Mastitomics, the integrated omics of bovine milk in an experimental model of
                    Streptococcus uberis
                    mastitis: 3. Untargeted metabolomics

Funmilola Clara Thomas; Manikhandan Mudaliar; Riccardo Tassi; Tom N McNeilly; Richard Burchmore; Karl Burgess; Pawel Herzyk; Ruth N Zadoks; P. David Eckersall

doi:10.1039/c6mb00289g

journal article Open Access Jul 14, 2016

Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics

Funmilola Clara Thomas Manikhandan Mudaliar Riccardo Tassi Tom N McNeilly Richard Burchmore Karl Burgess

Pawel Herzyk Ruth N Zadoks P. David Eckersall

Molecular BioSystems Vol. 12 No. 9 pp. 2762-2769 · Oxford University Press (OUP)

View at Publisher Save 10.1039/c6mb00289g

Abstract

Abstract
Intramammary infection leading to bovine mastitis is the leading disease problem affecting dairy cows and has marked effects on the milk produced by infected udder quarters. An experimental model of Streptococcus uberis mastitis has previously been investigated for clinical, immunological and pathophysiological alteration in milk, and has been the subject of peptidomic and quantitative proteomic investigation. The same sample set has now been investigated with a metabolomics approach using liquid chromatography and mass spectrometry. The analysis revealed over 3000 chromatographic peaks, of which 690 were putatively annotated with a metabolite. Hierarchical clustering analysis and principal component analysis demonstrated that metabolite changes due to S. uberis infection were maximal at 81 hours post challenge with metabolites in the milk from the resolution phase at 312 hours post challenge being closest to the pre-challenge samples. Metabolic pathway analysis revealed that the majority of the metabolites mapped to carbohydrate and nucleotide metabolism show a decreasing trend in concentration up to 81 hours post-challenge whereas an increasing trend was found in lipid metabolites and di-, tri- and tetra-peptides up to the same time point. The increase in these peptides coincides with an increase in larger peptides found in the previous peptidomic analysis and is likely to be due to protease degradation of milk proteins. Components of bile acid metabolism, linked to the FXR pathway regulating inflammation, were also increased. Metabolomic analysis of the response in milk during mastitis provides an essential component to the full understanding of the mammary gland's response to infection.

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References

50

[1]

Tassi J. Dairy Sci. (2013) 10.3168/jds.2013-6741

[2]

Thomas Mol. BioSyst. (2016)

[3]

Mudaliar Mol. BioSyst. (2016)

[4]

Fischer Proteomics (2013) 10.1002/pmic.201300192

[5]

Fillet Drug Discovery Today: Technol. (2015) 10.1016/j.ddtec.2015.01.006

[6]

Roessner Biotechniques (2009) 10.2144/000113133

[7]

Wishart Nat. Rev. Drug Discovery (2016)

[8]

Boudonck Metabolomics (2009) 10.1007/s11306-009-0160-8

[9]

Klein J. Dairy Sci. (2010) 10.3168/jds.2009-2563

[10]

Lamanna Magn. Reson. Chem. (2011)

[11]

U. K. Sundekilde , Milk metabolite variability and heritability and their association with technological properties of bovine milk elucidated by NMR-based metabonomics, PhD thesis, @Århus University, Department of Food Science, @Årslev, 2012.

[12]

Sundekilde Metabolites (2013) 10.3390/metabo3020204

[13]

Hettinga J. Dairy Sci. (2009) 10.3168/jds.2008-1818

[14]

Hettinga J. Dairy Sci. (2008) 10.3168/jds.2007-0941

[15]

Hettinga Vet. Microbiol. (2009) 10.1016/j.vetmic.2009.01.016

[16]

Hettinga J. Dairy Sci. (2015) 10.3168/jds.2015-9720

[17]

Nuclear magnetic resonance metabonomics reveals strong association between milk metabolites and somatic cell count in bovine milk

U.K. Sundekilde, N.A. Poulsen, L.B. Larsen et al.

Journal of Dairy Science 2013 10.3168/jds.2012-5819

[18]

Zadoks CAB Rev. (2007)

[19]

Beltran Anal. Chem. (2012) 10.1021/ac3005567

[20]

Canelas Anal. Chem. (2009) 10.1021/ac900999t

[21]

Pluskal BMC Bioinf. (2010) 10.1186/1471-2105-11-395

[22]

Creek Bioinformatics (2012) 10.1093/bioinformatics/bts069

[23]

Holman Curr. Protoc. Bioinformatics (2014) 10.1002/0471250953.bi1324s46

[24]

Tautenhahn BMC Bioinf. (2008) 10.1186/1471-2105-9-504

[25]

Scheltema Anal. Chem. (2011) 10.1021/ac2000994

[26]

Salek GigaScience (2013) 10.1186/2047-217x-2-13

[27]

Proposed minimum reporting standards for chemical analysis

Lloyd W. Sumner, Alexander Amberg, Dave Barrett et al.

Metabolomics 2007 10.1007/s11306-007-0082-2

[28]

Creek Anal. Chem. (2011) 10.1021/ac2021823

[29]

Speakman Stem Cells (2014) 10.1002/stem.1761

[30]

Leader Rapid Commun. Mass Spectrom. (2011) 10.1002/rcm.5245

[31]

Yamada Nucleic Acids Res. (2011) 10.1093/nar/gkr313

[32]

KEGG as a reference resource for gene and protein annotation

Minoru Kanehisa, Yoko Sato, Masayuki Kawashima et al.

Nucleic Acids Research 2016 10.1093/nar/gkv1070

[33]

Nuclear magnetic resonance metabonomics reveals strong association between milk metabolites and somatic cell count in bovine milk

U.K. Sundekilde, N.A. Poulsen, L.B. Larsen et al.

Journal of Dairy Science 2013 10.3168/jds.2012-5819

[34]

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES

Jordi Folch, M. Lees, G.H. Sloane Stanley

Journal of Biological Chemistry 1957 10.1016/s0021-9258(18)64849-5

[35]

Reis J. Lipid Res. (2013) 10.1194/jlr.m034330

[36]

Hofmann Proc. Natl. Acad. Sci. U. S. A. (2006) 10.1073/pnas.0600780103

[37]

Sung Dig. Dis. Sci. (1993) 10.1007/bf01297092

[38]

Calmus Clin. Res. Hepatol. Gastroenterol. (2014) 10.1016/j.clinre.2014.07.007

[39]

Chiang Compr. Physiol. (2013) 10.1002/j.2040-4603.2013.tb00517.x

[40]

Sipka Int. Arch. Allergy Immunol. (2014) 10.1159/000366100

[41]

Wang Hepatology (2008) 10.1002/hep.22519

[42]

Zhang Pharmacogenomics (2008) 10.2217/14622416.9.11.1695

[43]

Zhang J. Immunol. (2012) 10.4049/jimmunol.1102412

[44]

Hogenauer J. Med. Chem. (2014) 10.1021/jm501052c

[45]

Duboc Dig. Liver Dis. (2014) 10.1016/j.dld.2013.10.021

[46]

Ogola J. Vet. Sci. (2007) 10.4142/jvs.2007.8.3.237

[47]

Malek dos Reis BMC Vet. Res. (2013) 10.1186/1746-6148-9-67

[48]

Larsen Animal (2010) 10.1017/s1751731110000947

[49]

Haddadi Reprod., Nutr., Dev. (2005) 10.1051/rnd:2005033

[50]

Prostaglandins and glutathione peroxidase in bovine mastitis

F. ATROSHI, J. PARANTAINEN, S. SANKARI et al.

Research in Veterinary Science 1986 10.1016/s0034-5288(18)30551-4

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References

Details

Published: Jul 14, 2016
Vol/Issue: 12(9)
Pages: 2762-2769
License: View

Authors

F

Funmilola Clara Thomas

Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow a , Bearsden Road, Glasgow, G61 1QH, UK david.eckersall@glasgow.ac.uk

M

Manikhandan Mudaliar

Glasgow Polyomics, College of Medical, Veterinary and Life Science, University of Glasgow b , Glasgow, UK; Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow a , Bearsden Road, Glasgow, G61 1QH, UK david.eckersall@glasgow.ac.uk

R

Riccardo Tassi

Moredun Research Institute c , Pentlands Science Park Bush Loan, Penicuik UK

T

Tom N McNeilly

Moredun Research Institute c , Pentlands Science Park Bush Loan, Penicuik UK

R

Richard Burchmore

Institute of Infection, Immunity and Inflammation, University of Glasgow d , Glasgow, UK; Glasgow Polyomics, College of Medical, Veterinary and Life Science, University of Glasgow b , Glasgow, UK

K

Karl Burgess

Institute of Infection, Immunity and Inflammation, University of Glasgow d , Glasgow, UK; Glasgow Polyomics, College of Medical, Veterinary and Life Science, University of Glasgow b , Glasgow, UK

P

Pawel Herzyk

Institute of Molecular Cell and Systems Biology, University of Glasgow e , Glasgow, UK; Glasgow Polyomics, College of Medical, Veterinary and Life Science, University of Glasgow b , Glasgow, UK

R

Ruth N Zadoks

Moredun Research Institute c , Pentlands Science Park Bush Loan, Penicuik UK; Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow a , Bearsden Road, Glasgow, G61 1QH, UK david.eckersall@glasgow.ac.uk

P

P. David Eckersall

Institute of Biodiversity Animal Health and Comparative Medicine, University of Glasgow a , Bearsden Road, Glasgow, G61 1QH, UK david.eckersall@glasgow.ac.uk

Funding

Wellcome Trust Award: 097821/Z/11/Z

Cite This Article

Funmilola Clara Thomas, Manikhandan Mudaliar, Riccardo Tassi, et al. (2016). Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics. Molecular BioSystems, 12(9), 2762-2769. https://doi.org/10.1039/c6mb00289g

Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics

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