STED super-resolved microscopy

Giuseppe Vicidomini; Paolo Bianchini; Alberto Diaspro

doi:10.1038/nmeth.4593

journal article Jan 29, 2018

STED super-resolved microscopy

Giuseppe Vicidomini

Paolo Bianchini

Alberto Diaspro

Nature Methods Vol. 15 No. 3 pp. 173-182 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/nmeth.4593

Topics

No keywords indexed for this article. Browse by subject →

References

83

[1]

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy

Stefan W. Hell, Jan Wichmann

Optics Letters 1994 10.1364/ol.19.000780

[2]

Hell, S.W. Microscopy and its focal switch. Nat. Methods 6, 24–32 (2009). 10.1038/nmeth.1291

[3]

Diaspro, A. ed. Nanoscopy and Multidimensional Optical Fluorescence Microscopy (Chapman and Hall/CRC, 2010). 10.1201/9781420078893

[4]

Eggeling, C., Willig, K.I., Sahl, S.J. & Hell, S.W. Lens-based fluorescence nanoscopy. Q. Rev. Biophys. 48, 178–243 (2015). 10.1017/s0033583514000146

[5]

Hell, S.W. Far-field optical nanoscopy. Science 316, 1153–1158 (2007). 10.1126/science.1137395

[6]

Klein, T., Proppert, S. & Sauer, M. Eight years of single-molecule localization microscopy. Histochem. Cell Biol. 141, 561–575 (2014). 10.1007/s00418-014-1184-3

[7]

Bianchini, P. et al. STED nanoscopy: a glimpse into the future. Cell Tissue Res. 360, 143–150 (2015). 10.1007/s00441-015-2146-3

[8]

Born, M. & Wolf, E. Principles of Optics (Cambridge University Press, 1999).

[9]

Tortarolo, G., Castello, M., Diaspro, A., Koho, S. & Vicidomini, G. Evaluating image resolution in STED microscopy. Optica 5, 32–35 (2018). 10.1364/optica.5.000032

[10]

Galiani, S. et al. Strategies to maximize the performance of a STED microscope. Opt. Express 20, 7362–7374 (2012). 10.1364/oe.20.007362

[11]

Vicidomini, G. et al. STED nanoscopy with time-gated detection: theoretical and experimental aspects. PLoS One 8, e54421 (2013). 10.1371/journal.pone.0054421

[12]

Hotta, J. et al. Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores. J. Am. Chem. Soc. 132, 5021–5023 (2010). 10.1021/ja100079w

[13]

Wildanger, D., Bückers, J., Westphal, V., Hell, S.W. & Kastrup, L. A STED microscope aligned by design. Opt. Express 17, 16100–16110 (2009). 10.1364/oe.17.016100

[14]

Reuss, M., Engelhardt, J. & Hell, S.W. Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation. Opt. Express 18, 1049–1058 (2010). 10.1364/oe.18.001049

[15]

Yan, L. et al. Q-plate enabled spectrally diverse orbital-angular-momentum conversion for stimulated emission depletion microscopy. Optica 2, 900–903 (2015). 10.1364/optica.2.000900

[16]

Gould, T.J., Kromann, E.B., Burke, D., Booth, M.J. & Bewersdorf, J. Auto-aligning stimulated emission depletion microscope using adaptive optics. Opt. Lett. 38, 1860–1862 (2013). 10.1364/ol.38.001860

[17]

Klar, T.A., Jakobs, S., Dyba, M., Egner, A. & Hell, S.W. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc. Natl. Acad. Sci. USA 97, 8206–8210 (2000). 10.1073/pnas.97.15.8206

[18]

Nanoscale resolution in GFP-based microscopy

Katrin I Willig, Robert R Kellner, Rebecca Medda et al.

Nature Methods 2006 10.1038/nmeth922

[19]

Westphal, V., Blanca, C.M., Dyba, M., Kastrup, L. & Hell, S.W. Laser-diode-stimulated emission depletion microscopy. Appl. Phys. Lett. 82, 3125 (2003). 10.1063/1.1571656

[20]

Rankin, B.R., Kellner, R.R. & Hell, S.W. Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source. Opt. Lett. 33, 2491–2493 (2008). 10.1364/ol.33.002491

[21]

Göttfert, F. et al. Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution. Biophys. J. 105, L01–L03 (2013). 10.1016/j.bpj.2013.05.029

[22]

Bottanelli, F. et al. Two-colour live-cell nanoscale imaging of intracellular targets. Nat. Commun. 7, 10778 (2016). 10.1038/ncomms10778

[23]

Vicidomini, G. et al. Sharper low-power STED nanoscopy by time gating. Nat. Methods 8, 571–573 (2011). 10.1038/nmeth.1624

[24]

Wildanger, D., Rittweger, E., Kastrup, L. & Hell, S.W. STED microscopy with a supercontinuum laser source. Opt. Express 16, 9614–9621 (2008). 10.1364/oe.16.009614

[25]

Bückers, J., Wildanger, D., Vicidomini, G., Kastrup, L. & Hell, S.W. Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses. Opt. Express 19, 3130–3143 (2011). 10.1364/oe.19.003130

[26]

Winter, F.R. et al. Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection. Sci. Rep. 7, 46492 (2017).

[27]

Bianchini, P., Harke, B., Galiani, S., Vicidomini, G. & Diaspro, A. Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging. Proc. Natl. Acad. Sci. USA 109, 6390–6393 (2012). 10.1073/pnas.1119129109

[28]

van der Velde, J.H.M. et al. A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization. Nat. Commun. 7, 10144 (2016). 10.1038/ncomms10144

[29]

Donnert, G. et al. Macromolecular-scale resolution in biological fluorescence microscopy. Proc. Natl. Acad. Sci. USA 103, 11440–11445 (2006). 10.1073/pnas.0604965103

[30]

Moneron, G. et al. Fast STED microscopy with continuous wave fiber lasers. Opt. Express 18, 1302–1309 (2010). 10.1364/oe.18.001302

[31]

Schneider, J. et al. Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics. Nat. Methods 12, 827–830 (2015). 10.1038/nmeth.3481

[32]

Beater, S., Holzmeister, P., Pibiri, E., Lalkens, B. & Tinnefeld, P. Choosing dyes for cw-STED nanoscopy using self-assembled nanorulers. Phys. Chem. Chem. Phys. 16, 6990–6996 (2014). 10.1039/c4cp00127c

[33]

Han, K.Y. et al. Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light. Nano Lett. 9, 3323–3329 (2009). 10.1021/nl901597v

[34]

Lesoine, M.D. et al. Subdiffraction, luminescence-depletion imaging of isolated, giant, CdSe/CdS nanocrystal quantum dots. J. Phys. Chem. C 117, 3662–3667 (2013). 10.1021/jp312231k

[35]

Liu, Y. et al. Amplified stimulated emission in upconversion nanoparticles for super-resolution nanoscopy. Nature 543, 229–233 (2017). 10.1038/nature21366

[36]

Staudt, T. et al. Far-field optical nanoscopy with reduced number of state transition cycles. Opt. Express 19, 5644–5657 (2011). 10.1364/oe.19.005644

[37]

Göttfert, F. et al. Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proc. Natl. Acad. Sci. USA 114, 2125–2130 (2017). 10.1073/pnas.1621495114

[38]

Heine, J. et al. Adaptive-illumination STED nanoscopy. Proc. Natl. Acad. Sci. USA 114, 9797–9802 (2017). 10.1073/pnas.1708304114

[39]

Danzl, J.G. et al. Coordinate-targeted fluorescence nanoscopy with multiple off states. Nat. Photonics 10, 122–128 (2016). 10.1038/nphoton.2015.266

[40]

Castello, M. et al. Gated-sted microscopy with subnanosecond pulsed fiber laser for reducing photobleaching. Microsc. Res. Tech. 79, 785–791 (2016).

[41]

Strack, R. Death by super-resolution imaging. Nat. Methods 12, 1111 (2015). 10.1038/nmeth.3676

[42]

Light-induced cell damage in live-cell super-resolution microscopy

Sina Wäldchen, Julian Lehmann, Teresa Klein et al.

Scientific Reports 2015 10.1038/srep15348

[43]

Laissue, P.P., Alghamdi, R.A., Tomancak, P., Reynaud, E.G. & Shroff, H. Assessing phototoxicity in live fluorescence imaging. Nat. Methods 14, 657–661 (2017). 10.1038/nmeth.4344

[44]

Hein, B., Willig, K.I. & Hell, S.W. Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell. Proc. Natl. Acad. Sci. USA 105, 14271–14276 (2008). 10.1073/pnas.0807705105

[45]

Schermelleh, L., Heintzmann, R. & Leonhardt, H. A guide to super-resolution fluorescence microscopy. J. Cell Biol. 190, 165–175 (2010). 10.1083/jcb.201002018

[46]

Lukinavičius, G. et al. Fluorogenic probes for live-cell imaging of the cytoskeleton. Nat. Methods 11, 731–733 (2014). 10.1038/nmeth.2972

[47]

Lukinavičius, G. et al. SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy. Nat. Commun. 6, 8497 (2015). 10.1038/ncomms9497

[48]

Butkevich, A.N. et al. Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells. Angew. Chem. Int. Ed. Engl. 55, 3290–3294 (2016). 10.1002/anie.201511018

[49]

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

Gražvydas Lukinavičius, Keitaro Umezawa, Nicolas Olivier et al.

Nature Chemistry 2013 10.1038/nchem.1546

[50]

Matela, G. et al. A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy. Chem. Commun. (Camb.) 53, 979–982 (2017). 10.1039/c6cc09081h

Showing 50 of 83 references

Cited By

665

Recent Advances in Single‐Molecule Localization Based Super‐Resolution Imaging

Yen Leng Pak, Yingying Jing · 2026

Small

Localization of nanoscale objects with light singularities

Thomas A. Grant, Anton N. Vetlugin · 2025

Nanophotonics

Localizing axial dense emitters based on single‐helix point spread function and compressed sensing

Hanzhe Wu, Danni Chen · 2025

Nanophotonics

Fluorescent labeling strategies for molecular bioimaging

Marcel Streit, Made Budiarta · 2025

Biophysical Reports

Modern Methods of Fluorescence Nanoscopy in Biology (A Review)

D. О. Solovyevа, А. V. Altuninа · 2024

Russian Journal of Bioorganic Chemi...

Renal macrophages monitor and remove particles from urine to prevent tubule obstruction

Jian He, Yangyang Cao · 2024

Immunity

Binary amplitude holograms for shaping complex light fields with digital micromirror devices

R Gutiérrez-Cuevas, S M Popoff · 2024

Journal of Physics: Photonics

IgGFc-binding protein and MUC2 mucin produced by colonic goblet-like cells spatially interact non-covalently and regulate wound healing

Hayley Gorman, France Moreau · 2023

Frontiers in Immunology

Dissipative Soliton Mode-Locked Erbium-Doped Fiber Laser Using Nb2AlC Nanomaterial Saturable Absorber

Arni Munira Markom, Nurul Athirah Mohamad Abdul Ghafar · 2023

Nanomaterials

Writing and reading with the longitudinal component of light using carbazole-containing azopolymer thin films

Alexey Porfirev, Svetlana Khonina · 2022

Scientific Reports

Physical limits in electromagnetism

Pengning Chao, Benjamin Strekha · 2022

Nature Reviews Physics

Nanoscale Focus Pinspot for High-Purity Quantum Emitters via Focused-Ion-Beam-Induced Luminescence Quenching

Minho Choi, Seongmoon Jun · 2021

ACS Nano

Metrics

665

Citations

83

References

Details

Published: Jan 29, 2018
Vol/Issue: 15(3)
Pages: 173-182
License: View

Authors

Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy

A

Alberto Diaspro

Cite This Article

Giuseppe Vicidomini, Paolo Bianchini, Alberto Diaspro (2018). STED super-resolved microscopy. Nature Methods, 15(3), 173-182. https://doi.org/10.1038/nmeth.4593

STED super-resolved microscopy

You May Also Like