Improved stability of free-standing lipid bilayers based on nanoporous alumina films

Ayumi Hirano-Iwata; Tasuku Taira; Azusa Oshima; Yasuo Kimura; Michio Niwano

doi:10.1063/1.3441298

journal article May 24, 2010

Improved stability of free-standing lipid bilayers based on nanoporous alumina films

Ayumi Hirano-Iwata

Tasuku Taira Azusa Oshima

Yasuo Kimura Michio Niwano

Applied Physics Letters Vol. 96 No. 21 · AIP Publishing

View at Publisher Save 10.1063/1.3441298

Abstract

In this study, we propose a method for improving the stability of free-standing bilayer lipid membranes (BLMs) by preparing BLMs across nanoporous alumina films. The use of porous alumina reduced individual membrane size to improve the BLM stability. The BLMs were stable under an applied voltage of ±1 V. The lifetime of the BLMs was 16–30 h with and without incorporated gramicidin channels. Electric properties of the BLMs as a platform for channel-current recordings are also discussed. Since the total area of the BLMs is still large to facilitate protein incorporation, our approach is useful for designing highly sensitive biosensors based on channel proteins.

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References

Details

Published: May 24, 2010
Vol/Issue: 96(21)

Authors

A

Ayumi Hirano-Iwata

Tohoku University 1 Graduate School of Biomedical Engineering, , 6-6 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Japan Science and Technology Agency (JST) 2 PRESTO, , 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan

T

Tasuku Taira

Tohoku University 3 Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, , 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan

A

Azusa Oshima

Tohoku University 1 Graduate School of Biomedical Engineering, , 6-6 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan

Y

Yasuo Kimura

Tohoku University 3 Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, , 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan

M

Michio Niwano

Tohoku University 1 Graduate School of Biomedical Engineering, , 6-6 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Tohoku University 3 Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, , 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan

Cite This Article

Ayumi Hirano-Iwata, Tasuku Taira, Azusa Oshima, et al. (2010). Improved stability of free-standing lipid bilayers based on nanoporous alumina films. Applied Physics Letters, 96(21). https://doi.org/10.1063/1.3441298

Improved stability of free-standing lipid bilayers based on nanoporous alumina films

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