Metabolite Profiling of Preneoplastic and Neoplastic Lesions of Oral Cavity Tissue Samples Revealed a Biomarker Pattern

Syed Ghulam Musharraf; Najia Shahid; Syed Muhammad Ali Naqvi; Mahwish Saleem; Amna Jabbar Siddiqui; Anwar Ali

doi:10.1038/srep38985

journal article Open Access Dec 13, 2016

Metabolite Profiling of Preneoplastic and Neoplastic Lesions of Oral Cavity Tissue Samples Revealed a Biomarker Pattern

Syed Ghulam Musharraf

Najia Shahid Syed Muhammad Ali Naqvi Mahwish Saleem Amna Jabbar Siddiqui

Anwar Ali

Scientific Reports Vol. 6 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep38985

Abstract

AbstractOral cancer is a major health challenge in the Indian subcontinent and a dreadful form of cancers worldwide. The current study is focused on the identification of distinguished metabolites of oral cancer tissue samples in comparison with precancerous and control tissue samples using gas chromatography coupled with triple quadrupole tandem mass spectrometry and chemometric analyses. Metabolites obtained were identified through National Institute of Standards and Technology (NIST) mass spectral (Wiley registry) library. Mass Profiler Professional (MPP) software was used for the alignment and for all the statistical analysis. 31 compounds out of 735 found distinguishing among oral cancer, precancerous and control group samples using p-value ≤ 0.05. Partial Least Square Discriminant Analysis (PLSDA) model was generated using statistically significant metabolites gave an overall accuracy of 90.2%. Down-regulated amino acid levels appear to be the result of enhanced energy metabolism or up-regulation of the appropriate biosynthetic pathways, and required cell proliferation in cancer tissues. These results suggest that tissue metabolic profiles have great potential in detecting oral cancer and may aid in understanding its underlying mechanisms.

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Published: Dec 13, 2016
Vol/Issue: 6(1)
License: View

Authors

S

Syed Ghulam Musharraf

N

Najia Shahid

S

Syed Muhammad Ali Naqvi

Cite This Article

Syed Ghulam Musharraf, Najia Shahid, Syed Muhammad Ali Naqvi, et al. (2016). Metabolite Profiling of Preneoplastic and Neoplastic Lesions of Oral Cavity Tissue Samples Revealed a Biomarker Pattern. Scientific Reports, 6(1). https://doi.org/10.1038/srep38985

Metabolite Profiling of Preneoplastic and Neoplastic Lesions of Oral Cavity Tissue Samples Revealed a Biomarker Pattern

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