Label-free Electrochemiluminescent Immunosensor for Detection of Prostate Specific Antigen based on Aminated Graphene Quantum Dots and Carboxyl Graphene Quantum Dots

Dan Wu; Yixin Liu; Yaoguang Wang; Lihua Hu; Hongmin Ma; Guoqin Wang; Qin Wei

doi:10.1038/srep20511

journal article Open Access Feb 04, 2016

Label-free Electrochemiluminescent Immunosensor for Detection of Prostate Specific Antigen based on Aminated Graphene Quantum Dots and Carboxyl Graphene Quantum Dots

Dan Wu

Yixin Liu

Yaoguang Wang Lihua Hu

Hongmin Ma Guoqin Wang Qin Wei

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep20511

Abstract

AbstractProstate-specific antigen (PSA) was used as the model, an ultrasensitive label-free electrochemiluminescent immunosensor was developed based on graphene quantum dots. Au/Ag-rGO was sythsized and used as electrode material to load a great deal of graphene quantum dots due to the large surface area and excellent electron conductivity. After aminated graphene quantum dots and acarboxyl graphene quantum dots were modified onto the electrode, the ECL intensity was much high using K2S2O8 as coreactant. Then, antibody of PSA was immobilized on the surface of modified electrode surface through the adsorption of Au/Ag toward proteins, leading to the decrease of the ECL intensity. As proven by ECL spectra test and electrochemical impedance spectroscopy (EIS) analysis, the fabrication process of the immunosensor is successful. Under the optimal conditions, the ECL intensity decreased linearly with the logarithm of PSA concentration in the range of 1 pg/mL ~ 10 ng/mL. The detection limit achieved is 0.29 pg/mL. The immunosensor results were validated through the detection of PSA in serum samples with satisfactory results. Due to excellent stability, high sensitivity, acceptable repeatability and selectivity, the immunosensor has promising applications in disease and drug analysis.

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Details

Published: Feb 04, 2016
Vol/Issue: 6(1)
License: View

Authors

School of Chemistry and Chemical Engineering South China University of Technology Guangzhou Guangdong 510640 P.R. China

H

Hongmin Ma

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong School of Chemistry and Chemical Engineering University of Jinan Jinan 250022 China

Cite This Article

Dan Wu, Yixin Liu, Yaoguang Wang, et al. (2016). Label-free Electrochemiluminescent Immunosensor for Detection of Prostate Specific Antigen based on Aminated Graphene Quantum Dots and Carboxyl Graphene Quantum Dots. Scientific Reports, 6(1). https://doi.org/10.1038/srep20511

Label-free Electrochemiluminescent Immunosensor for Detection of Prostate Specific Antigen based on Aminated Graphene Quantum Dots and Carboxyl Graphene Quantum Dots

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