Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes

Han Yih Lau; Haoqi Wu; Eugene J. H. Wee; Matt Trau; Yuling Wang; Jose R. Botella

doi:10.1038/srep38896

journal article Open Access Jan 17, 2017

Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes

Han Yih Lau Haoqi Wu Eugene J. H. Wee Matt Trau

Yuling Wang

Jose R. Botella

Scientific Reports Vol. 7 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep38896

Abstract

AbstractDeveloping quick and sensitive molecular diagnostics for plant pathogen detection is challenging. Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathogen DNA sequences by recombinase polymerase amplification (RPA) followed by gold nanoparticle-based electrochemical assessment with differential pulse voltammetry (DPV). Our method was 10,000 times more sensitive than conventional polymerase chain reaction (PCR)/gel electrophoresis and could readily identify P. syringae infected plant samples even before the disease symptoms were visible. On the basis of the speed, sensitivity, simplicity and portability of the approach, we believe the method has potential as a rapid disease management solution for applications in agriculture diagnostics.

Topics

No keywords indexed for this article. Browse by subject →

References

30

[1]

Agrios, G. N. Plant pathology. 5th edn, (Elsevier Academic Press, 2005).

[2]

Horsfall, J. G. & Cowling, E. B. Plant disease: an advanced treatise. (Academic Press, 1977).

[3]

Franken, A. A. J. M., Zilverentant, J. F., Boonekamp, P. M. & Schots, A. Specificity of Polyclonal and Monoclonal-Antibodies for the Identification of Xanthomonas-Campestris Pv Campestris. Netherlands Journal of Plant Pathology 98, 81–94, doi: 10.1007/Bf01996321 (1992). 10.1007/bf01996321

[4]

Murphy, K., Travers, P., Walport, M. & Janeway, C. Janeway’s immunobiology. 8th edn, (Garland Science, 2012).

[5]

Ward, E., Foster, S. J., Fraaije, B. A. & McCartney, H. A. Plant pathogen diagnostics: immunological and nucleic acid-based approaches. Ann Appl Biol 145, 1–16, doi: 10.1111/j.1744-7348.2004.tb00354.x (2004). 10.1111/j.1744-7348.2004.tb00354.x

[6]

Vincelli, P. & Tisserat, N. Nucleic acid-based pathogen detection in applied plant pathology. Plant Dis 92, 660–669, doi: 10.1094/Pdis-92-5-0660 (2008). 10.1094/pdis-92-5-0660

[7]

Piepenburg, O., Williams, C. H., Stemple, D. L. & Armes, N. A. DNA detection using recombination proteins. Plos Biol 4, 1115–1121, doi: 10.1371/journal.pbio.0040204 (2006). 10.1371/journal.pbio.0040204

[8]

Ng, B. Y. C., Wee, E. J. H., West, N. P. & Trau, M. Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis. Sci Rep-Uk 5, doi: 10.1038/Srep15027 (2015). 10.1038/srep15027

[9]

Wee, E. J. H., Ngo, T. H. & Trau, M. Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs. Clin Epigenetics 7, doi: 10.1186/s13148-015-0100-6 (2015). 10.1186/s13148-015-0100-6

[10]

Liljander, A. et al. Field-Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp capripneumoniae. J Clin Microbiol 53, 2810–2815, doi: 10.1128/Jcm.00623-15 (2015). 10.1128/jcm.00623-15

[11]

Abd El Wahed, A. et al. Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection. PloS one 10, doi: 10.1371/journal.pone.0129682 (2015). 10.1371/journal.pone.0129682

[12]

Londono, M. A., Harmon, C. L. & Polston, J. E. Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics. Virology journal 13, 48, doi: 10.1186/s12985-016-0504-8 (2016). 10.1186/s12985-016-0504-8

[13]

Drummond, T. G., Hill, M. G. & Barton, J. K. Electrochemical DNA sensors. Nature biotechnology 21, 1192–1199, doi: 10.1038/nbt873 (2003). 10.1038/nbt873

[14]

Lin, C. S. et al. An electrochemical biosensor for the sensitive detection of specific DNA based on a dual-enzyme assisted amplification. Electrochim Acta 147, 785–790, doi: 10.1016/j.electacta.2014.10.092 (2014). 10.1016/j.electacta.2014.10.092

[15]

Yola, M. L., Eren, T. & Atar, N. A novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide. Electrochim Acta 125, 38–47, doi: 10.1016/j.electacta.2014.01.074 (2014). 10.1016/j.electacta.2014.01.074

[16]

Wang, Z. et al. Electrocatalytic oxidation of phytohormone salicylic acid at copper nanoparticles-modified gold electrode and its detection in oilseed rape infected with fungal pathogen Sclerotinia sclerotiorum. Talanta 80, 1277–1281, doi: 10.1016/j.talanta.2009.09.023 (2010). 10.1016/j.talanta.2009.09.023

[17]

Vetrone, S. A., Huarng, M. C. & Alocilja, E. C. Detection of Non-PCR Amplified S. enteritidis Genomic DNA from Food Matrices Using a Gold-Nanoparticle DNA Biosensor: A Proof-of-Concept Study. Sensors-Basel 12, 10487–10499, doi: 10.3390/s120810487 (2012). 10.3390/s120810487

[18]

Kleijn, S. E. F., Lai, S. C. S., Koper, M. T. M. & Unwin, P. R. Electrochemistry of Nanoparticles. Angew Chem Int Edit 53, 3558–3586, doi: 10.1002/anie.201306828 (2014). 10.1002/anie.201306828

[19]

Riley, D. J. Electrochemistry in nanoparticle science. Curr Opin Colloid In 7, 186–192, doi: Pii S1359-0294(02)00047-X, Doi 10.1016/S1359-0294(02)00047-X (2002). 10.1016/s1359-0294(02)00047-x

[20]

Luo, X. L., Morrin, A., Killard, A. J. & Smyth, M. R. Application of nanoparticles in electrochemical sensors and biosensors. Electroanal 18, 319–326, doi: 10.1002/elan.200503415 (2006). 10.1002/elan.200503415

[21]

Lin, L., Liu, Y., Tang, L. H. & Li, J. H. Electrochemical DNA sensor by the assembly of graphene and DNA-conjugated gold nanoparticles with silver enhancement strategy. Analyst 136, 4732–4737, doi: 10.1039/c1an15610a (2011). 10.1039/c1an15610a

[22]

Liu, G. D. et al. Aptamer-Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells. Anal Chem 81, 10013–10018, doi: 10.1021/ac901889s (2009). 10.1021/ac901889s

[23]

Wang, W. T., Fan, X. J., Xu, S. H., Davis, J. J. & Luo, X. L. Low fouling label-free DNA sensor based on polyethylene glycols decorated with gold nanoparticles for the detection of breast cancer biomarkers. Biosens Bioelectron 71, 51–56, doi: 10.1016/j.bios.2015.04.018 (2015). 10.1016/j.bios.2015.04.018

[24]

Iacobellis, N. S. Pseudomonas syringae and related pathogens: biology and genetic. (Kluwer Academic Publishers, 2003).

[25]

Katagiri, F., Thilmony, R. & He, S. Y. The Arabidopsis thaliana-pseudomonas syringae interaction. The Arabidopsis book/American Society of Plant Biologists 1, e0039, doi: 10.1199/tab.0039 (2002). 10.1199/tab.0039

[26]

Trusov, Y. et al. Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling. Plant Physiol 140, 210–220 (2006). 10.1104/pp.105.069625

[27]

Wee, E. J. H., Lau, H. Y., Botella, J. R. & Trau, M. Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings. Chem Commun 51, 5828–5831, doi: 10.1039/c4cc10068a (2015). 10.1039/c4cc10068a

[28]

Frens, G. Controlled Nucleation for Regulation of Particle-Size in Monodisperse Gold Suspensions. Nature-Phys Sci 241, 20–22 (1973). 10.1038/physci241020a0

[29]

Hawkins, T. L., Oconnormorin, T., Roy, A. & Santillan, C. DNA Purification and Isolation Using a Solid-Phase. Nucleic acids research 22, 4543–4544, doi: 10.1093/nar/22.21.4543 (1994). 10.1093/nar/22.21.4543

[30]

Ng, B. Y. C. et al. Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Colloidal Gold Nanoparticles. Anal Chem 87, 10613–10618, doi: 10.1021/acs.analchem.5b03121 (2015). 10.1021/acs.analchem.5b03121

Cited By

169

Current and emerging trends in techniques for plant pathogen detection

Marc Venbrux, Sam Crauwels · 2023

Frontiers in Plant Science

Novel plant disease detection techniques-a brief review

Srividya Attaluri, Rathnaprabha Dharavath · 2023

Molecular Biology Reports

A review of recent advances in plant-pathogen detection systems

Rhea Patel, Bappa Mitra · 2022

Heliyon

Application of lectin-based biosensor technology in the detection of foodborne pathogenic bacteria: a review

Fang Mi, Ming Guan · 2021

The Analyst

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

Kazbek Dyussembayev, Prabhakaran Sambasivam · 2021

Frontiers in Chemistry

Review: a comprehensive summary of a decade development of the recombinase polymerase amplification

Jia Li, Joanne Macdonald · 2019

The Analyst

Metrics

169

Citations

30

References

Details

Published: Jan 17, 2017
Vol/Issue: 7(1)
License: View

Authors

Cite This Article

Han Yih Lau, Haoqi Wu, Eugene J. H. Wee, et al. (2017). Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes. Scientific Reports, 7(1). https://doi.org/10.1038/srep38896

Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes

You May Also Like