Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis

AbstractWriting artificial logic and dynamic function into complex cellular background to achieve desired phenotypes or improved outputs calls for the development of new genetic tools as well as their innovative use. In this study, we present a sensor-regulator and RNAi-based bifunctional dynamic control network that can provide simultaneous upregulation and downregulation of cellular metabolism for engineered biosynthesis. The promoter-regulator-mediated upregulation function and its transduced downregulation function through RNAi are systematically verified and characterized. We apply this dynamic control network to regulate the phosphoenolpyruvate metabolic node in Escherichia coli and achieve autonomous distribution of carbon flux between its native metabolism and the engineered muconic acid biosynthetic pathway. This allows muconic acid biosynthesis to reach 1.8 g L−1. This study also suggests the circumstances where dynamic control approaches are likely to take effects.

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Details

Published: Aug 02, 2018
Vol/Issue: 9(1)
License: View

Authors

Liaoning University of Traditional Chinese Medicine

Y

Yifei Wu

Technology R&D Department Fujian Longking Co., Ltd. Longyan 364000 China

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People’s Republic of China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, People’s Republic of China

Q

Qipeng Yuan

Cite This Article

Yaping Yang, Yuheng Lin, Jian Wang, et al. (2018). Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05466-0

Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis

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