Pre-erythrocytic antibody profiles induced by controlled human malaria infections in healthy volunteers under chloroquine prophylaxis

Philip L. Felgner; Meta Roestenberg; Li Liang; Christopher Hung; Aarti Jain; Jozelyn Pablo; Rie Nakajima-Sasaki; Douglas Molina; Karina Teelen; Cornelus C. Hermsen; Robert Sauerwein

doi:10.1038/srep03549

journal article Open Access Dec 19, 2013

Pre-erythrocytic antibody profiles induced by controlled human malaria infections in healthy volunteers under chloroquine prophylaxis

Philip L. Felgner Meta Roestenberg

Li Liang

Christopher Hung Aarti Jain Jozelyn Pablo Rie Nakajima-Sasaki Douglas Molina Karina Teelen Cornelus C. Hermsen Robert Sauerwein

Scientific Reports Vol. 3 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep03549

Abstract

AbstractComplete sterile protection to Plasmodium falciparum (Pf) infection mediated by pre-erythrocytic immunity can be experimentally induced under chloroquine prophylaxis, through immunization with sporozoites from infected mosquitoes' bites (CPS protocol). To characterize the profile of CPS induced antibody (Ab) responses, we developed a proteome microarray containing 809 Pf antigens showing a distinct Ab profile with recognition of antigens expressed in pre-erythrocytic life-cycle stages. In contrast, plasma from naturally exposed semi-immune individuals from Kenya was skewed toward antibody reactivity against asexual blood stage antigens. CPS-immunized and semi-immune individuals generated antibodies against 192 and 202 Pf antigens, respectively, but only 60 antigens overlapped between the two groups. Although the number of reactive antigens varied between the CPS-immunized individuals, all volunteers reacted strongly against the pre-erythrocytic antigens circumsporozoite protein (CSP) and liver stage antigen 1 (LSA1). Well classified merozoite and erythrocytic antigens were strongly reactive in semi-immune individuals but lacking in the CPS immunized group. These data show that the antibody profile of CPS-immunized and semi-immune groups have quite distinct profiles reflecting their protective immunity; antibodies from CPS immunized individuals react strongly against pre-erythrocytic while semi-immune individuals mainly react against erythrocytic antigens.

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References

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Published: Dec 19, 2013
Vol/Issue: 3(1)
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Cite This Article

Philip L. Felgner, Meta Roestenberg, Li Liang, et al. (2013). Pre-erythrocytic antibody profiles induced by controlled human malaria infections in healthy volunteers under chloroquine prophylaxis. Scientific Reports, 3(1). https://doi.org/10.1038/srep03549

Pre-erythrocytic antibody profiles induced by controlled human malaria infections in healthy volunteers under chloroquine prophylaxis

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