Analysis of apoptosis by propidium iodide staining and flow cytometry

Carlo Riccardi; Ildo Nicoletti

doi:10.1038/nprot.2006.238

journal article Aug 01, 2006

Analysis of apoptosis by propidium iodide staining and flow cytometry

Carlo Riccardi

Ildo Nicoletti

Nature Protocols Vol. 1 No. 3 pp. 1458-1461 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/nprot.2006.238

Topics

No keywords indexed for this article. Browse by subject →

References

16

[1]

Wyllie, A.H. Apoptosis: an overview. Br. Med. Bull. 53, 451–465 (1997). 10.1093/oxfordjournals.bmb.a011623

[2]

Arends, M.J., Morris, R.G. & Wyllie, A.H. Apoptosis. The role of the endonuclease. Am. J. Pathol. 136, 593–608 (1990).

[3]

Sellins, K.S. & Cohen, J.J. Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes. J. Immunol. 139, 3199–3206 (1987). 10.4049/jimmunol.139.10.3199

[4]

Gong, J., Traganos, F. & Darzynkiewicz, Z. A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal. Biochem. 218, 314–319 (1994). 10.1006/abio.1994.1184

[5]

Nunez, R. DNA measurement and cell cycle analysis by flow cytometry. Curr. Issues Mol. Biol. 3, 67–70 (2001).

[6]

Nicoletti, I., Migliorati, G., Pagliacci, M.C., Grignani, F. & Riccardi, C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139, 271–279 (1991). 10.1016/0022-1759(91)90198-o

[7]

Ormerod, M.G. Investigating the relationship between the cell cycle and apoptosis using flow cytometry. J. Immunol. Methods 265, 73–80 (2002). 10.1016/s0022-1759(02)00071-6

[8]

Darzynkiewicz, Z., Bedner, E. & Smolewski, P. Flow cytometry in analysis of cell cycle and apoptosis. Semin. Hematol. 38, 179–193 (2001). 10.1016/s0037-1963(01)90051-4

[9]

Telford, W.G., King, L.E. & Fraker, P.J. Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. J. Immunol. Methods 172, 1–16 (1994). 10.1016/0022-1759(94)90373-5

[10]

Wallen, C.A., Higashikubo, R. & Dethlefsen, L.A. Comparison of two flow cytometric assays for cellular RNA—acridine orange and propidium iodide. Cytometry 3, 155–160 (1982). 10.1002/cyto.990030303

[11]

Pollack, A. & Ciancio, G. Cell cycle phase-specific analysis of cell viability using Hoechst 33342 and propidium iodide after ethanol preservation. Methods Cell. Biol. 33, 19–24 (1990). 10.1016/s0091-679x(08)60508-7

[12]

Ormerod, M.G., Collins, M.K., Rodriguez-Tarduchy, G. & Robertson, D. Apoptosis in interleukin-3-dependent haemopoietic cells. Quantification by two flow cytometric methods. J. Immunol. Methods 153, 57–65 (1992). 10.1016/0022-1759(92)90305-d

[13]

Schmid, I., Uittenbogaart, C.H., Keld, B. & Giorgi, J.V. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods 170, 145–157 (1994). 10.1016/0022-1759(94)90390-5

[14]

Wyllie, A.H., Morris, R.G., Smith, A.L. & Dunlop, D. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J. Pathol. 142, 67–77 (1984). 10.1002/path.1711420112

[15]

Gavrieli, Y., Sherman, Y. & Ben-Sasson, S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119, 493–501 (1992). 10.1083/jcb.119.3.493

[16]

Telford, W.G., Komoriya, A. & Packard, B.Z. Detection of localized caspase activity in early apoptotic cells by laser scanning cytometry. Cytometry 47, 81–88 (2002). 10.1002/cyto.10052

Cited By

1,404

Multi-target miRNA Sponge for Pancreatic Ductal Adenocarcinoma: Simultaneous Targeting of miR-21, miR-155, and miR-18a

Mahdi Karimi, Nasrin Motamed · 2026

The Journal of Pharmacology and Exp...

Evidence for different seeding activities of misfolded tau in classical and rapidly progressive Alzheimer's disease

Matthias Schmitz, Katrin Thüne · 2025

Brain Pathology

Novel cyanochalcones as potential anticancer agents: apoptosis, cell cycle arrest, DNA binding, and molecular docking studies

Mohamed A. Ragheb, Hanan E. Abdelrashid · 2024

Journal of Biomolecular Structure a...

Ribosome Quality Control mitigates the cytotoxicity of ribosome collisions induced by 5-Fluorouracil

Susanta Chatterjee, Parisa Naeli · 2024

Nucleic Acids Research

Microbiota-derived tryptophan metabolites indole-3-lactic acid is associated with intestinal ischemia/reperfusion injury via positive regulation of YAP and Nrf2

Fang-Ling Zhang, Xiao-Wei Chen · 2023

Journal of Translational Medicine

Metal-Enhanced Fluorescent Carbon Quantum Dots via One-Pot Solid State Synthesis for Cell Imaging

Volkan Can, Bugra Onat · 2023

ACS Applied Bio Materials

Design, Synthesis, In Silico and In Vitro Studies of New Immunomodulatory Anticancer Nicotinamide Derivatives Targeting VEGFR-2

Reda G. Yousef, Wagdy M. Eldehna · 2022

Molecules

Formulation of Nanomicelles Loaded with Cannabidiol as a Platform for Neuroprotective Therapy

Yordan Yordanov, Denitsa Stefanova · 2022

Pharmaceutics

PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons

Wei-Tao Yan, Wen-Juan Zhao · 2022

Neural Regeneration Research

The remediation of nano-/microplastics from water

Marco Sarcletti, Hyoungwon Park · 2021

Materials Today

BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation

Drake S. Edwards, Rohin Maganti · 2020

Cell Reports

Discovery and antiproliferative evaluation of new quinoxalines as potential DNA intercalators and topoisomerase II inhibitors

Ibrahim H. Eissa, Ahmed M. Metwaly · 2019

Archiv der Pharmazie

The trypan blue cellular debris assay: a novel low-cost method for the rapid quantification of cell death

Paul F. Lebeau, Jack Chen · 2019

MethodsX

Tribenzyltin carboxylates as anticancer drug candidates: Effect on the cytotoxicity, motility and invasiveness of breast cancer cell lines

Theebaa Anasamy, Chun Keng Thy · 2017

European Journal of Medicinal Chemi...

Caspase-1 Inflammasome Activation Mediates Homocysteine-Induced Pyrop-Apoptosis in Endothelial Cells

Hang Xi, Yuling Zhang · 2016

Circulation Research

Hyperthermia Selectively Targets Human Papillomavirus in Cervical Tumors via p53-Dependent Apoptosis

Arlene L. Oei, Caspar M. van Leeuwen · 2015

Cancer Research

Artesunate induces ROS- and p38 MAPK-mediated apoptosis and counteracts tumor growthin vivoin embryonal rhabdomyosarcoma cells

Sara Beccafico, Giulio Morozzi · 2015

Carcinogenesis

Autophagy modulates endoplasmic reticulum stress-induced cell death in podocytes: A protective role

Yu-Chi Cheng, Jer-Ming Chang · 2014

Experimental Biology and Medicine

DAB2IP coordinates both PI3K-Akt and ASK1 pathways for cell survival and apoptosis

Daxing Xie, Crystal Gore · 2009

Proceedings of the National Academy...

Metrics

1,404

Citations

16

References

Details

Published: Aug 01, 2006
Vol/Issue: 1(3)
Pages: 1458-1461
License: View

Authors

C

Carlo Riccardi

Department of Medicine, Section of Pharmacology, University of Perugia, Piazza Severi 1, Perugia 06132, Italy

I

Ildo Nicoletti

Cite This Article

Carlo Riccardi, Ildo Nicoletti (2006). Analysis of apoptosis by propidium iodide staining and flow cytometry. Nature Protocols, 1(3), 1458-1461. https://doi.org/10.1038/nprot.2006.238

Analysis of apoptosis by propidium iodide staining and flow cytometry

You May Also Like