journal article Jul 01, 2000

TnAraOut, A transposon-based approach to identify and characterize essential bacterial genes

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References
35
[1]
Clayton, R.A., White, O. & Fraser, C.M. Findings emerging from complete microbial genome sequences. Curr. Opin. Microbiol. 1, 562–566 (1998). 10.1016/s1369-5274(98)80089-1
[2]
Tang, C.M., Hood, D.W. & Moxon, E.R. Microbial genome sequencing and pathogenesis. Curr. Opin. Microbiol. 1, 12–16 (1998). 10.1016/s1369-5274(98)80137-9
[3]
Schmid, M.B., Kapur, N., Isaacson, D.R., Lindroos, P. & Sharpe, C. Genetic analysis of temperature-sensitive lethal mutants of Salmonella typhimurium. Genetics 123, 625–633 (1989). 10.1093/genetics/123.4.625
[4]
Arigoni, F. et al. A genome-based approach for the identification of essential bacterial genes. Nat. Biotechnol. 16, 851–856 (1998). 10.1038/nbt0998-851
[5]
Mushegian, A.R. & Koonin, E.V. A minimal gene set for cellular life derived by comparison of complete bacterial genomes [see comments]. Proc. Natl. Acad. Sci. USA 93, 10268–10273 (1996). 10.1073/pnas.93.19.10268
[6]
Akerley, B.J. et al. Systematic identification of essential genes by in vitro mariner mutagenesis. Proc. Natl. Acad. Sci. USA 95, 8927–8932 (1998). 10.1073/pnas.95.15.8927
[7]
Hutchison, C.A. et al. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286, 2165–2169 (1999). 10.1126/science.286.5447.2165
[8]
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

L M Guzman, D Belin, M J Carson et al.

Journal of Bacteriology 1995 10.1128/jb.177.14.4121-4130.1995
[9]
Rubin, E.J. et al. In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria. Proc. Natl. Acad. Sci. USA 96, 1645–1650 (1999). 10.1073/pnas.96.4.1645
[10]
Gueiros-Filho, F.J. & Beverley, S.M. Trans-kingdom transposition of the Drosophila element mariner within the protozoan Leishmania (see comments). Science 276, 1716–1719 (1997). (Published erratum appears in Science 277, 753, 1997.) 10.1126/science.276.5319.1716
[11]
Harley, C.B. & Reynolds, R.P. Analysis of E. coli promoter sequences. Nucleic Acids Res. 15, 2343–2361 (1987). 10.1093/nar/15.5.2343
[12]
Takiff, H.E., Baker, T., Copeland, T., Chen, S.M. & Court, D.L. Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon. J. Bacteriol. 174, 1544–1553 (1992). 10.1128/jb.174.5.1544-1553.1992
[13]
Rappleye, C.A. & Roth, J.R. A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes. J. Bacteriol. 179, 5827–5834 (1997). 10.1128/jb.179.18.5827-5834.1997
[14]
Chow, W.Y. & Berg, D.E. Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations. Proc. Natl. Acad. Sci.USA 85, 6468–6472 (1988). 10.1073/pnas.85.17.6468
[15]
Fullner, K.J. & Mekalanos, J.J. Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. Infect. Immun. 67, 1393–1404 (1999). 10.1128/iai.67.3.1393-1404.1999
[16]
Preliminary sequence data was obtained from The Institute for Genomic Research website at http://www.tigr.org .
[17]
Heidelberg, J.F. et al. Whole genome sequencing of Vibrio cholerae, the etiologic agent of cholera. In Proceedings of the 35th US–Japan Cholera and Other Bacterial Enteric Infections Joint Panel Meeting 87 (Baltimore, MD; 1999).
[18]
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

S. Altschul

Nucleic Acids Research 1997 10.1093/nar/25.17.3389
[19]
Schimmel, P., 19. Tao, J. & Hill, J. Aminoacyl tRNA synthetases as targets for new anti-infectives. FASEB J. 12, 1599–1609 (1998). 10.1096/fasebj.12.15.1599
[20]
Samuels, D.S., Marconi, R.T., Huang, W.M. & Garon, C.F. gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi. J. Bacteriol. 176, 3072–3075 (1994). 10.1128/jb.176.10.3072-3075.1994
[21]
Hirokawa, T., Boon-Chieng, S. & Mitaku, S. SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14, 378–379 (1998). 10.1093/bioinformatics/14.4.378
[22]
Expert system for predicting protein localization sites in gram‐negative bacteria

Kenta Nakai, Minoru Kanehisa

Proteins: Structure, Function, and Bioinformatics 1991 10.1002/prot.340110203
[23]
Trucksis, M., Michalski, J., Deng, Y.K. & Kaper, J.B. The Vibrio cholerae genome contains two unique circular chromosomes. Proc. Natl. Acad. Sci.USA 95, 14464–14469 (1998). 10.1073/pnas.95.24.14464
[24]
Curnow, A.W., Tumbula, D.L., Pelaschier, J.T., Min, B. & Soll, D. Glutamyl-tRNA(Gln) amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis. Proc. Natl. Acad. Sci.USA 95, 12838–12843 (1998). 10.1073/pnas.95.22.12838
[25]
Ibba, M., Curnow, A.W. & Soll, D. Aminoacyl-tRNA synthesis: divergent routes to a common goal. Trends Biochem. Sci. 22, 39–42 (1997). 10.1016/s0968-0004(96)20033-7
[26]
Irani, M.H. & Maitra, P.K. Properties of Escherichia coli mutants deficient in enzymes of glycolysis. J. Bacteriol. 132, 398–410 (1977). 10.1128/jb.132.2.398-410.1977
[27]
Chiang, S.L. & Mekalanos, J.J. Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol. Microbiol. 27, 797–805 (1998). 10.1046/j.1365-2958.1998.00726.x
[28]
Schleif, R. Two positively regulated systems, ara and mal. In Escherichia coli and Salmonella, Vol. 1 (ed. Neidhardt, F.C.) 1300–1309 (ASM Press, Washington, DC; 1996).
[29]
Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular cloning: a laboratory manual. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; 1989).
[30]
Conditionally Replicative and Conjugative Plasmids CarryinglacZα for Cloning, Mutagenesis, and Allele Replacement in Bacteria

William W. Metcalf, Weihong Jiang, Larry L. Daniels et al.

Plasmid 1996 10.1006/plas.1996.0001
[31]
Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K

Roberto Kolter, Manabu Inuzuka, Donald R. Helinski

Cell 1978 10.1016/0092-8674(78)90046-6
[32]
Guiney, D.G. & Helinski, D.R. The DNA–protein relaxation complex of the plasmid RK2: location of the site-specific nick in the region of the proposed origin of transfer. Mol. Gen. Genet. 176, 183–189 (1979). 10.1007/bf00273212
[33]
Mekalanos, J.J. Duplication and amplification of toxin genes in Vibrio cholerae. Cell 35, 253–263 (1983). 10.1016/0092-8674(83)90228-3
[35]
Miller, J.H. Experiments in molecular genetics. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY; 1972).
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Published
Jul 01, 2000
Vol/Issue
18(7)
Pages
740-745
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Cite This Article
Nicholas Judson, John J. Mekalanos (2000). TnAraOut, A transposon-based approach to identify and characterize essential bacterial genes. Nature Biotechnology, 18(7), 740-745. https://doi.org/10.1038/77305