Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growth

Michiko Shigyo; Tomoharu Kuboyama; Yusuke Sawai; Masahito Tada-Umezaki; Chihiro Tohda

doi:10.1038/srep12055

journal article Open Access Jul 14, 2015

Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growth

Michiko Shigyo Tomoharu Kuboyama Yusuke Sawai Masahito Tada-Umezaki Chihiro Tohda

Scientific Reports Vol. 5 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep12055

Abstract

AbstractVimentin, an intermediate filament protein, is generally recognised as an intracellular protein. Previously, we reported that vimentin was secreted from astrocytes and promoted axonal growth. The effect of extracellular vimentin in neurons was a new finding, but its signalling pathway was unknown. In this study, we aimed to determine the signalling mechanism of extracellular vimentin that facilitates axonal growth. We first identified insulin-like growth factor 1 receptor (IGF1R) as a receptor that is highly phosphorylated by vimentin stimulation. IGF1R blockades diminished vimentin- or IGF1-induced axonal growth in cultured cortical neurons. IGF1, IGF2 and insulin were not detected in the neuron culture medium after vimentin treatment. The combined drug affinity responsive target stability method and western blotting analysis showed that vimentin and IGF1 interacted with IGF1R directly. In addition, immunoprecipitation and western blotting analyses confirmed that recombinant IGF1R bound to vimentin. The results of a molecular dynamics simulation revealed that C-terminal residues (residue number 330-407) in vimentin are the most appropriate binding sites with IGF1R. Thus, extracellular vimentin may be a novel ligand of IGF1R that promotes axonal growth in a similar manner to IGF1. Our results provide novel findings regarding the role of extracellular vimentin and IGF1R in axonal growth.

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Details

Published: Jul 14, 2015
Vol/Issue: 5(1)
License: View

Authors

Division of Neuromedical Science, Department of Bioscience, and ‡Division of Kampo Pharmaceutics, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan

Y

Yusuke Sawai

M

Masahito Tada-Umezaki

C

Chihiro Tohda

Section of Neuromedical Science, Division of Bioscience Institute of Natural Medicine, University of Toyama Toyama Japan

Cite This Article

Michiko Shigyo, Tomoharu Kuboyama, Yusuke Sawai, et al. (2015). Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growth. Scientific Reports, 5(1). https://doi.org/10.1038/srep12055

Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growth

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