A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity

We show that the polarising agent SNAPol-1 enters human cells yielding 800 MHz DNP solid-state NMR data of remarkable sensitivity & resolution thereby revealing structural heterogeneity of ubiquitin at atomic scale and with sub-cellular specificity.

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Details

Published: Jan 01, 2023
Vol/Issue: 14(36)
Pages: 9892-9899
License: View

Authors

D

David Beriashvili

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

R

Ru Yao

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China

F

Francesca D'Amico

Department of Cell and Chemical Biology, Leiden University Medical Center (LUMC), Einthovenweg 20, 2333 ZC, Leiden, The Netherlands

M

Michaela Krafčíková

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

A

Andrei Gurinov

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

A

Adil Safeer

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

X

Xinyi Cai

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China

M

Monique P. C. Mulder

Department of Cell and Chemical Biology, Leiden University Medical Center (LUMC), Einthovenweg 20, 2333 ZC, Leiden, The Netherlands

G

Gert E. Folkers

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

M

Marc Baldus

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

Funding

National Natural Science Foundation of China Award: 21871210, 22174099

European Commission Award: 871037

Nederlandse Organisatie voor Wetenschappelijk Onderzoek Award: 175.010.2009.002

H2020 Marie Skłodowska-Curie Actions Award: 813599

Cite This Article

David Beriashvili, Ru Yao, Francesca D'Amico, et al. (2023). A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity. Chemical Science, 14(36), 9892-9899. https://doi.org/10.1039/d3sc02117c

A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity

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