Functional rafts in cell membranes

Kai Simons; Elina Ikonen

doi:10.1038/42408

journal article Jun 01, 1997

Functional rafts in cell membranes

Kai Simons

Elina Ikonen

Nature Vol. 387 No. 6633 pp. 569-572 · Springer Science and Business Media LLC

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References

67

[1]

The Fluid Mosaic Model of the Structure of Cell Membranes

S. J. Singer, Garth L. Nicolson

Science 1972 10.1126/science.175.4023.720

[2]

van Meer, G. Lipid traffic in animal cells. Annu. Rev. Cell Biol. 5, 247–275 (1989). 10.1146/annurev.cb.05.110189.001335

[3]

Kusumi, A. & Sako, Y. Cell surface organization by the membrane skeleton. Curr. Opin. Cell Biol. 8, 566–574 (1996). 10.1016/s0955-0674(96)80036-6

[4]

Rodriguez-Boulan, E. & Nelson, W. J. Morphogenesis of the polarized epithelial cell phenotype. Science 245, 718–725 (1989). 10.1126/science.2672330

[5]

Simons, K. & van Meer, G. Lipid sorting in epithelial cells. Biochemistry 27, 6197–6202 (1988). 10.1021/bi00417a001

[6]

van Helvoort, A. et al. MDR1 P-glycoprotein is a lipid translocase of broad specificity, while M P-glycoprotein specifically translocates phosphatidylcholine. Cell 87, 507–518 (1996). 10.1016/s0092-8674(00)81370-7

[7]

Brown, D. & Rose, J. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68, 533–544 (1992). 10.1016/0092-8674(92)90189-j

[8]

Parton, R. G. Caveolae and caveolins. Curr. Opin. Cell Biol. 8, 542–548 (1996). 10.1016/s0955-0674(96)80033-0

[9]

Tran, D. J., Carpentier, J. L., Sawano, F., Gorden, P. & Orci, L. Ligands internalized through coated or non-coated invaginations follow a common intracellular pathway. Proc. Natl Acad. Sci. USA 84, 7957–7961 (1987). 10.1073/pnas.84.22.7957

[10]

Rothberg, K. G., Ying, Y. S., Kamen, B. A. & Anderson, R. G. W. Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate. J. Cell Biol. 111, 2931–2938 (1990). 10.1083/jcb.111.6.2931

[11]

Ghitescu, L., Fixman, A., Simionescu, M. & Simionescu, N. Specific binding sites for albumin restricted to plasmalemmal vesicles of continuous capillary endothelium: receptor-mediated transcytosis. J. Cell Biol. 102, 1304–1311 (1986). 10.1083/jcb.102.4.1304

[12]

Dupree, P., Parton, R. G., Raposo, G., Kurzchalia, T. V. & Simons, K. Caveolae and sorting in the trans-Golgi network of epithelial cells. EMBO J. 12, 1597–1605 (1993). 10.1002/j.1460-2075.1993.tb05804.x

[13]

Morrow, M. R., Singh, D., Lu, D. & Grant, C. W. M. Glycosphingolipid fatty acid arrangement in phospholipid bilayers: cholesterol effects. Biophysical J. 68, 179–186 (1995). 10.1016/s0006-3495(95)80173-6

[14]

Boggs, J. M. & Koshy, K. M. Do the long fatty acid chains of sphingolipids interdigitate across the center of bilayer of shorter chain symmetric phospholipids? Biochim. Biophys. Acta 1189, 233–241 (1994). 10.1016/0005-2736(94)90070-1

[15]

Parton, R. G. & Simons, K. Digging into caveolae. Science 269, 1398–1399 (1995). 10.1126/science.7660120

[16]

Schroeder, R., London, E. & Brown, D. Interactions between saturated acyl chains confer detergent resistance on lipids and glycosylphosphatidylinositol(GPI)-anchored proteins: GPI-anchored proteins in liposomes and cells show similar behavior. Proc. Natl Acad. Sci. USA 91, 12130–12134 (1994). 10.1073/pnas.91.25.12130

[17]

Skibbens, J. E., Roth, M. G. & Matlin, K. S. Differential extractibility of influenza virus hemagglutinin during intracellular transport in polarized epithelial cells and nonpolar fibroblasts. J. Cell Biol. 108, 821–832 (1989). 10.1083/jcb.108.3.821

[18]

Sargiacomo, M., Sudol, M., Tang, Z. & Lisanti, M. P. Signal transducing molecules and GPI-linked proteins from a caveolin-rich insoluble complex in MDCK cells. J. Cell Biol. 122, 789–807 (1993). 10.1083/jcb.122.4.789

[19]

Fra, A. M., Williamson, E., Simons, K. & Parton, R. G. Detergent-insoluble glycolipid microdomains in lymphocytes in the absence of caveolae. J. Biol. Chem. 269, 30745–30748 (1994). 10.1016/s0021-9258(18)47340-1

[20]

Danielsen, E. M. Atransferrin-like GPI-linked iron-binding protein in detergent-insoluble noncaveolar microdomains at the apical surface of fetal intestinal epithelial cells. Biochemistry 34, 1596–1605 (1995). 10.1021/bi00005a016

[21]

Casey, P. J. Protein lipidation in cell signalling. Science 268, 221–225 (1995). 10.1126/science.7716512

[22]

Cerneus, D. P., Ueffing, E., Posthuma, G., Strous, G. J. & van der Ende, A. Detergent insolubility of alkaline phosphatase during biosynthetic transport and endocytosis. Role of cholesterol. J. Biol. Chem. 268, 3150–3155 (1993). 10.1016/s0021-9258(18)53671-1

[23]

Hanada, K., Nishijima, M., Akamatsu, Y. & Pagano, R. E. Both sphingolipids and cholesterol participate in the detergent-insolubility of alkaline phosphatase, a glycosyl-phosphatidylinositol anchored protein in mammalian cells. J. Biol. Chem. 270, 6254–6260 (1995). 10.1074/jbc.270.11.6254

[24]

Hannan, L. A. & Edidin, M. Glycosylphosphatidylinositol-anchored protein after LDL-deprivation of MDCK cells. J. Cell Biol. 133, 1265–1276 (1996). 10.1083/jcb.133.6.1265

[25]

Kundu, A., Avalos, R. T., Sanderson, C. M. & Nayak, D. P. Transmembrane domain of influenza virus neuraminidase, a type II protein, possesses an apical sorting signal in polarized MDCK cells. J. Virol. 70, 6508–6515 (1996). 10.1128/jvi.70.9.6508-6515.1996

[26]

Murata, M. et al. VIP21-caveolin is a cholesterol-binding protein. Proc. Natl Acad. Sci. USA 92, 10339–10343 (1995). 10.1073/pnas.92.22.10339

[27]

Lisanti, M. P., Sargiacomo, M., Graeve, L., Saltiel, A. & Rodriguez-Boulan, E. Polarized apical distribution of glycosyl-phosphatidylinositol-anchored proteins in a renal epithelial cell line. Proc. Natl Acad. Sci. USA 85, 9557–9561 (1989). 10.1073/pnas.85.24.9557

[28]

Scheiffele, P., Peränen, J. & Simons, K. N-glycans as apical sorting signals in epithelial cells. Nature 378, 96–98 (1995). 10.1038/378096a0

[29]

Fiedler, K., Parton, R. G., Kellner, R., Etzold, T. & Simons, K. VIP36, a novel component of glycolipid rafts and exocytic carrier vesicles in epithelial cells. EMBO J. 13, 1729–1740 (1994). 10.1002/j.1460-2075.1994.tb06437.x

[30]

Matter, K. & Mellman, I. Mechanisms of cell polarity: Sorting and transport in epithelial cells. Curr. Opin. Cell. Biol. 6, 545–554 (1994). 10.1016/0955-0674(94)90075-2

[31]

Ikonen, E., Tagaya, M., Ullrich, O., Montecucco, C. & Simons, K. Different requirements for NSF, SNAP and Rab proteins in apical and basolateral transport in MDCK cells. Cell 81, 1–20 (1995). 10.1016/0092-8674(95)90078-0

[32]

Rothman, J. E. & Wieland, F. T. Protein sorting by transport vesicles. Science 272, 227–234 (1996). 10.1126/science.272.5259.227

[33]

Müsch, A., Xu, H., Shield, D. & Rodriguez-Boulan, E. Transport of vesicular stomatitis virus G protein to the cell surface is signal mediated in polarized and nonpolarized cells. J. Cell Biol. 133, 543–558 (1996). 10.1083/jcb.133.3.543

[34]

Yoshimori, T., Keller, P., Roth, M. G. & Simons, K. Different biosynthetic transport routes to the plasma membrane in BHK and CHO cells. J. Cell Biol. 133, 247–256 (1996). 10.1083/jcb.133.2.247

[35]

Danielsen, E. M. & van Deurs, B. Involvement of detergent-insoluble complexes in the intracellular transport of intestinal brush border enzymes. J. Cell Biol. 131, 939–950 (1995). 10.1083/jcb.131.4.939

[36]

Schnitzer, J. E., Oh, P., Pinney, E. & Allard, J. Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis and capillary permeability of select macromolecules. J. Cell Biol. 127, 1217–1232 (1994). 10.1083/jcb.127.5.1217

[37]

Anderson, R. G. W., Kamen, B. A., Rothberg, K. G. & Lacey, S. W. Potocytosis: sequestration and transport of small molecules by caveolae. Science 255, 410–411 (1992). 10.1126/science.1310359

[38]

Anderson, H. A., Chen, Y. & Norkin, L. C. Bound simian virus 40 translocates to caveolin-enriched membrane domains and its entry is inhibited by drugs that selectively disrupt caveolae. Mol. Biol. Cell 7, 1825–1834 (1996). 10.1091/mbc.7.11.1825

[39]

Deckert, M. et al. Endocytosis of GPI-anchored proteins in human lymphocytes: role of glycolipid-based domains, actin cytoskeleton and protein kinases. J. Cell Biol. 133, 791–799 (1996). 10.1083/jcb.133.4.791

[40]

Mostov, K. E., Apodaca, G., Aroeti, B. & Okamoto, C. Plasma membrane protein sorting in polarized epithelial cells. J. Cell Biol. 116, 577–583 (1992). 10.1083/jcb.116.3.577

[41]

Simons, K. et al. The biogenesis of cell surface polarity in epithelial cells and neurons. Cold Spring Harbor Symp. Quant. Biol. LVII, 611–619 (1992). 10.1101/sqb.1992.057.01.067

[42]

Pfeiffer, S. E., Warrington, A. E. & Bansal, R. The oligodendrocyte and its many cellular processes. Trends Cell Biol. 3, 191–197 (1993). 10.1016/0962-8924(93)90213-k

[43]

Peränen, J., Auvinen, P., Virta, H., Wepf, R. & Simons, K. Rab8 promotes polarized membrane transport through reorganization of actin and microtubules in fibroblasts. J. Cell Biol. 135, 153–167 (1996). 10.1083/jcb.135.1.153

[44]

Anderson, R. G. W. Caveolae: where incoming and outgoing messengers meet. Proc. Natl Acad. Sci. USA 90, 10909–10913 (1993). 10.1073/pnas.90.23.10909

[45]

Lisanti, M. P., Scherer, P. E., Tang, Z. L. & Sargiacomo, M. Caveolaer, caveolin and caveolin-rich membrane domains: a signalling hypothesis. Trends Cell Biol. 4, 231–235 (1994). 10.1016/0962-8924(94)90114-7

[46]

Parpal, S., Gustavsson, J. & Strålfors, P. Isolation of phosphooligosaccharide/phosphoinositol glycan from caveolae and cytosol of insulin-stimulated cells. J. Cell Biol. 131, 125–135 (1995). 10.1083/jcb.131.1.125

[47]

Li, S. et al. Evidence for a regulated interaction between heterotrimeric G proteins and caveolin. J. Biol. Chem. 270, 15693–15701 (1995). 10.1074/jbc.270.26.15693

[48]

Song, K. S. et al. Co-purification and direct interaciton of Ras with caveolin, an integral membrane protein of caveolae microdomains. J. Biol. Chem. 271, 9690–9697 (1996). 10.1074/jbc.271.16.9690

[49]

Mineo, C., James, G. L., Smart, E. J. & Anderson, R. G. Localization of epidermal growth factor-stimulated Ras/Raf-1 interaction to caveolae membrane. J. Biol. Chem. 217, 11930–11935 (1996). 10.1074/jbc.271.20.11930

[50]

Hope, H. R. & Pike, L. J. Phosphoinositides and phosphoinositide-utilizing enzymes in detergent-insoluble lipid domains. Mol. Biol. Cell 7, 843–851 (1996). 10.1091/mbc.7.6.843

Showing 50 of 67 references

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