A comparative study for the inactivation of multidrug resistance bacteria using dielectric barrier discharge and nano-second pulsed plasma

Naresh Kumar; Dae Hoon Park; Maksudbek Yusupov; Erik C. Neyts; Christof C. W. Verlackt; Annemie Bogaerts; Min Ho Kang; Han Sup Uhm; Eun Ha Choi; Pankaj Attri

doi:10.1038/srep13849

journal article Open Access Sep 09, 2015

A comparative study for the inactivation of multidrug resistance bacteria using dielectric barrier discharge and nano-second pulsed plasma

Naresh Kumar

Dae Hoon Park Maksudbek Yusupov Erik C. Neyts

Christof C. W. Verlackt Annemie Bogaerts

Min Ho Kang Han Sup Uhm Eun Ha Choi Pankaj Attri

Scientific Reports Vol. 5 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep13849

Abstract

AbstractBacteria can be inactivated through various physical and chemical means and these have always been the focus of extensive research. To further improve the methodology for these ends, two types of plasma systems were investigated: nano-second pulsed plasma (NPP) as liquid discharge plasma and an Argon gas-feeding dielectric barrier discharge (Ar-DBD) as a form of surface plasma. To understand the sterilizing action of these two different plasma sources, we performed experiments with Staphylococcus aureus (S. aureus) bacteria (wild type) and multidrug resistant bacteria (Penicillum-resistant, Methicillin-resistant and Gentamicin-resistant). We observed that both plasma sources can inactivate both the wild type and multidrug-resistant bacteria to a good extent. Moreover, we observed a change in the surface morphology, gene expression and β-lactamase activity. Furthermore, we used X-ray photoelectron spectroscopy to investigate the variation in functional groups (C-H/C-C, C-OH and C=O) of the peptidoglycan (PG) resulting from exposure to plasma species. To obtain atomic scale insight in the plasma-cell interactions and support our experimental observations, we have performed molecular dynamics simulations to study the effects of plasma species, such as OH, H2O2, O, O3, as well as O2 and H2O, on the dissociation/formation of above mentioned functional groups in PG.

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Published: Sep 09, 2015
Vol/Issue: 5(1)
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Authors

Department of Chemistry PLASMANT Research Group NANOLab Center of Excellence University of Antwerp Universiteitsplein 1 2610 Wilrijk Belgium

C

Christof C. W. Verlackt

A

Annemie Bogaerts

Research Group PLASMANT; Department of Chemistry; University of Antwerp; B-2610 Wilrijk-Antwerp; Belgium

Plasma Bioscience Research Center, Kwangwoon University, Seoul 139701, Korea

Cite This Article

Naresh Kumar, Dae Hoon Park, Maksudbek Yusupov, et al. (2015). A comparative study for the inactivation of multidrug resistance bacteria using dielectric barrier discharge and nano-second pulsed plasma. Scientific Reports, 5(1). https://doi.org/10.1038/srep13849

A comparative study for the inactivation of multidrug resistance bacteria using dielectric barrier discharge and nano-second pulsed plasma

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