Long-term exposure to a ‘safe’ dose of bisphenol A reduced protein acetylation in adult rat testes

Zhuo Chen; Xuezhi Zuo; Dongliang He; Shibin Ding; Fangyi Xu; Huiqin Yang; Xin Jin; Ying Fan; Li Ying; Chong Tian; Chenjiang Ying

doi:10.1038/srep40337

journal article Open Access Jan 09, 2017

Long-term exposure to a ‘safe’ dose of bisphenol A reduced protein acetylation in adult rat testes

Zhuo Chen

Xuezhi Zuo Dongliang He Shibin Ding Fangyi Xu Huiqin Yang

Xin Jin

Ying Fan

Li Ying Chong Tian Chenjiang Ying

Scientific Reports Vol. 7 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep40337

Abstract

AbstractBisphenol A (BPA), a typical environmental endocrine-disrupting chemical, induces epigenetic inheritance. Whether histone acetylation plays a role in these effects of BPA is largely unknown. Here, we investigated histone acetylation in male rats after long-term exposure to a ‘safe’ dose of BPA. Twenty adult male rats received either BPA (50 μg/kg·bw/day) or a vehicle diet for 35 weeks. Decreased protein lysine-acetylation levels at approximately ~17 kDa and ~25 kDa, as well as decreased histone acetylation of H3K9, H3K27 and H4K12, were detected by Western blot analysis of testes from the treated rats compared with controls. Additionally, increased protein expression of deacetylase Sirt1 and reduced binding of Sirt1, together with increased binding of estrogen receptor β (ERβ) to caveolin-1 (Cav-1), a structural protein component of caveolar membranes, were detected in treated rats compared with controls. Moreover, decreased acetylation of Cav-1 was observed in the treated rats for the first time. Our study showed that long-term exposure to a ‘safe’ dose of BPA reduces histone acetylation in the male reproductive system, which may be related to the phenotypic paternal-to-offspring transmission observed in our previous study. The evidence also suggested that these epigenetic effects may be meditated by Sirt1 via competition with ERβ for binding to Cav-1.

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Published: Jan 09, 2017
Vol/Issue: 7(1)
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Institut National de la Recherche Scientifique

Cite This Article

Zhuo Chen, Xuezhi Zuo, Dongliang He, et al. (2017). Long-term exposure to a ‘safe’ dose of bisphenol A reduced protein acetylation in adult rat testes. Scientific Reports, 7(1). https://doi.org/10.1038/srep40337

Long-term exposure to a ‘safe’ dose of bisphenol A reduced protein acetylation in adult rat testes

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