The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development

Masanori Kobayashi; Shigeru Oshima; Chiaki Maeyashiki; Yoichi Nibe; Kana Otsubo; Yu Matsuzawa; Yasuhiro Nemoto; Takashi Nagaishi; Ryuichi Okamoto; Kiichiro Tsuchiya; Tetsuya Nakamura; Mamoru Watanabe

doi:10.1038/srep36780

journal article Open Access Nov 10, 2016

The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development

Masanori Kobayashi Shigeru Oshima Chiaki Maeyashiki Yoichi Nibe Kana Otsubo Yu Matsuzawa Yasuhiro Nemoto

Takashi Nagaishi Ryuichi Okamoto

Kiichiro Tsuchiya Tetsuya Nakamura Mamoru Watanabe

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

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Abstract

AbstractUbiquitination is a crucial post-translational modification; however, the functions of ubiquitin-coding genes remain unclear. UBA52 encodes a fusion protein comprising ubiquitin at the N-terminus and ribosomal protein L40 (RPL40) at the C-terminus. Here we showed that Uba52-deficient mice die during embryogenesis. UBA52-deficient cells exhibited normal levels of total ubiquitin. However, UBA52-deficient cells displayed decreased protein synthesis and cell-cycle arrest. The overexpression of UBA52 ameliorated the cell-cycle arrest caused by UBA52 deficiency. Surprisingly, RPL40 expression itself is insufficient to regulate cyclin D expression. The cleavage of RPL40 from UBA52 was required for maintaining protein synthesis. Furthermore, we found that RPL40 formed a ribosomal complex with ubiquitin cleaved from UBA52. UBA52 supplies RPL40 and ubiquitin simultaneously to the ribosome. Our study demonstrated that the ubiquitin-coding gene UBA52 is not just an ubiquitin supplier to the ubiquitin pool but is also a regulator of the ribosomal protein complex. These findings provide novel insights into the regulation of ubiquitin-dependent translation and embryonic development.

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Details

Published: Nov 10, 2016
Vol/Issue: 6(1)
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Authors

Graduate School of Information Science, University of Hyogo, 7-1-28 minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan

TMDU Advanced Research Institute Tokyo Medical and Dental University Tokyo Japan

Cite This Article

Masanori Kobayashi, Shigeru Oshima, Chiaki Maeyashiki, et al. (2016). The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development. Scientific Reports, 6(1). https://doi.org/10.1038/srep36780

The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development

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