Cascade amplification-based triple probe biosensor for high-precision DNA hybridization detection of lung cancer gene

Zhiyong Yin; Xili Jing; Shuguang Li

doi:10.1063/5.0228760

journal article Open Access Sep 01, 2024

Cascade amplification-based triple probe biosensor for high-precision DNA hybridization detection of lung cancer gene

Zhiyong Yin

Xili Jing

Shuguang Li

APL Photonics Vol. 9 No. 9 · AIP Publishing

View at Publisher Save 10.1063/5.0228760

Abstract

As an essential biomarker for diagnosing and treating various diseases, low-cost, quantitative detection methods for complementary DNA (cDNA) have received much attention. The surface plasmon resonance (SPR) sensing technique is an effective measurement scheme, but the ambient temperature and pH variations have a non-negligible impact. In this work, we developed a triple-probe SPR sensing system for detecting cDNA concentration, temperature, and pH. In order to satisfy the triple parameter measurements, we used a microstructured optical fiber as the sensing platform, silver and gold films as the excitation layer, and a MoS2 film as the modulation layer. First, we explore the modulation mechanism of SPR and the conditions for excitation of triple SPR and demonstrate that the carrier concentration is a crucial factor affecting the resonance wavelength. Then, the feasibility of the sensing system for triple-probing is theoretically analyzed. Finally, in the experiment, the optimal parameters of the sensor were determined, and the triple parameter detection was successfully realized. The experimental results show that the three probes can work independently, and the hybridized DNA probe can realize the selective detection of cDNA with a sensitivity of 0.249 nm/(nmol/l). The maximum sensitivity of the pH probe and the temperature probe are 51.5 nm/pH and 6.14 nm/°C. In addition, the experimental results show that the sensing probes have excellent reproducibility. This paper’s innovation is using the fiber optic SPR effect to achieve quantitative detection for cDNA, temperature detection, and pH detection. Therefore, the sensor has a promising future in early diagnosis and biosensing.

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References

Details

Published: Sep 01, 2024
Vol/Issue: 9(9)
License: View

Authors

Z

Zhiyong Yin

State Key Laboratory of Metastable Materials Science and Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University , Qinhuangdao 066004, People’s Republic of China

X

Xili Jing

State Key Laboratory of Metastable Materials Science and Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University , Qinhuangdao 066004, People’s Republic of China

S

Shuguang Li

State Key Laboratory of Metastable Materials Science and Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University , Qinhuangdao 066004, People’s Republic of China

Funding

National Natural Science Foundation of China Award: 42174162

Postgraduate Innovation Funding Project of Hebei Province Award: CXZZBS2024062

Cite This Article

Zhiyong Yin, Xili Jing, Shuguang Li (2024). Cascade amplification-based triple probe biosensor for high-precision DNA hybridization detection of lung cancer gene. APL Photonics, 9(9). https://doi.org/10.1063/5.0228760

Cascade amplification-based triple probe biosensor for high-precision DNA hybridization detection of lung cancer gene

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