The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise

Riki Ogasawara; Satoshi Fujita; Troy A. Hornberger; Yu Kitaoka; Yuhei MAKANAE; Koichi Nakazato; Ishii Naokata

doi:10.1038/srep31142

journal article Open Access Aug 09, 2016

The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise

Riki Ogasawara

Satoshi Fujita

Troy A. Hornberger

Yu Kitaoka Yuhei MAKANAE Koichi Nakazato Ishii Naokata

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep31142

Abstract

Abstract
Resistance exercise (RE) activates signalling by the mammalian target of rapamycin (mTOR) and it has been suggested that rapamycin-sensitive mTOR signalling controls RE-induced changes in protein synthesis, ribosome biogenesis, autophagy and the expression of peroxisome proliferator gamma coactivator 1 alpha (PGC-1α). However, direct evidence to support the aforementioned relationships is lacking. Therefore, in this study, we investigated the role of rapamycin-sensitive mTOR in the RE-induced activation of muscle protein synthesis, ribosome biogenesis, PGC-1α expression and hypertrophy. The results indicated that the inhibition of rapamycin-sensitive mTOR could prevent the induction of ribosome biogenesis by RE, but it only partially inhibited the activation of muscle protein synthesis. Likewise, the inhibition of rapamycin-sensitive mTOR only partially blocked the hypertrophic effects of chronic RE. Furthermore, both acute and chronic RE promoted an increase in PGC-1α expression and these alterations were not affected by the inhibition of rapamycin-sensitive mTOR. Combined, the results from this study not only establish that rapamycin-sensitive mTOR plays an important role in the RE-induced activation of protein synthesis and the induction of hypertrophy, but they also demonstrate that additional (rapamycin-sensitive mTOR-independent) mechanisms contribute to these fundamentally important events.

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Published: Aug 09, 2016
Vol/Issue: 6(1)
License: View

Authors

R

Riki Ogasawara

Healthy Food Science Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin, United States

Ritsumeikan Global Innovation Research Organization, Ritsumeikan University; Faculty of Sport and Health Science, Ritsumeikan University

Cite This Article

Riki Ogasawara, Satoshi Fujita, Troy A. Hornberger, et al. (2016). The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise. Scientific Reports, 6(1). https://doi.org/10.1038/srep31142

The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise

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