Liquid–liquid phase separation-assisted Raman microscopy for sensitive and label-free analysis of enzymatic reactions and protein–small molecule interactions

Lisa Kageyama; Shinya Tahara; Reona Tobita; Shinji Kajimoto; Takakazu Nakabayashi

doi:10.1039/d5an00973a

journal article Jan 01, 2026

Liquid–liquid phase separation-assisted Raman microscopy for sensitive and label-free analysis of enzymatic reactions and protein–small molecule interactions

Lisa Kageyama

Shinya Tahara

Reona Tobita Shinji Kajimoto

Takakazu Nakabayashi

The Analyst Vol. 151 No. 3 pp. 807-815 · Royal Society of Chemistry (RSC)

View at Publisher Save 10.1039/d5an00973a

Abstract

High-concentration PEG-based liquid–liquid phase separation concentrates biomolecules into droplets, enabling highly sensitive, label-free Raman analysis of enzymatic reactions and protein–small-molecule interactions in dilute, small-volume samples.

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References

30

[1]

Potential of Raman spectroscopic techniques to study proteins

Nikki Kuhar, Sanchita Sil, Siva Umapathy

Spectrochimica Acta Part A: Molecular and Biomolec... 2021 10.1016/j.saa.2021.119712

[2]

Pezzotti J. Raman Spectrosc. (2021) 10.1002/jrs.6204

[3]

Unveiling the Molecular Secrets: A Comprehensive Review of Raman Spectroscopy in Biological Research

Anshuman Chandra, Vimal Kumar, Umesh Chandra Garnaik et al.

ACS Omega 2024 10.1021/acsomega.4c00591

[4]

Cialla-May Anal. Chem. (2022) 10.1021/acs.analchem.1c03235

[5]

Kamp Nat. Commun. (2024) 10.1038/s41467-024-49403-w

[6]

Wang Analyst (2025) 10.1039/d4an01409j

[7]

Pastrana-Otero Analyst (2025) 10.1039/d4an00800f

[8]

Watson Curr. Opin. Struct. Biol. (2025) 10.1016/j.sbi.2025.103041

[9]

Tobita Chem. Commun. (2024) 10.1039/d4cc03035d

[10]

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

Simon Alberti, Amy Gladfelter, Tanja Mittag

Cell 2019 10.1016/j.cell.2018.12.035

[11]

Zhou Science (2022) 10.1126/science.abn5582

[12]

Dada Nat. Commun. (2024) 10.1038/s41467-024-47585-x

[13]

Ananthapadmanabhan Langmuir (1987) 10.1021/la00073a005

[14]

Phosphoric acid partition in aqueous two phase systems

Guadalupe Falcon-Millan, M. Pilar Gonzalez-Muñoz, Alain Durand et al.

Journal of Molecular Liquids 2017 10.1016/j.molliq.2017.06.085

[15]

Comparison between Protein−Polyethylene Glycol (PEG) Interactions and the Effect of PEG on Protein−Protein Interactions Using the Liquid−Liquid Phase Transition

Ying Wang, Onofrio Annunziata

The Journal of Physical Chemistry B 2007 10.1021/jp065608u

[16]

Testa Nat. Commun. (2021) 10.1038/s41467-021-26532-0

[17]

Poudyal Nat. Commun. (2023) 10.1038/s41467-023-41864-9

[18]

Murakami Chem. Sci. (2021) 10.1039/d0sc06095j

[19]

Yokosawa J. Phys. Chem. Lett. (2022) 10.1021/acs.jpclett.2c00962

[20]

Yokosawa Chem. Phys. Lett. (2023) 10.1016/j.cplett.2023.140634

[21]

Kageyama Chem. Phys. Lett. (2024) 10.1016/j.cplett.2024.141671

[22]

Morla-Folch ACS Nano (2016) 10.1021/acsnano.5b07966

[23]

Guesdon J. Histochem. Cytochem. (1979) 10.1177/27.8.90074

[24]

Fagnano J. Raman Spectrosc. (1995) 10.1002/jrs.1250261103

[25]

Altangerel Proc. Natl. Acad. Sci. U. S. A. (2023) 10.1073/pnas.2218826120

[26]

Rosario-Alomar J. Phys. Chem. B (2015) 10.1021/jp508471v

[27]

Dóka Sci. Adv. (2020) 10.1126/sciadv.aax8358

[28]

Matsunaga Nat. Commun. (2023) 10.1038/s41467-023-40182-4

[29]

Iqbal Biol. Proced. Online (2016) 10.1186/s12575-016-0048-8

[30]

Zhang RSC Adv. (2025) 10.1039/d4ra08232j

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Details

Published: Jan 01, 2026
Vol/Issue: 151(3)
Pages: 807-815
License: View

Authors

L

Lisa Kageyama