journal article Mar 01, 2007

A protocol for isolation and culture of human umbilical vein endothelial cells

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References
33
[1]
Ryan, J.W. & Ryan, U.S. Endothelial surface enzymes and the dynamic processing of plasma substrates. Int. Rev. Exp. Pathol. 26, 1–43 (1984). 10.1016/s0232-1513(84)80062-6
[2]
Kaiser, L. & Sparks, H.V. Jr . Endothelial cells. Not just a cellophane wrapper. Arch. Intern. Med. 147, 569–573 (1987). 10.1001/archinte.1987.00370030173034
[3]
Vane, J.R., Anggard, E.E . & Botting, R.M. Regulatory functions of the vascular endothelium. N. Engl. J. Med. 323, 27–36 (1990). 10.1056/nejm199007053230106
[4]
Lazo, J.S. Endothelial injury caused by antineoplastic agents. Biochem. Pharmacol. 35, 1919–1923 (1986). 10.1016/0006-2952(86)90720-3
[5]
Baudin, B. Toxicité endothéliale des chimiothérapies anticancéreuses. Sang Thrombose et Vaisseaux 7, 175–183 (1995).
[6]
Baudin, B., Bénéteau-Burnat, B. & Giboudeau, J. Cytotoxicity of amiodarone in cultured human endothelial cells. Cardiovasc. Drugs Ther. 10, 557–560 (1996). 10.1007/bf00050996
[7]
Mailloux, A. et al. Anticancer drugs induce necrosis of human endothelial cells involving both oncosis and apoptosis. Eur. J. Cell Biol. 80, 442–449 (2001). 10.1078/0171-9335-00171
[8]
Mailloux, A., Deslandes, B., Vaubourdolle, M. & Baudin, B. Captopril and enalaprilat decrease antioxidant defences in human endothelial cells and are unable to protect against apoptosis. Cell Biol. Intern. 27, 825–830 (2003). 10.1016/s1065-6995(03)00162-8
[9]
Mailloux, A., Bruneel, A., Vaubourdolle, M. & Baudin, B. Cyclosporin A but not estradiol can protect endothelial cells from etoposide-induced apoptosis. Endothelium 11, 141–149 (2004). 10.1080/10623320490512048
[10]
Bruneel, A. et al. Proteomic study of human umbilical vein endothelial cells in culture. Proteomics 3, 714–723 (2003). 10.1002/pmic.200300409
[11]
Bruneel, A. et al. Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis. Proteomics 5, 3876–3884 (2005). 10.1002/pmic.200401239
[12]
Pernet, P., Bruneel, A., Baudin, B. & Vaubourdolle, M. PHProteomicDB: a module for two-dimensional gel electrophoresis database creation on personal Web sites. Genomics Proteomics Bioinformatics 4, 134–136 (2006). 10.1016/s1672-0229(06)60024-7
[13]
Jaffe, E.A., Nachman, R.L., Becker, C.G. & Minick, C.R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J. Clin. Invest. 52, 2745–2756 (1973). 10.1172/jci107470
[14]
Ryan, U.S. & Ryan, J.W. Vital and functional activities of endothelial cells. in Pathobiology of the Endothelial Cells (eds. Nossel, H.L. & Vogel, H.J.) 455–469 (1982). 10.1016/b978-0-12-521980-8.50035-3
[15]
Goldsmith, J.C., McCormick, J.J. & Yen, A. Endothelial cell cycle kinetics. Changes in culture and correlation with endothelial properties. Lab. Invest. 51, 643–647 (1984).
[16]
Noveral, J.P., Mueller, S.N. & Levine, E.M. Release of angiotensin I-converting enzyme by endothelial cells in vitro. J. Cell. Physiol. 131, 1–5 (1987). 10.1002/jcp.1041310102
[17]
Dimmeler, S. & Zeiher, A.M. Endothelial cell apoptosis in angiogenesis and vessel regression. Circ. Res. 87, 434–439 (2000). 10.1161/01.res.87.6.434
[18]
Sawada, S. et al. Prostacyclin generation by cultured human vascular endothelial cells with reference to angiotensin I-converting enzyme. Jap. Circ. J. 50, 242–247 (1986). 10.1253/jcj.50.242
[19]
Okabe, T. et al. Induction by fibroblast growth factor of angiotensin-converting enzyme in vascular endothelial cells in vitro . Biochem. Biophys. Res. Commun. 145, 1211–1216 (1987). 10.1016/0006-291x(87)91566-x
[20]
Reinders, J.H., Vervoorn, R.C., Verweij, C.L., Van Mourik, J.A. & De Groot, P.G. Perturbation of cultured human vascular endothelial cells by phorbol ester or thrombin alters the cellular von Willebrand factor distribution. J. Cell. Physiol. 133, 79–87 (1987). 10.1002/jcp.1041330110
[21]
Nakache, M., Gaub, H.E., Schreiber, A.B. & Mac Connel, H.M. Topological and modulated distribution of surface markers on endothelial cells. Proc. Natl. Acad. Sci. USA 83, 2874–2878 (1986). 10.1073/pnas.83.9.2874
[22]
Wu, Q.Y. et al. Differential distribution of von Willebrand factor in endothelial cells. Comparison between normal pigs and pigs with von Willebrand disease. Arteriosclerosis 7, 47–54 (1987). 10.1161/01.atv.7.1.47
[23]
Baudin, B., Bérard, M., Carrier, J.L., Legrand, Y. & Drouet, L. Vascular origin determines angiotensin I-converting enzyme expression in endothelial cells. Endothelium 5, 73–84 (1997). 10.3109/10623329709044160
[24]
Miettinen, M., Holthofer, H., Lehto, V.-P., Miettinen, A. & Virtanen, I. Ulex europaeus I lectin as a marker for tumors derived from endothelial cells. Am. J. Clin. Pathol. 79, 32–36 (1983). 10.1093/ajcp/79.1.32
[25]
Carley, W.W., Niedbala, M.J. & Gerritsen, M.E. Isolation, cultivation, and partial characterization of microvascular endothelium derived from human lung. Am. J. Resp. Cell Mol. Biol. 7, 620–630 (1992). 10.1165/ajrcmb/7.6.620
[26]
Gorman, L., Mercer, L.-P. & Hennig, B. Growth requirements of endothelial cells in culture: variations in serum and amino acid concentrations. Nutrition 12, 266–270 (1996). 10.1016/s0899-9007(96)90854-0
[27]
Seeger, J.M. & Klingman, N. Improved endothelial cell seeding with cultured cells and fibronectin-coated grafts. J. Surg. Res. 38, 641–647 (1985). 10.1016/0022-4804(85)90087-3
[28]
Balconi, G., Pietra, A., Busacca, M., De Gaetano, G. & Dejana, E. Success rate of primary human endothelial cell culture from umbilical cords is influenced by maternal and fetal factors and interval from delivery. In Vitro 19, 807–810 (1983). 10.1007/bf02618159
[29]
Mano, Y., Sawasaki, Y., Takahashi, K. & Goto, T. Cultivation of arterial endothelial cells from human umbilical cord. Experientia 39, 1144–1146 (1983). 10.1007/bf01943152
[30]
Hirschberg, H., Bergh, O.J. & Thorsby, E. Antigen-presenting properties of human vascular endothelial cells. J. Exp. Med. 152, 249–255 (1980). 10.1084/jem.152.2.249
[31]
Schor, A.M., Schor, S.L. & Allen, T.D. The synthesis of sub-endothelial matrix by bovine aortic endothelial cells in culture. Tissue Cell 16, 677–691 (1984). 10.1016/0040-8166(84)90002-8
[32]
Ishisaki, A., Hayashi, H., Li, A.-J. & Imamura, T. Human umbilical vein endothelium-derived cells retain potential to differentiate into smooth muscle-like cells. J. Biol. Chem. 278, 1303–1309 (2003). 10.1074/jbc.m207329200
[33]
Baudin, B., Bénéteau-Burnat, B., Baumann, F.C. & Giboudeau, J. A reliable radiometric assay for the determination of angiotensin I-converting enzyme activity in urines. J. Clin. Chem. Clin. Biochem. 28, 857–861 (1990).
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Published
Mar 01, 2007
Vol/Issue
2(3)
Pages
481-485
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Cite This Article
Bruno Baudin, Arnaud Bruneel, Nelly Bosselut, et al. (2007). A protocol for isolation and culture of human umbilical vein endothelial cells. Nature Protocols, 2(3), 481-485. https://doi.org/10.1038/nprot.2007.54
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