Digital fluorescent pH probes: polymer-based design, fluorescence response, mechanism, functional tuning and application to logic operation in live cells

Seiichi Uchiyama; Eiko Hamada; Masaharu Takarada; Kohki Okabe

doi:10.1039/d5an01187f

journal article Open Access Jan 01, 2026

Digital fluorescent pH probes: polymer-based design, fluorescence response, mechanism, functional tuning and application to logic operation in live cells

Seiichi Uchiyama

Eiko Hamada

Masaharu Takarada

Kohki Okabe

The Analyst Vol. 151 No. 7 pp. 1978-1991 · Royal Society of Chemistry (RSC)

View at Publisher Save 10.1039/d5an01187f

Abstract

We developed polymers that exhibit digital-type fluorescence responses to pH variations in aqueous solution and applied them to intracellular logic operations.

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Details

Published: Jan 01, 2026
Vol/Issue: 151(7)
Pages: 1978-1991
License: View

Authors

S

Seiichi Uchiyama

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

E

Eiko Hamada

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

M

Masaharu Takarada

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

K

Kohki Okabe

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Funding

Japan Society for the Promotion of Science Award: 24655059

Iketani Science and Technology Foundation Award: 0251023-A

Ogasawara Foundation for the Promotion of Science and Engineering

Foundation for Promotion of Material Science and Technology of Japan

Cite This Article

Seiichi Uchiyama, Eiko Hamada, Masaharu Takarada, et al. (2026). Digital fluorescent pH probes: polymer-based design, fluorescence response, mechanism, functional tuning and application to logic operation in live cells. The Analyst, 151(7), 1978-1991. https://doi.org/10.1039/d5an01187f

Digital fluorescent pH probes: polymer-based design, fluorescence response, mechanism, functional tuning and application to logic operation in live cells

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