Protein complex expression by using multigene baculoviral vectors

Daniel J Fitzgerald; Philipp Berger; Christiane Schaffitzel; Kazuhiro Yamada; Timothy J Richmond; Imre Berger

doi:10.1038/nmeth983

journal article Nov 20, 2006

Protein complex expression by using multigene baculoviral vectors

Daniel J Fitzgerald Philipp Berger Christiane Schaffitzel

Kazuhiro Yamada Timothy J Richmond Imre Berger

Nature Methods Vol. 3 No. 12 pp. 1021-1032 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/nmeth983

Topics

No keywords indexed for this article. Browse by subject →

References

10

[1]

Berger, I., Fitzgerald, D.J. & Richmond, T.J. Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol. 22, 1583–1587 (2004). 10.1038/nbt1036

[2]

Benoit, R.M., Wilhelm, R.N., Scherer-Becker, D. & Ostermeier, C. An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids. Protein Expr. Pur. 45, 66–71 (2006). 10.1016/j.pep.2005.09.022

[3]

Tan, S., Kern, R.C. & Selleck, W. The pST44 polycistronic expression system for producing protein complexes in E.coli. Protein Expr. Pur. 40, 385–395 (2005). 10.1016/j.pep.2004.12.002

[4]

Tolia, N.H. & Joshua-Tor, L. Strategies for protein coexpression in Escherichia coli. Nat. Methods 3, 55–64 (2006). 10.1038/nmeth0106-55

[5]

A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae

Peter Uetz, Loic Giot, Gerard Cagney et al.

Nature 2000 10.1038/35001009

[6]

A Protein Interaction Map of Drosophila melanogaster

L. Giot, J. S. Bader, C. Brouwer et al.

Science 2003 10.1126/science.1090289

[7]

Simon, O., Williams, T., Caballero, P. & Lopez-Ferber, M. Dynamics of deletion genotypes in an experimental insect virus population. Proc. R. Soc. Lond. B 273, 783–790 (2006). 10.1098/rspb.2005.3394

[8]

Pijlman, G.B., de Vrij, J., van den End, F.J., Vlak, J.M. & Martens, D.E. Evaluation of baculovirus expression vectors with enhanced stability in continuous cascaded insect-cell bioreactors. Biotechnol. Bioeng. 87, 743–753 (2004). 10.1002/bit.20178

[9]

O'Reilly, D.R., Miller, L.K. & Luckov, V.A. eds. Baculovirus expression vectors. A laboratory manual. (Oxford University Press, New York, 1994).

[10]

Patterson, G., Day, R.N. & Piston, D. Fluorescent protein spectra. J. Cell Sci. 114, 837–838 (2001). 10.1242/jcs.114.5.837

Cited By

360

Structural and functional characterization of the Sin Nombre virus L protein

Kristina Meier, Sigurdur R. Thorkelsson · 2023

PLOS Pathogens

THUMPD3–TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates

Wen-qing Yang, Qing-Ping Xiong · 2021

Nucleic Acids Research

Free fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein

Christine Toelzer, Kapil Gupta · 2020

Science

JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Christian Albig, Chao Wang · 2019

Nature Communications

Efficient production of a mature and functional gamma secretase protease

Imran Khan, Sudarsan Krishnaswamy · 2018

Scientific Reports

The dynamic organization of fungal acetyl-CoA carboxylase

Moritz Hunkeler, Edward Stuttfeld · 2016

Nature Communications

Notch is a direct negative regulator of the DNA-damage response

Jelena Vermezovic, Marek Adamowicz · 2015

Nature Structural & Molecular B...

Crystal structures of IFT70/52 and IFT52/46 provide insight into intraflagellar transport B core complex assembly

Michael Taschner, Fruzsina Kotsis · 2014

The Journal of cell biology

High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system

Anja Basters, Lars Ketscher · 2012

BMC Biotechnology

Metrics

360

Citations

10

References

Details

Published: Nov 20, 2006
Vol/Issue: 3(12)
Pages: 1021-1032
License: View

Authors

Christiane Schaffitzel

Cite This Article

Daniel J Fitzgerald, Philipp Berger, Christiane Schaffitzel, et al. (2006). Protein complex expression by using multigene baculoviral vectors. Nature Methods, 3(12), 1021-1032. https://doi.org/10.1038/nmeth983

Protein complex expression by using multigene baculoviral vectors

You May Also Like