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journal article Sep 01, 1998

Cytokine-stimulated release of decay-accelerating factor (DAF; CD55) from HT-29 human intestinal epithelial cells

J Nasu M Mizuno T Uesu K Takeuchi T Inaba S Ohya M Kawada K Shimo H Okada T Fujita T Tsuji
Clinical and Experimental Immunology Vol. 113 No. 3 pp. 379-385 · Oxford University Press (OUP)
View at Publisher Save 10.1046/j.1365-2249.1998.00660.x
Abstract
SUMMARYExpression of DAF (CD55) is enhanced on colonic epithelial cells of patients with ulcerative colitis (UC), and stool DAF concentrations are increased in patients with active disease. Cytokines are known to modulate DAF expression in various human cells, and lesions of UC reveal altered profiles of cytokine production. In this study, we evaluate the effects of various cytokines, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, and interferon-gamma (IFN-γ), on the synthesis and kinetics of DAF protein in HT-29 human intestinal epithelial cells. Using flow cytometry and an ELISA, we found that HT-29 cells constitutively express DAF on the cell surface and spontaneously release DAF into the culture supernatant under standard culture conditions. When the culture supernatant was centrifuged at 100 000 g, nearly a half of DAF was precipitated, indicating that one half of the released DAF was present as a membrane-bound form and the other half as a soluble form. Analysis of the culture supernatant of biotin surface-labelled HT-29 cells suggested that the soluble form DAF was derived by secretion from within the cell or by cleavage from the cell surface. Among the cytokines, IL-4 markedly, and IL-1β moderately, enhanced the expression and the release of DAF. Actinomycin D, cycloheximide, and brefeldin A inhibited the increase in DAF release induced by IL-4 and IL-1β stimulation. These results suggest that DAF is released from intestinal epithelial cells in response to cytokine stimulation and that IL-4 and IL-1β are possible cytokines involved in DAF release into the colonic lumen of patients with UC.
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Metrics
40
Citations
27
References
Details
Published
Sep 01, 1998
Vol/Issue
113(3)
Pages
379-385
License
View
Authors
J
J Nasu

First Department of Internal Medicine, Okayama University Medical School, Okayama

M
M Mizuno

First Department of Internal Medicine, Okayama University Medical School, Okayama

T
T Uesu

First Department of Internal Medicine, Okayama University Medical School, Okayama

K
K Takeuchi

First Department of Internal Medicine, Okayama University Medical School, Okayama

T
T Inaba

First Department of Internal Medicine, Okayama University Medical School, Okayama

S
S Ohya

First Department of Internal Medicine, Okayama University Medical School, Okayama

M
M Kawada

First Department of Internal Medicine, Okayama University Medical School, Okayama

K
K Shimo

First Department of Internal Medicine, Okayama University Medical School, Okayama

H
H Okada

First Department of Internal Medicine, Okayama University Medical School, Okayama

T
T Fujita

Department of Biochemistry, Fukushima Medical College, Fukushima, Japan

T
T Tsuji

First Department of Internal Medicine, Okayama University Medical School, Okayama

Cite This Article
J Nasu, M Mizuno, T Uesu, et al. (1998). Cytokine-stimulated release of decay-accelerating factor (DAF; CD55) from HT-29 human intestinal epithelial cells. Clinical and Experimental Immunology, 113(3), 379-385. https://doi.org/10.1046/j.1365-2249.1998.00660.x
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