Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

Ehsan Shokri; Morteza Hosseini; Mehdi D. Davari; Mohammad R. Ganjali; Maikel P. Peppelenbosch; Farhad Rezaee

doi:10.1038/srep45837

journal article Open Access Apr 07, 2017

Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

Ehsan Shokri Morteza Hosseini

Mehdi D. Davari

Mohammad R. Ganjali Maikel P. Peppelenbosch

Farhad Rezaee

Scientific Reports Vol. 7 No. 1 · Springer Science and Business Media LLC

View at Publisher Save 10.1038/srep45837

Abstract

AbstractA modified non-cross-linking gold-nanoparticles (Au-NPs) aggregation strategy has been developed for the label free colorimetric detection of DNAs/RNAs based on self-assembling target species in the presence of thiolated probes. Two complementary thiol- modified probes, each of which specifically binds at one half of the target introduced SH groups at both ends of dsDNA. Continuous disulfide bond formation at 3′ and 5′ terminals of targets leads to the self-assembly of dsDNAs into the sulfur- rich and flexible products with different lengths. These products have a high affinity for the surface of Au-NPs and efficiently protect the surface from salt induced aggregation. To evaluate the assay efficacy, a small part of the citrus tristeza virus (CTV) genome was targeted, leading to a detection limit of about 5 × 10−9 mol.L−1 over a linear ranged from 20 × 10−9 to 10 × 10−7 mol.L−1. This approach also exhibits good reproducibility and recovery levels in the presence of plant total RNA or human plasma total circulating RNA extracts. Self-assembled targets can be then sensitively distinguished from non-assembled or mismatched targets after gel electrophoresis. The disulfide reaction method and integrating self-assembled DNAs/RNAs targets with bare AuNPs as a sensitive indicator provide us a powerful and simple visual detection tool for a wide range of applications.

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References

Details

Published: Apr 07, 2017
Vol/Issue: 7(1)
License: View

Authors

Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, Aachen 52074, Germany

M

Mohammad R. Ganjali

M

Maikel P. Peppelenbosch

Department of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands

F

Farhad Rezaee

Cite This Article

Ehsan Shokri, Morteza Hosseini, Mehdi D. Davari, et al. (2017). Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles. Scientific Reports, 7(1). https://doi.org/10.1038/srep45837

Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

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