Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration

David Jäckel; Douglas J. Bakkum; Thomas L. Russell; Jan Müller; Miloš Radivojević; Urs Frey; Felix Franke; Andreas Hierlemann

doi:10.1038/s41598-017-00981-4

journal article Open Access Apr 20, 2017

Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration

David Jäckel Douglas J. Bakkum Thomas L. Russell Jan Müller

Miloš Radivojević Urs Frey

Felix Franke Andreas Hierlemann

Scientific Reports Vol. 7 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/s41598-017-00981-4

Abstract

AbstractWe present a novel, all-electric approach to record and to precisely control the activity of tens of individual presynaptic neurons. The method allows for parallel mapping of the efficacy of multiple synapses and of the resulting dynamics of postsynaptic neurons in a cortical culture. For the measurements, we combine an extracellular high-density microelectrode array, featuring 11’000 electrodes for extracellular recording and stimulation, with intracellular patch-clamp recording. We are able to identify the contributions of individual presynaptic neurons - including inhibitory and excitatory synaptic inputs - to postsynaptic potentials, which enables us to study dendritic integration. Since the electrical stimuli can be controlled at microsecond resolution, our method enables to evoke action potentials at tens of presynaptic cells in precisely orchestrated sequences of high reliability and minimum jitter. We demonstrate the potential of this method by evoking short- and long-term synaptic plasticity through manipulation of multiple synaptic inputs to a specific neuron.

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Published: Apr 20, 2017
Vol/Issue: 7(1)
License: View

Authors

Swiss Pediatric Respiratory Research Group, Department of Pediatrics, University Hospital of Zürich, Zürich; Department of Pediatrics, University Hospital of Berne; and Department of Social and Preventive Medicine, University of Berne, Berne, Switzerland

Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland

Cite This Article

David Jäckel, Douglas J. Bakkum, Thomas L. Russell, et al. (2017). Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-00981-4

Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration

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