The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein

Ying Fang; Feilong Zhang; Aiguo Zhao; Wenxia Zhang; Min Zhang; Lin Liu

doi:10.1038/srep19756

journal article Open Access Jan 22, 2016

The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein

Ying Fang

Feilong Zhang

Aiguo Zhao Wenxia Zhang

Min Zhang

Lin Liu

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep19756

Abstract

AbstractTetrapyrrole biosynthesis is an essential and tightly regulated process and glutamyl-tRNA reductase (GluTR) is a key target for multiple regulatory factors at the post-translational level. By binding to the thylakoid membrane protein FLUORESCENT (FLU) or the soluble stromal GluTR-binding protein (GBP), the activity of GluTR is down- or up-regulated. Here, we reconstructed a ternary complex composed of the C-terminal tetratricopepetide-repeat domain of FLU, GBP and GluTR, crystallized and solved the structure of the complex at 3.2 Å. The overall structure resembles the shape of merged two binary complexes as previously reported and shows a large conformational change within GluTR. We also demonstrated that GluTR binds tightly with GBP but does not bind to GSAM under the same condition. These findings allow us to suggest a biological role of the ternary complex for the regulation of plant GluTR.

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Details

Published: Jan 22, 2016
Vol/Issue: 6(1)
License: View

Authors

Y

Ying Fang

Department of Gastroenterology The Affiliated Children's Hospital of Xi'an Jiaotong University Xi'an Shaanxi Province China

State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics, Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China; College of Earth Sciences University of Chinese Academy of Sciences Beijing China

M

Min Zhang

Huanggang Normal University

L

Lin Liu

Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA.

Cite This Article

Ying Fang, Feilong Zhang, Aiguo Zhao, et al. (2016). The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein. Scientific Reports, 6(1). https://doi.org/10.1038/srep19756

The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein

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