PPARγ stimulates expression of L-type amino acid and taurine transporters in human placentas: the evidence of PPARγ regulating fetal growth

Zhaoguang Chen; Ping He; Xiaoying Ding; Ying Huang; Hang Gu; Xin Ni

doi:10.1038/srep12650

journal article Open Access Jul 31, 2015

PPARγ stimulates expression of L-type amino acid and taurine transporters in human placentas: the evidence of PPARγ regulating fetal growth

Zhaoguang Chen Ping He

Xiaoying Ding

Ying Huang

Hang Gu Xin Ni

Scientific Reports Vol. 5 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep12650

Abstract

AbstractPlacental amino acid transporters and peroxisome proliferator-activated receptors (PPARs) have been implicated to placental development and therefore regulation of fetal growth. We analyzed the correlation between the expression of amino acid transporters and PPARs and investigated whether PPARs control the expression of amino acid transporters in placentas. It was found that protein expression of PPARγ and L-type amino acid transporter 1(LAT1) and 2 (LAT2) was decreased in small-for-gestational-age (SGA) placentas. LAT1, LAT2 and taurine transporter (TAUT) expression correlated to PPARγ level and birth weight. In cultured placental cells, PPARγ agonist stimulated LAT1 and LAT2 and TAUT, which was reversed by PPARγ siRNA. PPARγ up-regulation of LAT1 and TAUT was through specificity protein 1 (Sp-1) while stimulation of LAT2 expression was via induction of gene transcription. Our data suggest that PPARγ, SP-1, LAT1 and LAT2 in placentas are involved in control of fetal growth. PPARγ signaling pathway may be the therapeutic target for intrauterine growth restriction.

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Kenneth J. Livak, Thomas D. Schmittgen

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Details

Published: Jul 31, 2015
Vol/Issue: 5(1)
License: View

Authors

Structural Biology and Cancer Drug Discovery Program, Lombardi Cancer Center and Department of Oncology, Georgetown University Medical Center, 3970 Reservoir Road, Washington, D.C. 20007, and Laboratory of Medicinal Chemistry, National Cancer Institute, FCRDC, Building 376, Room 207, Fredrick, Maryland 21702

Department of the Hospital Director, National Center for Children’s Health, Beijing Children’s Hospital Affiliated to Capital Medical University, Beijing, China

Cite This Article

Zhaoguang Chen, Ping He, Xiaoying Ding, et al. (2015). PPARγ stimulates expression of L-type amino acid and taurine transporters in human placentas: the evidence of PPARγ regulating fetal growth. Scientific Reports, 5(1). https://doi.org/10.1038/srep12650

PPARγ stimulates expression of L-type amino acid and taurine transporters in human placentas: the evidence of PPARγ regulating fetal growth

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