Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography

Nivedh Manohar; Francisco J. Reynoso; Parmeswaran Diagaradjane; Sunil Krishnan; Sang Hyun Cho

doi:10.1038/srep22079

journal article Open Access Feb 25, 2016

Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography

Nivedh Manohar Francisco J. Reynoso

Parmeswaran Diagaradjane Sunil Krishnan Sang Hyun Cho

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep22079

Abstract

AbstractX-ray fluorescence computed tomography (XFCT) is a technique that can identify, quantify and locate elements within objects by detecting x-ray fluorescence (characteristic x-rays) stimulated by an excitation source, typically derived from a synchrotron. However, the use of a synchrotron limits practicality and accessibility of XFCT for routine biomedical imaging applications. Therefore, we have developed the ability to perform XFCT on a benchtop setting with ordinary polychromatic x-ray sources. Here, we report our postmortem study that demonstrates the use of benchtop XFCT to accurately image the distribution of gold nanoparticles (GNPs) injected into a tumor-bearing mouse. The distribution of GNPs as determined by benchtop XFCT was validated using inductively coupled plasma mass spectrometry. This investigation shows drastically enhanced sensitivity and specificity of GNP detection and quantification with benchtop XFCT, up to two orders of magnitude better than conventional x-ray CT. The results also reaffirm the unique capabilities of benchtop XFCT for simultaneous determination of the spatial distribution and concentration of nonradioactive metallic probes, such as GNPs, within the context of small animal imaging. Overall, this investigation identifies a clear path toward in vivo molecular imaging using benchtop XFCT techniques in conjunction with GNPs and other metallic probes.

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References

Details

Published: Feb 25, 2016
Vol/Issue: 6(1)
License: View

Authors

N

Nivedh Manohar

Department of Radiation Physics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA

F

Francisco J. Reynoso

Department of Radiation Physics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA

P

Parmeswaran Diagaradjane

S

Sunil Krishnan

2M.D. Anderson Cancer Center, Houston, TX

S

Sang Hyun Cho

Department of Radiation Physics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA; Department of Imaging Physics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA

Cite This Article

Nivedh Manohar, Francisco J. Reynoso, Parmeswaran Diagaradjane, et al. (2016). Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography. Scientific Reports, 6(1). https://doi.org/10.1038/srep22079

Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography

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