Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments

Hongfei Ge; Anyong Yu; Jingyu Chen; Jichao Yuan; Yi Yin; Wangsheng Duanmu; Liang Tan; Yang Yang; Chuan Lan; Weixiang Chen; Hua Feng; Rong Hu

doi:10.1038/srep37681

journal article Open Access Nov 22, 2016

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments

Hongfei Ge

Anyong Yu Jingyu Chen

Jichao Yuan

Yi Yin

Wangsheng Duanmu Liang Tan

Yang Yang

Chuan Lan Weixiang Chen Hua Feng

Rong Hu

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep37681

Abstract

AbstractThe recruitment of neural stem/progenitor cells (NSPCs) for brain restoration after injury is a promising regenerative therapeutic strategy. This strategy involves enhancing proliferation, migration and neuronal differentation of NSPCs. To date, the lack of biomaterials, which facilitate these processes to enhance neural regeneration, is an obstacle for the cell replacement therapies. Our previous study has shown that NSPCs grown on poly-L-ornithine (PO) could proliferate more vigorously and differentiate into more neurons than that on Poly-L-Lysine (PLL) and Fibronectin (FN). Here, we demonstrate that PO could promote migration of NSPCs in vitro, and the underlying mechanism is PO activates α-Actinins 4 (ACTN4), which is firstly certified to be expessed in NSPCs, to promote filopodia formation and therefore enhances NSPCs migration. Taken together, PO might serve as a better candidate for transplanted biomaterials in the regenerative therapeutic strategy, compared with PLL and FN.

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Metrics

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References

Details

Published: Nov 22, 2016
Vol/Issue: 6(1)
License: View

Authors

Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People’s Hospital, Shenzhen, China.

Cite This Article

Hongfei Ge, Anyong Yu, Jingyu Chen, et al. (2016). Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments. Scientific Reports, 6(1). https://doi.org/10.1038/srep37681

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments

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