Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation

Giuseppe Mazza; Krista Rombouts; Andrew Rennie Hall; Luca Urbani; Tu Vinh Luong; Walid Al-Akkad; Lisa Longato; David Brown; Panagiotis Maghsoudlou; Amar P. Dhillon; Barry Fuller; Brian Davidson; Kevin Moore; Dipok Dhar; Paolo De Coppi; Massimo Malago; Massimo Pinzani

doi:10.1038/srep13079

journal article Open Access Aug 07, 2015

Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation

Giuseppe Mazza

Krista Rombouts Andrew Rennie Hall Luca Urbani Tu Vinh Luong Walid Al-Akkad Lisa Longato David Brown

Panagiotis Maghsoudlou Amar P. Dhillon Barry Fuller Brian Davidson

Kevin Moore

Dipok Dhar Paolo De Coppi

Massimo Malago Massimo Pinzani

Scientific Reports Vol. 5 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep13079

Abstract

AbstractLiver synthetic and metabolic function can only be optimised by the growth of cells within a supportive liver matrix. This can be achieved by the utilisation of decellularised human liver tissue. Here we demonstrate complete decellularization of whole human liver and lobes to form an extracellular matrix scaffold with a preserved architecture. Decellularized human liver cubic scaffolds were repopulated for up to 21 days using human cell lines hepatic stellate cells (LX2), hepatocellular carcinoma (Sk-Hep-1) and hepatoblastoma (HepG2), with excellent viability, motility and proliferation and remodelling of the extracellular matrix. Biocompatibility was demonstrated by either omental or subcutaneous xenotransplantation of liver scaffold cubes (5 × 5 × 5 mm) into immune competent mice resulting in absent foreign body responses. We demonstrate decellularization of human liver and repopulation with derived human liver cells. This is a key advance in bioartificial liver development.

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References

Details

Published: Aug 07, 2015
Vol/Issue: 5(1)
License: View

Authors

Panagiotis Maghsoudlou

Hepatobiliary and Pancreatic Surgery Unit, The Royal Free Hospital, London, UK

K

Kevin Moore

Department of Primary Industries Tamworth Agricultural Institute, Calala, NSW 2340, Australia

Hepato-pancreatic-biliary and Liver Transplantation Surgery, Royal Free Hospital Campus, University College London, London NW3 2QG, UK

M

Massimo Pinzani

Cite This Article

Giuseppe Mazza, Krista Rombouts, Andrew Rennie Hall, et al. (2015). Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation. Scientific Reports, 5(1). https://doi.org/10.1038/srep13079

Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation

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