In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia

Toru Yamashita; Jingwei Shang; Yumiko Nakano; Ryuta Morihara; Kota Sato; Mami Takemoto; Nozomi Hishikawa; Yasuyuki Ohta

doi:10.1038/s41598-019-47482-0

journal article Open Access Jul 29, 2019

In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia

Toru Yamashita Jingwei Shang

Yumiko Nakano Ryuta Morihara

Kota Sato

Mami Takemoto Nozomi Hishikawa Yasuyuki Ohta

Scientific Reports Vol. 9 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/s41598-019-47482-0

Abstract

AbstractThe therapeutic effect of in vivo direct reprogramming on ischemic stroke has not been evaluated. In the present study, a retroviral solution (1.5–2.0 × 107 /ul) of mock pMX-GFP (n = 13) or pMX-Ascl1/Sox2/NeuroD1 (ASN) (n = 14) was directly injected into the ipsilateral striatum and cortex 3 days after 30 min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.

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Published: Jul 29, 2019
Vol/Issue: 9(1)
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Toru Yamashita