Microarray analysis of bast fibre producing tissues of Cannabis sativa identifies transcripts associated with conserved and specialised processes of secondary wall development

Mary A. De Pauw; John J. Vidmar; JoAnn Collins; Rick A. Bennett; Michael K. Deyholos

doi:10.1071/fp07014

journal article Jul 23, 2007

Microarray analysis of bast fibre producing tissues of Cannabis sativa identifies transcripts associated with conserved and specialised processes of secondary wall development

Mary A. De Pauw John J. Vidmar JoAnn Collins Rick A. Bennett Michael K. Deyholos

Functional Plant Biology Vol. 34 No. 8 pp. 737-749 · CSIRO Publishing

View at Publisher Save 10.1071/fp07014

Abstract

The mechanisms underlying bast fibre differentiation in hemp (Cannabis sativa L.) are largely unknown. We hybridised a cDNA microarray with RNA from fibre enriched tissues extracted at three different positions along the stem axis. Accordingly, we identified transcripts that were enriched in tissues in which phloem fibres were elongating or undergoing secondary wall thickening. These results were consistent with a dynamic pattern of cell wall deposition involving tissue specific expression of a large set of distinct glycosyltransferases and glycosylhydrolases apparently acting on polymers containing galactans, mannans, xylans, and glucans, as well as raffinose-series disaccharides. Putative arabinogalactan proteins and lipid transfer proteins were among the most highly enriched transcripts in various stem segments, with different complements of each expressed at each stage of development. We also detected stage-specific expression of brassinosteroid-related transcripts, various transporters, polyamine and phenylpropanoid related genes, and seven putative transcription factors. Finally, we observed enrichment of many transcripts with unknown biochemical function, some of which had been previously implicated in fibre development in poplar or cotton. Together these data complement and extend existing biochemical models of bast fibre development and secondary wall deposition and highlight uncharacterised, but conserved, components of these processes.

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Cited By

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Microarray Analysis of Developing Flax Hypocotyls Identifies Novel Transcripts Correlated with Specific Stages of Phloem Fibre Differentiation

Melissa J. Roach, Michael K. Deyholos · 2008

Annals of Botany

Metrics

23

Citations

49

References

Details

Published: Jul 23, 2007
Vol/Issue: 34(8)
Pages: 737-749
License: View

Authors

M

Mary A. De Pauw

ADepartment of Biological Sciences, University of Alberta, Edmonton, T6G 2E9 Canada.

J

John J. Vidmar

BAlberta Research Council, Vegreville, T9C 1T4 Canada.

J

JoAnn Collins

ADepartment of Biological Sciences, University of Alberta, Edmonton, T6G 2E9 Canada.

R

Rick A. Bennett

ADepartment of Biological Sciences, University of Alberta, Edmonton, T6G 2E9 Canada.

M

Michael K. Deyholos

ADepartment of Biological Sciences, University of Alberta, Edmonton, T6G 2E9 Canada.; CCorresponding author. Email: deyholos@ualberta.ca

Cite This Article

Mary A. De Pauw, John J. Vidmar, JoAnn Collins, et al. (2007). Microarray analysis of bast fibre producing tissues of Cannabis sativa identifies transcripts associated with conserved and specialised processes of secondary wall development. Functional Plant Biology, 34(8), 737-749. https://doi.org/10.1071/fp07014

Microarray analysis of bast fibre producing tissues of Cannabis sativa identifies transcripts associated with conserved and specialised processes of secondary wall development

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