Optogenetic control of nuclear protein export

Dominik Niopek; Pierre Wehler; Julia Roensch; Roland Eils; Barbara Di Ventura

doi:10.1038/ncomms10624

journal article Open Access Feb 08, 2016

Optogenetic control of nuclear protein export

Dominik Niopek

Pierre Wehler Julia Roensch Roland Eils

Barbara Di Ventura

Nature Communications Vol. 7 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/ncomms10624

Abstract

AbstractActive nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export system (LEXY) based on a single, genetically encoded tag, which enables precise spatiotemporal control over the export of tagged proteins. A constitutively nuclear, chromatin-anchored LEXY variant expands the method towards light inhibition of endogenous protein export by sequestering cellular CRM1 receptors. We showcase the utility of LEXY for cell biology applications by regulating a synthetic repressor as well as human p53 transcriptional activity with light. LEXY is a powerful addition to the optogenetic toolbox, allowing various novel applications in synthetic and cell biology.

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Published: Feb 08, 2016
Vol/Issue: 7(1)
License: View

Authors

Mammalian Developmental Epigenetics Group Institut Curie CNRS UMR3215, INSERM U934 PSL University Paris France

R

Roland Eils

1 Division of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), 2 Heidelberg Center for Personalized Oncology (DKFZ-HIPO) and 3 Department for Bioinformatics and Functional Genomics, Institute for Pharmacy and Molecular Biotechnology (IPMB) and BioQuant Center, Heidelberg University, 69120 Heidelberg, Germany; 1 Division of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), 2 Heidelberg Center for Personalized Oncology (DKFZ-HIPO) and 3 Department for Bioinformatics and Functional Genomics, Institute for Pharmacy and Molecular Biotechnology (IPMB) and BioQuant Center, Heidelberg University, 69120 Heidelberg, Germany; 1 Division of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), 2 Heidelberg Center for Personalized Oncology (DKFZ-HIPO) and 3 Department for Bioinformatics and Functional Genomics, Institute for Pharmacy and Molecular Biotechnology (IPMB) and BioQuant Center, Heidelberg University, 69120 Heidelberg, Germany

B

Barbara Di Ventura

Cite This Article

Dominik Niopek, Pierre Wehler, Julia Roensch, et al. (2016). Optogenetic control of nuclear protein export. Nature Communications, 7(1). https://doi.org/10.1038/ncomms10624

Optogenetic control of nuclear protein export

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