Sequential intracellular delivery of genetic coding molecules using an acoustic electric microfluidic platform

Michelle Zhang; Aida Z. Taravatfard; Mohammad Aghaamoo; Abraham P. Lee

doi:10.1039/d5lc00941c

journal article Jan 01, 2026

Sequential intracellular delivery of genetic coding molecules using an acoustic electric microfluidic platform

Michelle Zhang

Aida Z. Taravatfard Mohammad Aghaamoo

Abraham P. Lee

Lab on a Chip · Royal Society of Chemistry (RSC)

View at Publisher Save 10.1039/d5lc00941c

Abstract

Acoustic microstreaming vortices enable high-throughput cell trapping and sequential delivery of plasmid DNA and Cas9 RNP, boosting transfection up to sevenfold by eliminating cargo competition and/or interaction.

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Details

Published: Jan 01, 2026
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Authors

M

Michelle Zhang

School of Pharmacy and Pharmaceutical Sciences, University of California, Irvine, CA 92697, USA

A

Aida Z. Taravatfard

Department of Biosystems, University of Leuven, Willem de Croylaan 42, 3001, Leuven, Belgium

M

Mohammad Aghaamoo

Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA

A

Abraham P. Lee

Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA; Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA, 92697, USA

Funding

National Institute of General Medical Sciences Award: R01GM145987

Cite This Article

Michelle Zhang, Aida Z. Taravatfard, Mohammad Aghaamoo, et al. (2026). Sequential intracellular delivery of genetic coding molecules using an acoustic electric microfluidic platform. Lab on a Chip. https://doi.org/10.1039/d5lc00941c

Sequential intracellular delivery of genetic coding molecules using an acoustic electric microfluidic platform

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