M-DNA/Transition Metal Dichalcogenide Hybrid Structure-based Bio-FET sensor with Ultra-high Sensitivity

Hyung-Youl Park; Sreekantha Reddy Dugasani; Dong-Ho Kang; Gwangwe Yoo; Jinok Kim; Bramaramba Gnapareddy; Jaeho Jeon; MinWoo Kim; Young Jae Song; Sungjoo Lee; Jonggon Heo; Young Jin Jeon; Sung Ha Park; Jin-Hong Park

doi:10.1038/srep35733

journal article Open Access Oct 24, 2016

M-DNA/Transition Metal Dichalcogenide Hybrid Structure-based Bio-FET sensor with Ultra-high Sensitivity

Hyung-Youl Park

Sreekantha Reddy Dugasani Dong-Ho Kang Gwangwe Yoo Jinok Kim Bramaramba Gnapareddy Jaeho Jeon

MinWoo Kim

Young Jae Song

Sungjoo Lee

Jonggon Heo Young Jin Jeon Sung Ha Park Jin-Hong Park

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep35733

Abstract

AbstractHere, we report a high performance biosensor based on (i) a Cu2+-DNA/MoS2 hybrid structure and (ii) a field effect transistor, which we refer to as a bio-FET, presenting a high sensitivity of 1.7 × 103 A/A. This high sensitivity was achieved by using a DNA nanostructure with copper ions (Cu2+) that induced a positive polarity in the DNA (receptor). This strategy improved the detecting ability for doxorubicin-like molecules (target) that have a negative polarity. Very short distance between the biomolecules and the sensor surface was obtained without using a dielectric layer, contributing to the high sensitivity. We first investigated the effect of doxorubicin on DNA/MoS2 and Cu2+-DNA/MoS2 nanostructures using Raman spectroscopy and Kelvin force probe microscopy. Then, we analyzed the sensing mechanism and performance in DNA/MoS2- and Cu2+-DNA/MoS2-based bio-FETs by electrical measurements (ID-VG at various VD) for various concentrations of doxorubicin. Finally, successful operation of the Cu2+-DNA/MoS2 bio-FET was demonstrated for six cycles (each cycle consisted of four steps: 2 preparation steps, a sensing step, and an erasing step) with different doxorubicin concentrations. The bio-FET showed excellent reusability, which has not been achieved previously in 2D biosensors.

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Details

Published: Oct 24, 2016
Vol/Issue: 6(1)
License: View

Authors

H

Hyung-Youl Park

Department of Orthopaedic Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

S

Sreekantha Reddy Dugasani

Bramaramba Gnapareddy

Cite This Article

Hyung-Youl Park, Sreekantha Reddy Dugasani, Dong-Ho Kang, et al. (2016). M-DNA/Transition Metal Dichalcogenide Hybrid Structure-based Bio-FET sensor with Ultra-high Sensitivity. Scientific Reports, 6(1). https://doi.org/10.1038/srep35733

M-DNA/Transition Metal Dichalcogenide Hybrid Structure-based Bio-FET sensor with Ultra-high Sensitivity

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