Loss of lamin A function increases chromatin dynamics in the nuclear interior

I. Bronshtein; E. Kepten; I. Kanter; S. Berezin; M. Lindner; Abena B. Redwood; S Mai; S. Gonzalo; R. Foisner; Y. Shav-Tal; Y. Garini

doi:10.1038/ncomms9044

journal article Open Access Aug 24, 2015

Loss of lamin A function increases chromatin dynamics in the nuclear interior

I. Bronshtein E. Kepten I. Kanter S. Berezin M. Lindner

Abena B. Redwood S Mai S. Gonzalo

R. Foisner Y. Shav-Tal Y. Garini

Nature Communications Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/ncomms9044

Abstract

AbstractChromatin is organized in a highly ordered yet dynamic manner in the cell nucleus, but the principles governing this organization remain unclear. Similarly, it is unknown whether, and how, various proteins regulate chromatin motion and as a result influence nuclear organization. Here by studying the dynamics of different genomic regions in the nucleus of live cells, we show that the genome has highly constrained dynamics. Interestingly, depletion of lamin A strikingly alters genome dynamics, inducing a dramatic transition from slow anomalous diffusion to fast and normal diffusion. In contrast, depletion of LAP2α, a protein that interacts with lamin A and chromatin, has no such effect on genome dynamics. We speculate that chromosomal inter-chain interactions formed by lamin A throughout the nucleus contribute to chromatin dynamics, and suggest that the molecular regulation of chromatin diffusion by lamin A in the nuclear interior is critical for the maintenance of genome organization.

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Published: Aug 24, 2015
Vol/Issue: 6(1)
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Cite This Article

I. Bronshtein, E. Kepten, I. Kanter, et al. (2015). Loss of lamin A function increases chromatin dynamics in the nuclear interior. Nature Communications, 6(1). https://doi.org/10.1038/ncomms9044

Loss of lamin A function increases chromatin dynamics in the nuclear interior

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