Biolistic transfection of neuronal cultures using a hand-held gene gun

John A O'Brien; Sarah C R Lummis

doi:10.1038/nprot.2006.145

journal article Aug 01, 2006

Biolistic transfection of neuronal cultures using a hand-held gene gun

John A O'Brien Sarah C R Lummis

Nature Protocols Vol. 1 No. 2 pp. 977-981 · Springer Science and Business Media LLC

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References

14

[1]

Klein, T.M., Wolf, E.D., Wu, R. & Sanford, J.C. High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327, 70–73 (1987). 10.1038/327070a0

[2]

Christou, P., McCabe, D.E. & Swain, W.F. Stable transformation of soybean callus by DNA-coated gold particles. Plant Physiol. 87, 671–674 (1988). 10.1104/pp.87.3.671

[3]

Lin, M. The gene gun: current applications in cutaneous gene therapy. Int. J. Dermatol. 39, 161–170 (2000). 10.1046/j.1365-4362.2000.00925.x

[4]

Yang, C.H. et al. Seeing the gene therapy: application of gene gun technique to transfect and decolour pigmented rat skin with human agouti signalling protein cDNA. Gene Ther. 11, 1033–1039 (2004). 10.1038/sj.gt.3302264

[5]

O'Brien, J.A. et al. Modification to the hand-held gene gun: improvements for in vitro biolistic transfection of organotypic neuronal tissue. J. Neurosci. Methods 112, 57–64 (2001). 10.1016/s0165-0270(01)00457-5

[6]

Jiao, S., Cheng, L., Wolff, J.A. & Yang, N.-S. Particle bombardment mediated gene transfer and expression in rat brain tissues. Biotechnology 11, 497–502 (1993).

[7]

Wirth, M.J. & Wahle, P. Biolistic transfection of organotypic cultures of rat visual cortex using a handheld device. J. Neurosci. Methods 125, 45–54 (2003). 10.1016/s0165-0270(03)00024-4

[8]

Gerber, H.-P., Dixit, V. & Ferrara, N. Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl2 and A1 in vascular endothelial cells. J. Biol. Chem. 273, 13313–13306 (1998). 10.1074/jbc.273.21.13313

[9]

Wellmann, H., Kaltschmidt, B. & Kaltschmidt, C. Optimized protocol for biolistic transfection of brain slices and dissociated cultured neurons with a hand-held gene gun. J. Neurosci. Methods 92, 55–64 (1999). 10.1016/s0165-0270(99)00094-1

[10]

Sanford, J.C., Smith, F.D. & Russell, J.A. Optimizing the biolistic process for different biological applications. Methods Enzymol. 217, 483–509 (1993). 10.1016/0076-6879(93)17086-k

[11]

Hargreaves, A.C., Lummis, S.C. & Taylor, C.W. Ca2+ permeability of cloned and native 5-hydroxytryptamine type-3 receptors. Mol. Pharmacol. 46, 1120–1126 (1994).

[12]

Stoppini, L., Buchs, P.A. & Muller, D. A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods 37, 173–182 (1991). 10.1016/0165-0270(91)90128-m

[13]

Shaw, G., Morse, S., Ararat, M. & Graham, F.L. Preferential transformations of human neuronal cells by human adenoviruses and the origin of HEK-293 cells. FASEB J. 16, 869–871 (2002). 10.1096/fj.01-0995fje

[14]

Wirth, M.J., Patz, S. & Wahle, P. Transcellular induction of neuropeptide Y expression by NT4 and BDNF. Proc. Natl. Acad. Sci. USA 102, 3064–3069 (2005). 10.1073/pnas.0404712102

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Published: Aug 01, 2006
Vol/Issue: 1(2)
Pages: 977-981
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John A O'Brien, Sarah C R Lummis (2006). Biolistic transfection of neuronal cultures using a hand-held gene gun. Nature Protocols, 1(2), 977-981. https://doi.org/10.1038/nprot.2006.145

Biolistic transfection of neuronal cultures using a hand-held gene gun

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