis

Vladimir Gorshkov

Mikhail V. Gorshkov

Frank Kjeldsen

Journal of the American Society for Mass Spectrometry Vol. 31 No. 10 pp. 2053-2060 · American Chemical Society (ACS)

View at Publisher Save 10.1021/jasms.0c00171

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References

45

[1]

10.1093/gigascience/giw015

[2]

10.1021/ac051896z

[3]

10.3892/ijmm.2017.3036

[4]

10.3389/fgene.2014.00270

[5]

Larsen M. R. (2016) 10.1016/b978-0-12-394447-4.40038-6

[6]

Structural Basis for Control by Phosphorylation

LOUISE N. JOHNSON, Richard J. Lewis

Chemical Reviews 10.1021/cr000225s

[7]

10.1074/mcp.t400003-mcp200

[8]

10.1074/jbc.m306944200

[9]

Binding of amino acid side-chains to S 1 cavities of serine proteinases 1 1Edited by R. Huber

Wuyuan Lu, Izydor Apostol, M.A. Qasim et al.

Journal of Molecular Biology 10.1006/jmbi.1996.0781

[10]

10.1110/ps.940101

[11]

10.1002/(sici)1097-0231(20000331)14:6<496::aid-rcm899>3.0.co;2-1

[12]

10.1073/pnas.0611217104

[13]

10.1016/j.celrep.2015.05.029

[14]

10.1039/c5mb00024f

[15]

10.1021/pr400802z

[16]

10.1021/pr401181y

[17]

10.1016/j.jprot.2015.05.009

[18]

10.1021/pr4008442

[19]

Value of Using Multiple Proteases for Large-Scale Mass Spectrometry-Based Proteomics

Danielle L. Swaney, Craig D. Wenger, Joshua J. Coon

Journal of Proteome Research 10.1021/pr900863u

[20]

10.1016/s0003-2670(99)00409-2

[21]

10.1111/febs.13287

[22]

10.1002/1096-9888(200012)35:12<1399::aid-jms86>3.0.co;2-r

[23]

10.1039/c5dt00729a

[24]

10.1021/acs.analchem.5b00257

[25]

10.1002/anie.201108481

[26]

10.1016/j.pep.2018.03.002

[27]

10.1074/mcp.tir118.000953

[28]

An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database

Jimmy K. Eng, Ashley L. McCormack, John R. Yates

Journal of the American Society for Mass Spectrome... 10.1016/1044-0305(94)80016-2

[29]

10.1007/s13361-015-1201-3

[30]

10.1021/pr200611n

[31]

10.1074/mcp.ra119.001777

[32]

limma powers differential expression analyses for RNA-sequencing and microarray studies

Matthew E. Ritchie, Belinda Phipson, Di Wu et al.

Nucleic Acids Research 10.1093/nar/gkv007

[33]

10.2202/1544-6115.1027

[34]

A Direct Approach to False Discovery Rates

John D. Storey

Journal of the Royal Statistical Society Series B:... 10.1111/1467-9868.00346

[35]

The PRIDE database and related tools and resources in 2019: improving support for quantification data

Yasset Perez-Riverol, Attila Csordas, Jingwen Bai et al.

Nucleic Acids Research 10.1093/nar/gky1106

[36]

Harman J. C. Mol. Vis. (2018)

[37]

10.1016/j.celrep.2014.07.036

[38]

10.1096/fj.201900445r

[39]

10.1021/bi0508690

[40]

10.1107/s0907444905033627

[41]

10.1016/j.csbj.2019.06.022

[42]

Salt bridges: Geometrically specific, designable interactions

Jason E. Donald, Daniel W. Kulp, William F. DeGrado

Proteins: Structure, Function, and Bioinformatics 10.1002/prot.22927

[43]

10.1002/anie.201508601

[44]

Grad J.-N. Evaluation (2005)

[45]

Equilibrium constants for association of guanidinium and ammonium ions with oxyanions

Bleecker Springs, Paul Haake

Bioorganic Chemistry 10.1016/0045-2068(77)90019-0

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References

Details

Published: Aug 25, 2020
Vol/Issue: 31(10)
Pages: 2053-2060
License: View

Authors

J

Julia A. Bubis

V. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia; Moscow Institute of Physics and Technology (State University), 141700 Dolgoprudny, Russia; Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark

V

Vladimir Gorshkov

Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark

M

Mikhail V. Gorshkov

V. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia

F

Frank Kjeldsen

Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark

Funding

H2020 European Research Council Award: 646603

Russian Academy of Sciences Award: 0068-2019-0011

Ministry of Education and Science of the Russian Federation

Cite This Article

Julia A. Bubis, Vladimir Gorshkov, Mikhail V. Gorshkov, et al. (2020). PhosphoShield: Improving Trypsin Digestion of Phosphoproteins by Shielding the Negatively Charged Phosphate Moiety. Journal of the American Society for Mass Spectrometry, 31(10), 2053-2060. https://doi.org/10.1021/jasms.0c00171

PhosphoShield: Improving Trypsin Digestion of Phosphoproteins by Shielding the Negatively Charged Phosphate Moiety

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