Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes

Luba Metlitskaia; Jennifer E. Cabralda; Dinar Suleman; Cynthia Kerry; Jacquelyn Brinkman; Daniel Bartfeld; M. Marta Guarna

doi:10.1042/ba20030100

journal article Jun 01, 2004

Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes

Luba Metlitskaia Jennifer E. Cabralda Dinar Suleman Cynthia Kerry Jacquelyn Brinkman Daniel Bartfeld M. Marta Guarna

Biotechnology and Applied Biochemistry Vol. 39 No. 3 pp. 339-345 · Wiley

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Abstract

Endogenous antimicrobial peptides are ubiquitous components of animal and plant host defences. These peptides, usually cationic and amphipathic, kill target cells rapidly and are efficacious against antibiotic‐resistant and clinically relevant pathogens. A practical challenge in the development of cationic peptides as therapeutics is to meet the production requirements for large quantities of highly purified drug substance at competitive costs. While chemical peptide synthesis can be used to manufacture cationic peptides, we have developed cost‐effective methods for recombinant production by expressing fusion proteins comprised of multiple copies of the peptides. The fusion proteins accumulate in Escherichia coli inclusion bodies and constitute over 50% of the total cellular proteins. Active antimicrobial peptides are released by chemical reagents and purified by chromatography, combining both standard and novel approaches. Challenges of industrial‐scale manufacturing of therapeutics were considered in the development of this process.

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Details

Published: Jun 01, 2004
Vol/Issue: 39(3)
Pages: 339-345
License: View

Authors

Cite This Article

Luba Metlitskaia, Jennifer E. Cabralda, Dinar Suleman, et al. (2004). Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes. Biotechnology and Applied Biochemistry, 39(3), 339-345. https://doi.org/10.1042/ba20030100

Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes

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